Elongation index of erythrocytes, study of activity of chosen erythrocyte enzymes, and the levels of glutathione, malonyldialdehyde in polycythemia vera (PV)

Dabrowski, Z; Dybowicz, A.J.; Marchewka, Anna; Teległów, Aneta; Skotnicki, Aleksander B.; Zduńczyk, A; Aleksander, Paulina Elzbieta; Filar-Mierzwa, Katarzyna

doi:10.3233/ch-2010-1368

Cited by 6 publications

(5 citation statements)

References 6 publications

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“…Our previously published data (Lo et al, 2012), in agreement with other authors (Dabrowski et al, 2011), suggest that hemorheological determinants may affect the pathophysiology of the hyperviscosity syndrome which occurs with polycythaemia vera.…”

Section: Polycythaemia Verasupporting

confidence: 88%

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Reflections on the unexpected laboratory finding of hemorheological alterations observed in some haematological disorders

Caimi

Presti

Carlisi

2021

Microvascular Research

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Section: Polycythaemia Verasupporting

confidence: 88%

“…Other authors (Dabrowski et al, 2011) have found a significant decrease of erythrocyte deformability in patients with PV, which has been associated to the increased concentration of glutathione and malonyl dialdehyde.…”

Section: Polycythaemia Veramentioning

confidence: 82%

Reflections on the unexpected laboratory finding of hemorheological alterations observed in some haematological disorders

Caimi

Presti

Carlisi

2021

Microvascular Research

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“…Interestingly, an increase in erythrocyte AChE activity was detected among patients suffering from polycythemia vera, a myeloproliferative blood cancer in which bone marrow makes too many red blood cells (Dąbrowski et al . ). These findings suggest that in hematopoietic cells, AChE is expressed in a cell‐lineage specific manner, and which may have a possible role in regulating erythroid growth and differentiation.…”

Section: Discussionmentioning

confidence: 97%

Erythropoietin regulates the expression of dimeric form of acetylcholinesterase during differentiation of erythroblast

Luk

et al. 2018

Journal of Neurochemistry

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Acetylcholinesterase (AChE; EC 3.1.1.7) is known to hydrolyze acetylcholine at cholinergic synapses. In mammalian erythrocyte, AChE exists as a dimer (G ) and is proposed to play role in erythropoiesis. To reveal the regulation of AChE during differentiation of erythroblast, erythroblast-like cells (TF-1) were induced to differentiate by application of erythropoietin (EPO). The expression of AChE was increased in parallel to the stages of differentiation. Application of EPO in cultured TF-1 cells induced transcriptional activity of ACHE gene, as well as its protein product. This EPO-induced event was in parallel with erythrocytic proteins, for example, α- and β-globins. The EPO-induced AChE expression was mediated by phosphorylations of Akt and GATA-1; because the application of Akt kinase inhibitor blocked the gene activation. Erythroid transcription factor also known as GATA-1, a downstream transcription factor of EPO signaling, was proposed here to account for regulation of AChE in TF-1 cell. A binding sequence of GATA-1 was identified in ACHE gene promoter, which was further confirmed by chromatin immunoprecipitation (ChIP) assay. Over-expression of GATA-1 in TF-1 cultures induced AChE expression, as well as activity of ACHE promoter tagged with luciferase gene (pAChE-Luc). The deletion of GATA-1 sequence on the ACHE promoter, pAChE -Luc, reduced the promoter activity during erythroblastic differentiation. On the contrary, the knock-down of AChE in TF-1 cultures could lead to a reduction in EPO-induced expression of erythrocytic proteins. These findings indicated specific regulation of AChE during maturation of erythroblast, which provided an insight into elucidating possible mechanisms in regulating erythropoiesis.

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“…In addition to Lu/BCAM, PV RBCs show the abnormal expression of several proteins, including proteins from the endoplasmic reticulum, such as calreticulin and calnexin, and transporters, like ATP binding cassette Subfamily G member 2 (ABCG2) [36], that could directly or indirectly affect cell surface properties. Moreover, elevated values of malonyldialdehyde associated with lipid peroxidation and reactive oxygen species (ROS) production were described in PV RBCs [32] (Figure 2). Such high values in a PV context may promote RBC adhesion to endothelial cells as ROS production is known to increase the expression of cell adhesion molecules intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) on the vascular endothelium through NF-kB activation [46] (Figure 2).…”

Section: Enhanced Rbc Adhesion To the Vascular Endotheliummentioning

confidence: 98%

“…Investigations of PV RBC morphodynamics using laser-assisted approaches have shown a significant reduction in PV RBC deformability [31]. D ąbrowski and colleagues showed an elevated activity of glucose-6-phosphate dehydrogenase and acetylcholinesterase in PV RBCs that together with elevated levels of glutathione and malonyldialdehyde can explain their increased rigidity [32] (Figure 2). Ektacytometry experiments using LoRRca (laser-optical rotational red cell analyzer), confirmed the reduced deformability and elasticity of PV RBCs and showed increased aggregation amplitude compared to those from heathy donors [31].…”

Section: Altered Deformabilitymentioning

confidence: 99%

Red Blood Cell Contribution to Thrombosis in Polycythemia Vera and Essential Thrombocythemia

Grenier,

El Nemer,

De Grandis

2024

IJMS

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Polycythemia vera (PV) and essential thrombocythemia (ET) are myeloproliferative neoplasms (MPN) characterized by clonal erythrocytosis and thrombocytosis, respectively. The main goal of therapy in PV and ET is to prevent thrombohemorrhagic complications. Despite a debated notion that red blood cells (RBCs) play a passive and minor role in thrombosis, there has been increasing evidence over the past decades that RBCs may play a biological and clinical role in PV and ET pathophysiology. This review summarizes the main mechanisms that suggest the involvement of PV and ET RBCs in thrombosis, including quantitative and qualitative RBC abnormalities reported in these pathologies. Among these abnormalities, we discuss increased RBC counts and hematocrit, that modulate blood rheology by increasing viscosity, as well as qualitative changes, such as deformability, aggregation, expression of adhesion proteins and phosphatidylserine and release of extracellular microvesicles. While the direct relationship between a high red cell count and thrombosis is well-known, the intrinsic defects of RBCs from PV and ET patients are new contributors that need to be investigated in depth in order to elucidate their role and pave the way for new therapeutical strategies.

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Elongation index of erythrocytes, study of activity of chosen erythrocyte enzymes, and the levels of glutathione, malonyldialdehyde in polycythemia vera (PV)

Cited by 6 publications

References 6 publications

Reflections on the unexpected laboratory finding of hemorheological alterations observed in some haematological disorders

Reflections on the unexpected laboratory finding of hemorheological alterations observed in some haematological disorders

Erythropoietin regulates the expression of dimeric form of acetylcholinesterase during differentiation of erythroblast

Red Blood Cell Contribution to Thrombosis in Polycythemia Vera and Essential Thrombocythemia

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