Elevation of Apolipoprotein A-I and Apolipoprotein H Levels in the Vitreous Fluid and Overexpression in the Retina of Diabetic Patients

Simó, Rafael; Higuera, Mónica; Garcia-Ramírez, Marta; Canals, Françesc; García‐Arumí, José; Hernández, Cristina

doi:10.1001/archopht.126.8.1076

Cited by 64 publications

(59 citation statements)

References 38 publications

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“…Simo et al studied vitreous samples from 4 diabetic patients with PDR and 8 nondiabetic patients with macular hole for proteomic analysis of ApoA1 and ApoH [54]. The authors found that intravitreous ApoA1 and ApoH levels were significantly higher in patients with PDR than in the control group.…”

Section: Associations With Nontraditional Lipid Markersmentioning

confidence: 99%

Dyslipidemia and Diabetic Retinopathy

Chang

2013

Rev Diabet Stud

100

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■ AbstractDiabetic retinopathy (DR) is one of the major microvascular complications of diabetes. In developed countries, it is the most common cause of preventable blindness in diabetic adults. Dyslipidemia, a major systemic disorder, is one of the most important risk factors for cardiovascular disease. Patients with diabetes have an increased risk of suffering from dyslipidemia concurrently. The aim of this article is to review the association between diabetic retinopathy (DR) and traditional/nontraditional lipid markers, possible mechanisms involving lipid metabolism and diabetic retinopathy, and the effect of lipid-lowering therapies on diabetic retinopathy. For traditional lipid markers, evidence is available that total cholesterol and low-density lipoprotein cholesterol are associated with the presence of hard exudates in patients with DR. The study of nontraditional lipid markers is advancing only in recently years. The severity of DR is inversely associated with apolipoprotein A1 (ApoA1), whereas ApoB and the ApoB-to-ApoA1 ratio are positively associated with DR. The role of lipid-lowering medication is to work as adjunctive therapy for better control of diabetes-related complications including DR.

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Section: Associations With Nontraditional Lipid Markersmentioning

confidence: 99%

Dyslipidemia and Diabetic Retinopathy

Chang

2013

Rev Diabet Stud

100

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“…29 Apo A 1 is a potent scavenger of oxygen-reactive species, and may have a role in protecting retina from the oxidative stress due to diabetes. 30 Garcia-Ramirez et al identified several components of the complement factor (C4b, factor B, C3 and C9) in PDR vitreous. 31 These authors postulated that activation of the complement cascade leads to initiation and progression of thrombosis, leucostasis and apoptosis, causing the vascular lesions in diabetic retinopathy.…”

Section: The Vitreous Proteome In Diabetic Retinopathymentioning

confidence: 99%

“…The levels of PEDF, a potent inhibitor of angiogenesis, have not been found to be consistent across different studies, some reporting it to be down-regulated 31 ; and others as up-regulated 27,29 in the PDR vitreous. Other proteins that have been found to be increased in abundance in the diabetic vitreous include apo H 30 , prostaglandin –D2 synthetase, plasma glutathione peroxidase, intra retinol binding protein, catalase, 32 enolase, 32 prostaglandin-H2 D isomerase, 33 serine protease inhibitor, 33 ankyrin repeat domain 15 protein, 33 angiotensiogen 27 , prothrombin 27 , antithrombin III 27 , Factor XII 27 and peroxiredoxin-1. 27 …”

Section: The Vitreous Proteome In Diabetic Retinopathymentioning

confidence: 99%

Vitreous Proteomics and Diabetic Retinopathy

Walia

Clermont

Gao

et al. 2010

Seminars in Ophthalmology

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Diabetic retinopathy is the major cause of acquired blindness in working age adults. Studies of the vitreous proteome have provided insights into the etiology of diabetic retinopathy and suggested potential molecular targets for treatments. Further characterization of the protein changes associated with the progression of this disease may suggest additional therapeutic approaches as well as reveal novel factors that may be useful in predicting risk and functional outcomes of interventional therapies. This article provides an overview of the various techniques used for proteomic analysis of the vitreous and details results from studies evaluating vitreous of diabetic patients using the proteomic approach.

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“…However, Apo A-I was recently found in the vitreous fluid and human RPE cells. 23 Apo A-I has a protective effect on the corneal epithelium and may improve the epithelial conditions of the ocular surface. 24 Clusterin is also present in human tears.…”

mentioning

confidence: 99%

Proteomic analysis of tears following acupuncture treatment for menopausal dry eye disease by two-dimensional nano-liquid chromatography coupled with tandem mass spectrometry

Liu¹,

Liu²,

Ren³

et al. 2017

IJN

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Background The purpose of this study was to investigate whether acupuncture is effective at treating dry eye disease among postmenopausal women and to identify the possible mechanisms. Methods Twenty-eight postmenopausal women with dry eye disease were randomly divided into two groups: an acupuncture plus artificial tears (AC + AT) group and an artificial tears (AT) only group. After baseline examination of clinical parameters and tear sample collection, each patient received the designated modality of topical therapy for 2 months. Post-treatment documentation of clinical parameters was recorded, and tear samples were collected. Tear samples from the AC + AT group were subjected to two-dimensional nano-liquid chromatography coupled with tandem mass spectrometry (2D nano-LC-MS/MS). Western blot analysis was also performed on tear samples from both groups. Results After treatment, the Ocular Surface Disease Index scores, symptom assessment scores, scores of sign assessment, and tear break-up time were significantly improved in both groups ( P =0.000). Symptom assessment scores were significantly improved in the AC + AT group ( P =0.000) compared with the AT group. 2D nano-LC-MS/MS identified 2,411 proteins, among which 142 were downregulated and 169 were upregulated. After combined AC + AT treatment, the abundance of secreted proteins was increased, whereas that of cytoplasmic proteins decreased (Pearson’s χ 2 test, P =0.000, P =0.000, respectively). Proteins involved in immunity and regulation were also more abundant (Pearson’s χ 2 test, P =0.040, P =0.016, respectively), while components and proliferation-related proteins were downregulated (Pearson’s χ 2 test, P =0.003, P =0.011, respectively). Conclusion AC + AT treatment increased protein synthesis and secretion, and improved clinical symptoms. These results indicate that acupuncture may be a complimentary therapy for treating postmenopausal dry eye disease.

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Elevation of Apolipoprotein A-I and Apolipoprotein H Levels in the Vitreous Fluid and Overexpression in the Retina of Diabetic Patients

Cited by 64 publications

References 38 publications

Dyslipidemia and Diabetic Retinopathy

Dyslipidemia and Diabetic Retinopathy

Vitreous Proteomics and Diabetic Retinopathy

Proteomic analysis of tears following acupuncture treatment for menopausal dry eye disease by two-dimensional nano-liquid chromatography coupled with tandem mass spectrometry

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