Ellagic Acid Suppresses Lipid Accumulation by Suppressing Early Adipogenic Events and Cell Cycle Arrest

Abstract

Ellagic acid (EA) is a natural polyphenol found in various fruits and vegetables. In this study, we examined the inhibitory effect of EA on fat accumulation in 3T3-L1 cells during adipogenesis. Our data showed that EA reduced fat accumulation by down-regulating adipogenic markers such as peroxisome proliferator activated receptor γ (PPARγ) and the CCAAT/enhancer binding protein α (C/EBPα) at the mRNA and protein levels in a dose-dependent manner. We found that the decrease in adipogenic markers resulted from reduced expression of some early adipogenic transcription factors such as KLF4, KLF5, Krox20, and C/EBPβ within 24 h. Also, these inhibitions were correlated with down-regulation of TG synthetic enzymes, causing inhibition of triglyceride (TG) levels in 3T3-L1 cells investigated by ORO staining and in zebrafish investigated by TG assay. Additionally, the cell cycle analysis showed that EA inhibited cell cycle progression by arresting cells at the G0/G1 phase.

Bioactivity-guided isolation and identification of anti-adipogenic compounds from Sanguisorba officinalis

“…These results are in good agreement with a previous study by Woo et al. ( 2015 ), in which ellagic acid inhibits adipocyte differentiation by suppressing early adipogenic events and cell cycle arrest. Furthermore, Lee et al.…”

Caffeic acid and hydroxytyrosol have anti-obesogenic properties in zebrafish and rainbow trout models

“…The protocol consisted of a 15-day exposure to the compound on a high-fat diet background, starting at the first feeding period. For example, curcumin [50–53], indole-3-carbinol [54], baicalein [55], dieckol [56], ellagic acid [52], kaempferol [51], quercetin [56], seapolynol [57] and silibinin [58], have all been reported to inhibit lipid accumulation in these early, post-embryonic developmental stages, when the adipocyte lineage is just starting to be established [20,27]. In our work, we used the ZOT protocol at later larval developmental stages, when the animals had a well-established WAT [30].…”

Euphorbia supina extract results in inhibition of high‑fat‑diet‑induced obesity in mice

“…Many studies have reported the anti-inflammatory, anti-cancer and hepatoprotective effects of corilagin (35)(36)(37). Previous studies have also reported that ellagic acid improved hepatic steatosis and suppressed serum resistin levels in obese mice (38,39), and that ellagic acid suppresses lipid accumulation by inhibiting adipogenesis and cell proliferation (40). In a previous study, it was demonstrated that the anti-obesity effect of Geranium thunbergii was due to the presence of components including corilagin and ellagic acid (41).…”