ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations

Kim, Taewoo; Serabyn, Eugene; Schadegg, Maximilian; Liewer, K. M.; Oborny, Nathan; Wallace, J. Kent; Rider, Stephanie; Bedrossian, Manuel; Lindensmith, Christian; Nadeau, Jay

doi:10.1017/s1551929520000899

Cited by 6 publications

(5 citation statements)

References 16 publications

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“…In transporting the system, there was no special care taken other than placing the microscope into a foam-filled carrying case to keep it free from vibrations and shock. Once the microscope was set up at the lab, we performed initial tests using microbeads and U.S. Air Force (USAF) targets, as described in our previous article, to confirm that the transport caused no misalignment [1].…”

Section: Methodsmentioning

confidence: 99%

“…The Extant Life Volumetric Imaging System (ELVIS) is a combined digital holographic microscope (DHM) and fluorescence light-field microscope (FLFM) that uses the same objectives and sample chamber to provide overlapping volumes of view for instantaneous 3D measurement in amplitude, quantitative phase, and fluorescence. The construction and operation of the system is described in the May 2020 issue of Microscopy Today [1].…”

Section: Introductionmentioning

confidence: 99%

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ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations – Field Demonstration

et al. 2020

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations – Field Demonstration

et al. 2020

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“…OWLS is a mature, Technology Readiness Level (TRL) 5, suite of six integrated instruments and onboard software designed to capture and recognize a range of chemical and biological biosignatures in liquid water samples (Lindensmith et al 2022). Three microscopes focus on cellular-scale, biological biosignatures within the same sample volume of water: a Digital Holographic Microscope (DHM) to capture video of selfpropelled motility at 185 nm scales, a co-observing Fluorescence Light-Field Microscope (FLFM) to capture video of compounds related to cellular walls, proteins, and nucleic acids at 230 nm scales, and a separate High Resolution Fluorescent Imager (HRFI) that captures complimentary still imagery similar to the FLFM at 60 nm scales (Bedrossian et al 2017;Serabyn et al 2019;Kim et al 2020). OWLS also includes three capillary electrophoresis instruments focused on molecular-scale, chemical biosignatures: an ElectroSpray Ionization Mass Spectrometer (ESI-MS) detects biomolecules such as amino acids, the Laser Induced Fluorescence (LIF) instrument analyzes the distribution of chirality in amino acids, and the Capacitively Coupled Contactless Conductivity Detection (C4D) detects organic molecules important to terrestrial metabolism such as tricarboxylic acid and phosphate (Jaramillo et al 2021;Oborny et al 2021;Mora et al 2022;Willis et al 2022).…”

Section: The Ocean Worlds Life Surveyormentioning

confidence: 99%

Onboard Science Instrument Autonomy for the Detection of Microscopy Biosignatures on the Ocean Worlds Life Surveyor

Wronkiewicz,

Lee,

Mandrake

et al. 2024

Planet. Sci. J.

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The quest to find extraterrestrial life is a critical scientific endeavor with civilization-level implications. Icy moons in our solar system are promising targets for exploration because their liquid oceans make them potential habitats for microscopic life. However, the lack of a precise definition of life poses a fundamental challenge to formulating detection strategies. To increase the chances of unambiguous detection, a suite of complementary instruments must sample multiple independent biosignatures (e.g., composition, motility/behavior, and visible structure). Such an instrument suite could generate 10,000× more raw data than is possible to transmit from distant ocean worlds like Enceladus or Europa. To address this bandwidth limitation, Onboard Science Instrument Autonomy (OSIA) is an emerging discipline of flight systems capable of evaluating, summarizing, and prioritizing observational instrument data to maximize science return. We describe two OSIA implementations developed as part of the Ocean World Life Surveyor (OWLS) prototype instrument suite at the Jet Propulsion Laboratory. The first identifies life-like motion in digital holographic microscopy videos, and the second identifies cellular structure and composition via innate and dye-induced fluorescence. Flight-like requirements and computational constraints were used to lower barriers to infusion, similar to those available on the Mars helicopter, “Ingenuity.” We evaluated the OSIA's performance using simulated and laboratory data and conducted a live field test at the hypersaline Mono Lake planetary analog site. Our study demonstrates the potential of OSIA for enabling biosignature detection and provides insights and lessons learned for future mission concepts aimed at exploring the outer solar system.

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“…Several groups are funded to develop microscopes specifically for astrobiological (rather than geochemical) investigations, microscopes that utilize multiple excitation wavelengths (including deep-UV) to excite biological autofluorescence and fluorescent stains that target specific structural and functional biomarkers (membrane lipids and proteins) (Quinn et al , 2019 ) and digital holographic imaging (Kim et al , 2020 ).…”

Section: Traceability To the Science Payloadmentioning

confidence: 99%

Science Objectives for Flagship-Class Mission Concepts for the Search for Evidence of Life at Enceladus

et al. 2022

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Cassini revealed that Saturn's Moon Enceladus hosts a subsurface ocean that meets the accepted criteria for habitability with bio-essential elements and compounds, liquid water, and energy sources available in the environment. Whether these conditions are sufficiently abundant and collocated to support life remains unknown and cannot be determined from Cassini data. However, thanks to the plume of oceanic material emanating from Enceladus’ south pole, a new mission to Enceladus could search for evidence of life without having to descend through kilometers of ice. In this article, we outline the science motivations for such a successor to Cassini , choosing the primary science goal to be determining whether Enceladus is inhabited and assuming a resource level equivalent to NASA's Flagship-class missions. We selected a set of potential biosignature measurements that are complementary and orthogonal to build a robust case for any life detection result. This result would be further informed by quantifications of the habitability of the environment through geochemical and geophysical investigations into the ocean and ice shell crust. This study demonstrates that Enceladus’ plume offers an unparalleled opportunity for in situ exploration of an Ocean World and that the planetary science and astrobiology community is well equipped to take full advantage of it in the coming decades.

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ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations

Abstract: Abstract

Cited by 6 publications

References 16 publications

ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations – Field Demonstration

ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations – Field Demonstration

Onboard Science Instrument Autonomy for the Detection of Microscopy Biosignatures on the Ocean Worlds Life Surveyor

Science Objectives for Flagship-Class Mission Concepts for the Search for Evidence of Life at Enceladus

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