Elicitation of Stevia Glycosides Using Salicylic Acid and Silver Nanoparticles Under Callus Culture

Golkar, Pooran; Moradi, Mahsa; Garousi, Ghasem Ali

doi:10.1007/s12355-018-0655-6

Cited by 57 publications

(32 citation statements)

References 31 publications

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“…They determined the stevioside and rebaudioside A production as 42 mg/g DW and 10.50 mg/g DW, respectively. Golkar et al [28] enhanced the stevioside and rebaudioside A concentrations up to 32.34 mg/g DW and 3.40 mg/g DW in callus culture exposed to 45 mg/L silver nanoparticles (Ag NPs) and 0.25 mg/L SA, respectively. Javed et al [29] cultured the nodal explants of S. rebaudiana on the media containing different concentrations of Copper oxide (CuO) nanoparticles (NPs) and determined the highest stevioside and rebaudioside A production as 2.06% and 4.17% in the shoots exposed to 10 mg/L CuO NPs, respectively.…”

Section: Steviol Glycosides Productionmentioning

confidence: 99%

Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana

Bayraktar

Naziri

Karabey

et al. 2018

International Journal of Secondary Metabolite

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Stevia rebaudiana Bertoni, which is an important plant for the food and health sector, contains calorie-free natural sweet-tasting steviol glycosides (SGs). In the present study, the effects of different elicitors [methyl jasmonate (MeJA), salicylic acid (SA), or chitosan (CHI)] on the in vitro production of stevioside and rebaudioside A were carried out. For this purpose, 3-week-old in vitro plantlets were transferred into 250 mL flasks containing liquid woody plant medium (WPM) supplemented with MeJA, SA, or CHI at different concentrations (0, 50, 100, or 200 µM), and were exposed to these elicitors for 2 weeks. A HPLC method was developed to quantify the aforementioned SGs in the cultivated plantlets and all of the elicitor types and concentrations resulted in an increase in stevioside production ranged between 2.87 mg/g dry weight (DW) (Control) and 50.07 mg/g DW (100 µM MeJA). The highest number of shoot, node, leaf, leaf length, and biomass accumulation and shoot length were observed with application of 100 µM CHI and control, respectively. The present findings open new perspectives for increasing the stevioside production using a plant tissue culture system.

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Section: Steviol Glycosides Productionmentioning

confidence: 99%

Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana

Bayraktar

Naziri

Karabey

et al. 2018

International Journal of Secondary Metabolite

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“…The impact of MNPs on SM production was reported to depend on the characteristics of the MNPs (i.e., the concentration used, the exposure time and their size and synthetic origin), but also on the plant culture type. [28][29][30] In this review, we have summarized these different parameters that can affect the impact of MNPs on SM production in a number of in vitro cultures of crops and/ or medicinal plant species.…”

Section: Metallic Nanoparticlesmentioning

confidence: 99%

“…Plant growth regulators have been employed along with different MNPs to check their combinative effect on elicitation of SMs in various plant species. 28,51,52 In combination with plant growth regulators (TDZ and 2,4-D), AgNP elicitation of bitter gourd (Momordica charantia) cell culture resulted in an enhanced production of phenolic, hydroxycinnamic and hydroxybenzoic acids, and avonoids. 53 Enhanced production of stevia glycosides was observed in callus culture of Stevia rebaudiana Bertoni simultaneously elicited with AgNPs and salicylic acid.…”

Section: Metallic Nanoparticlesmentioning

confidence: 99%

Advances in nanomaterials as novel elicitors of pharmacologically active plant specialized metabolites: current status and future outlooks

et al. 2019

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“…These nanoparticles are among the latest currently studied elicitors [ 51 , 52 , 53 ]. It has been proven that metal nanoparticles are highly reactive toward plants.…”

Section: Introductionmentioning

confidence: 99%

“…The favorable influence of elicitation by metal nanoparticles on the production of biologically active compounds in plants has been confirmed by Zhang et al [ 41 , 75 ] for Artemisia anuua , Sharafi et al [ 42 , 76 ] for Hypericum perforatum , and Oloumi et al [ 43 , 77 ] for Glycyrrhiza glabra . Silver and gold nanoparticles have been used as elicitors in cultures of Prunella vulgaris [ 78 ], Stevia glycosides [ 52 ], Cucumis anguria [ 79 ], and Caralluma tuberculata [ 80 ]. The latest scientific reports indicate significant changes in the metabolite profile of Arabidopsis thaliana under the influence of silver nanoparticles [ 81 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of AuNPs and AgNPs on the Antioxidant System and Antioxidant Activity of Lavender (Lavandula angustifolia Mill.) from In Vitro Cultures

et al. 2020

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The aim of this study was to determine the effect of gold and silver nanoparticles on the activity of antioxidant enzymes (ascorbate peroxidase (APX), superoxide dismutase (SOD), guaiacol peroxidase (POX), and catalase (CAT)), the free radical scavenging capacity, and the total polyphenol capacity of lavender (Lavandula angustifolia Mill.) cultivar “Munstead” propagated in vitro. In the experiment, fragments of lavender plants were cultivated in vitro on medium with the addition of 1, 2, 5, 10, 20, and 50 mg∙dm−3 of AgNPs or AuNPs (particle sizes 24.2 ± 2.4 and 27.5 ± 4.8 nm, respectively). It was found that the nanoparticles increase the activity of the antioxidant enzymes APX and SOD; however, the reaction depends on the NP concentration. The highest APX activity is found in plants propagated on media with 2 and 5 mg∙dm−3 of AgNPs. AuNPs significantly increase the APX activity when added to media with a concentration of 10 mg∙dm−3. The highest SOD activity is recorded at 2 and 5 mg∙dm−3 AgNP and AuNP concentrations. The addition of higher concentrations of nanoparticles to culture media results in a decrease in the APX and SOD activity. The addition of AuNPs to culture media at concentrations from 2 to 50 mg∙dm−3 increases the POX activity in comparison to its activity when AgNPs are added to the culture media. No significant influence of NPs on the increase in CAT activity was demonstrated. AgNPs and AuNPs increased the free radical scavenging capacity (ABTS•+). The addition of NPs at concentrations of 2 and 5 mg∙dm−3 increased the production of polyphenols; however, in lower concentrations it decreased their content in lavender tissues.

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Elicitation of Stevia Glycosides Using Salicylic Acid and Silver Nanoparticles Under Callus Culture

Cited by 57 publications

References 31 publications

Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana

Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana

Advances in nanomaterials as novel elicitors of pharmacologically active plant specialized metabolites: current status and future outlooks

Effect of AuNPs and AgNPs on the Antioxidant System and Antioxidant Activity of Lavender (Lavandula angustifolia Mill.) from In Vitro Cultures

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