Elucidating multifaceted urease producing marine Pseudomonas aeruginosa BG as a cogent PGPR and bio-control agent

Abstract

Species belonging to the genus of Pseudomonas are known to possess metabolic versatility and are capable of adapting to various environments. One such strain BG, possessing urease activity, was isolated from marine water from the Gulf of Khambhat, Gujarat. 16S rRNA gene sequence analysis was performed to identify the isolate, which showed that it was the closest neighbor to be Pseudomonas aeruginosa strain BS8. Strain BG was accessed (meant to be assessed?) for multiple plant growth promoting and biocontrol traits. It tested positive for indole-3-acetic acid (IAA) production with 19 mg ml -1 of IAA yield, phosphate solubilization with 13 mg ml -1 solubilization of tri-calcium-phosphate and it showed maximum of 27 mg ml -1 of ammonia production. Further, BG isolate could produce hydrocyanic acid, siderophore, catalase and showed growth inhibition of Aspergillus flavus and Fusarium oxysporum f. sp. cubense confirming its potential antifungal activity. The marine isolate also showed 17.92 ± 1.79 unit min -1 ml -1 urease activity, and further, the presence of genes responsible for urease enzyme in strain BG was identified by amplification using gene specific primers. Talc based biofertilizer using strain BG was prepared and tested on seedling growth of Chickpea, where biofertilizer treated seeds showed enhanced growth. Thus, it was concluded that marine P. aeruginosa BG showed plant growth promotion and biocontrol abilities along with urease activity.

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