Elicitation of galanthamine biosynthesis by Leucojum aestivum liquid shoot cultures

Identification, molecular characterization and expression of JAZ genes in Lycoris aurea

“…In Salvia miltiorrhiza hairy roots, JA-induced tanshinone biosynthesis is mediated by JAZ proteins and SmJAZ8 acts as a core repressor [59,60]. Also, it has been reported that the enhanced accumulation of Amaryllidaceae alkaloids can be elicited by addition of exogenous JAs treatment in Amaryllidaceae plants [37,38,62,63], but the exact molecular regulation mechanisms, including those related with JAZs, are still obscure in this plant family. As a key regulator in plant jasmonate signal pathway, JAZ proteins should play important regulatory role in Amaryllidaceae alkaloids biosynthesis.…”

Production of fumaric acid from Fumaria sp. plant in vitro systems

“…Next scale-up of cultivation in different type bioreactors, as well as applied additional approaches for cultivation regime (such as two-phase cultivation) led to the additional improvement of yields of target metabolites by the plant in vitro systems. As successful examples could be noted biosynthesis of rosmarinic acid by Lavandula vera MM cell suspension up to 50 fold increase (Pavlov et al 2005) and biosynthesis of galanthamine by shoots of Leucojum aestivum (1.36 fold increase) (Ivanov et al 2013). Hence, for more detailed assessment of the capacity of F. officinalis cell suspension for development commercial process for fumaric acid production more detailed extensive experimental work should be performed.…”

Galanthamine biosynthesis in plant in vitro systems

“…In that study again it was found that methyl jasmonate increased Gal biosynthesis most strongly. Recent studies in our laboratory have indicated that methyl jasmonate increase mainly the transcription of the enzyme PAL, and to a lesser degree that of tyrosine decarboxylase. On the other hand, jasmonic acid advantageously increases the transcription of tyrosine decarboxylase, and to a lesser extent the transcription of PAL.…”

“…Maximum amounts of Gal are biosynthesized at higher concentrations of the carbon source (C/N-21/1) compared to the standard medium (15/1) [29]. The elevated carbon and nitrogen concentrations expectedly have a positive impact due to the relationship between the carbon source and Shoot RITA 0.05% DW [35] the biosynthesis of secondary metabolites, on the one hand, and the presence of a nitrogen atom in the Gal molecule, on the other [27]. In the optimal medium, the alkaloid profile in the shoot culture also changes.…”