Elite genotypes/chemotypes, with high contents of madecassoside and asiaticoside, from sixty accessions of Centella asiatica of south India and the Andaman Islands: For cultivation and utility in cosmetic and herbal drug applications

Thomas, M. T.; Kurup, Rajani; Johnson, Anil John; Chandrika, Sreeja Purushothaman; Mathew, Paravanparampil Jacob; Dan, Mathew; Baby, Sabulal

doi:10.1016/j.indcrop.2010.07.003

Cited by 59 publications

(39 citation statements)

References 17 publications

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“…% residual standard deviations (RSD) were determined as 2.63 (L-dopa) and 3.58 (SDP). Limit of detection (LOD, average of 3.3 × SD of peak area/slope of calibration curve for 56 accessions, n = 6) and limit of quantification (LOQ, average of 10 × SD of peak area/slope of calibration curve for 56 accessions, n = 6) were determined for both L-dopa (LOD −31.46 ng, LOQ −95.32 ng) and SDP (LOD −21.10 ng, LOQ −63.93 ng) 48 49 .…”

Section: Methodsmentioning

confidence: 99%

Levodopa in Mucuna pruriens and its degradation

Pulikkalpura

Kurup

Mathew

et al. 2015

Sci Rep

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Mucuna pruriens is the best known natural source of L-dopa, the gold standard for treatment of Parkinsonism. M. pruriens varieties are protein rich supplements, and are used as food and fodder worldwide. Here, we report L-dopa contents in seeds of fifty six accessions of four M. pruriens varieties, M. pruriens var. pruriens, M. pruriens var. hirsuta, M. pruriens var. utilis and M. pruriens var. thekkadiensis, quantified by HPTLC-densitometry. L-dopa contents varied between 0.58 to 6.42 (%, dr. wt.). High and low L-dopa yielding genotypes/chemotypes of M. pruriens could be multiplied for medicinal and nutritional purposes, respectively. HPTLC profiles of M. pruriens seeds on repeated extraction (24 h) in 1:1 formic acid-alcohol followed by development in butanol:acetic acid:water (4:1:1, v/v) showed consistent degradation of L-dopa (Rf 0.34 ± 0.02) into a second peak (Rf 0.41 ± 0.02). An average of 52.11% degradation of L-dopa was found in seeds of M. pruriens varieties. Since M. pruriens seeds and/or L-dopa are used for treatment of Parkinson’s disease and as an aphrodisiac both in modern and/or traditional systems of medicine, the finding of high level of L-dopa degradation (in pure form and in M. pruriens extracts) into damaging quinones and ROS is very significant.

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Section: Methodsmentioning

confidence: 99%

Levodopa in Mucuna pruriens and its degradation

Pulikkalpura

Kurup

Mathew

et al. 2015

Sci Rep

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“…Between the two Centella species, C. asiatica and C. glabrata , the triterpenoid saponin asiaticoside and related compounds are major constituents in the former species and absent in the latter (Long et al ., 2012 ). Similarly, a systematic analysis of the triterpene glycosides asiaticoside and madecassoside in sixty C. asiatica accessions from South India grown under identical environmental conditions revealed huge variations in the content of these saponins, with three accessions having no detectable asiaticoside/madecassoside (Thomas et al ., 2010 ). Analysis of the saponin composition of 800 cultivated ( Glycine max ) and 329 wild ( Glycine soja ) soybean seeds showed clear variety-specific differences between the accessions.…”

Section: Occurrence and Distribution In Plantsmentioning

confidence: 99%

Metabolic and functional diversity of saponins, biosynthetic intermediates and semi-synthetic derivatives

Moses

Papadopoulou

Osbourn

2014

Critical Reviews in Biochemistry and Molecular Biology

382

282

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Saponins are widely distributed plant natural products with vast structural and functional diversity. They are typically composed of a hydrophobic aglycone, which is extensively decorated with functional groups prior to the addition of hydrophilic sugar moieties, to result in surface-active amphipathic compounds. The saponins are broadly classified as triterpenoids, steroids or steroidal glycoalkaloids, based on the aglycone structure from which they are derived. The saponins and their biosynthetic intermediates display a variety of biological activities of interest to the pharmaceutical, cosmetic and food sectors. Although their relevance in industrial applications has long been recognized, their role in plants is underexplored. Recent research on modulating native pathway flux in saponin biosynthesis has demonstrated the roles of saponins and their biosynthetic intermediates in plant growth and development. Here, we review the literature on the effects of these molecules on plant physiology, which collectively implicate them in plant primary processes. The industrial uses and potential of saponins are discussed with respect to structure and activity, highlighting the undoubted value of these molecules as therapeutics.

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“…After quantifying the asiaticosides, 7 accessions have asiaticoside greater than 1%, another 7 have asiaticoside less than 0.1%, 3 accessions have non-detectable asiaticoside; in the madecassoside, 5 accessions have madecassoside greater than 4%, another 8 accessions have madecassoside between 3 -3.99%, 15 accessions have madecassoside less than 1% while 2 accessions have the lowest value of madecassoside, an accession 53/Ca 053 from Nanora in North Goa didn't show both asiaticoside and madecassoside (Thomas et al, 2010). The results show that genetical factors play an important role in the biosynthesis of the triterpenes in C. asiatica (Thomas et al, 2010).…”

Section: International Accessionmentioning

confidence: 97%

“…Study on 60 different accession from South India and Andaman Island indicated that when the accessions were planted and collected under same location and conditions; when the environmental influences has been nullified and differences can only be genetically determined, there was still variation in the chemical profiling of asiaticoside and madecassoside (Thomas et al, 2010). After quantifying the asiaticosides, 7 accessions have asiaticoside greater than 1%, another 7 have asiaticoside less than 0.1%, 3 accessions have non-detectable asiaticoside; in the madecassoside, 5 accessions have madecassoside greater than 4%, another 8 accessions have madecassoside between 3 -3.99%, 15 accessions have madecassoside less than 1% while 2 accessions have the lowest value of madecassoside, an accession 53/Ca 053 from Nanora in North Goa didn't show both asiaticoside and madecassoside (Thomas et al, 2010).…”

Section: International Accessionmentioning

confidence: 99%

Accession in Centella asiatica; Current Understanding and Future Knowledge

Gbolahan¹,

Abiola²,

Kamaldin³

et al. 2016

J PURE APPL MICROBIOL

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Centella asiatica is described as a potential cure-all because of its wide usage. The accessions in Centella asiatica make standardisation important before using it. In Malaysia alone, there are more than 15 accessions of Centella asiatica with each having variation in their secondary metabolites. There are several reports from India to Korea, from Madagascar to Malaysia and from Thailand to South Africa about variations in chemical profiling of secondary metabolites and the reasons for these variations in Centella asiatica. Despite all these reports, there has been no attempt to bring all these variations together and discuss them under one heading resulting into scattered information about accession in Centella asiatica. In this review, we discuss the impact of accessions in Centella asiatica and what more need to be done to our knowledge of accession in Centella asiatica.

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Elite genotypes/chemotypes, with high contents of madecassoside and asiaticoside, from sixty accessions of Centella asiatica of south India and the Andaman Islands: For cultivation and utility in cosmetic and herbal drug applications

Cited by 59 publications

References 17 publications

Levodopa in Mucuna pruriens and its degradation

Levodopa in Mucuna pruriens and its degradation

Metabolic and functional diversity of saponins, biosynthetic intermediates and semi-synthetic derivatives

Accession in Centella asiatica; Current Understanding and Future Knowledge

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