Elevated-temperature ultrahigh-pressure liquid chromatography using very small polybutadiene-coated nonporous zirconia particles

Xiang, Yanqiao; Yan, Bingwen; Yue, Bingfang; McNeff, Clayton V.; Carr, Peter W.; Lee, Milton L.

doi:10.1016/s0021-9673(02)01662-x

Cited by 63 publications

(40 citation statements)

References 24 publications

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“…Furthermore, and as already reported in the introduction, a high temperature decreases the mobile phase viscosity, which reduces the pressure drop and makes the use of longer columns or smaller particles possible. Lee and coworkers [66,67] investigated this approach on a capillary column (50 lm id, 14.5 cm length, 1.0 lm nonporous particles) packed with zirconia material due to its chemical stability at elevated temperatures. They developed a separation of benzodiazepines in less than 1.2 min at 1008C using this column at a pressure of 1480 bar (Fig.…”

Section: Preliminary Work At Very High Pressurementioning

confidence: 99%

Fast analysis in liquid chromatography using small particle size and high pressure

Nguyen

Guillarme

Rudaz

et al. 2006

J of Separation Science

301

164

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ReviewFast analysis in liquid chromatography using small particle size and high pressureIn order to enhance chromatographic performances in terms of efficiency and rapidity, LC has recently evolved in the development of short columns packed with small particles (sub-2 lm) working at high pressures (A400 bar). This approach has been described 30 years ago according to the fundamental chromatographic equations. However, systems and columns compatible with such high pressures have been introduced in the market in 2004 only. Advantages of small particles working at high pressure will be discussed in terms of sensitivity, efficiency, resolution, and analysis time. Potential problems encountered with high pressure in terms of frictional heating and solvent compressibility will also be discussed even if systems working at a maximum pressure of 1000 bar are not influenced by these parameters and give reliable and reproducible results. Several applications will highlight the potential and interest of this new technology.

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Section: Preliminary Work At Very High Pressurementioning

confidence: 99%

Fast analysis in liquid chromatography using small particle size and high pressure

Nguyen

Guillarme

Rudaz

et al. 2006

J of Separation Science

301

164

View full text Add to dashboard Cite

show abstract

“…Although one can decrease t G and maintain resolution by using smaller particles [13], the pressure limitation of conventional instrumentation (~ 40 MPa) sets the upper limit of speed. To solve this problem, Jorgenson and coworkers [14][15][16], and Lee and coworkers [17][18][19][20] have studied ultra-high pressure liquid chromatography with instrumentation that can withstand significantly higher backpressures.…”

Section: Introductionmentioning

confidence: 99%

High-speed gradient elution reversed-phase liquid chromatography of bases in buffered eluents

Schellinger

Stoll

Carr

2008

Journal of Chromatography A

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We studied the run-to-run repeatability of the retention times of both non-ionizable and basic compounds chromatographed using buffered eluents. The effect of flow rate, organic modifier and other additives, buffer type/concentration, stationary phase type, batch-to-batch preparation of the initial eluent, gradient time, sample type and intra-day changes on retention repeatability were examined. We also assessed the effect of column storage solvent conditions on the inter-day repeatability. Although retention repeatability is strongly influenced by many parameters (flow rate, solvent compressibility compensation, precision of temperature control, and buffer/stationary phase type), our primary finding is that with a reasonable size column (15 cm by 4.6 mm (i.d.)) two column volumes of reequilibration with initial eluent suffices to provide acceptable repeatability (no worse than 0.004 min) for both non-ionizable and basic analytes under a wide variety of conditions. Under ideal conditions (e.g. the right buffer, flow rate, etc.) it is possible to obtain truly extraordinary repeatability often as good as 0.0004 minutes. These absolute fluctuations in retention translate to worst case changes in resolution of 0.2 units and average changes of only 0.02 units.

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“…Literature on available stationary phases for high temperature and temperature-programmed LC is available [10,12,13,[15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31]. In 2004, a special issue of Journal of Chromatography A [32] was published on developments in stationary phases and retention, including phases for high temperature.…”

Section: Introductionmentioning

confidence: 99%

“…About 100 hits were obtained of which more than half discuss applications, and only about 20% of them cover temperature programming. The applications involve polymers and surfactants [34][35][36][37][38][39][40][41][42], natural products [43][44][45][46][47][48][49][50][51][52][53], pharmaceuticals [54][55][56][57][58], environmental analyses [31,[59][60][61][62][63], ion chromatography [64][65][66][67], proteins [30,68,69], and peptides [70][71][72][73][74][75], nucleotides [76], denaturing HPLC (DHPLC) [77,78], and chiral analyses [79][80]…”

Section: Introductionmentioning

confidence: 99%

Elevated temperature and temperature programming in conventional liquid chromatography – fundamentals and applications

Vanhoenacker

Sandra²

2006

J of Separation Science

101

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Temperature, as a powerful variable in conventional LC is discussed from a fundamental point of view and illustrated with applications from the author's laboratory. Emphasis is given to the influence of temperature on speed, selectivity, efficiency, detectability, and mobile phase composition (green chromatography). The problems accompanying the use of elevated temperature and temperature programming in LC are reviewed and solutions are described. The available stationary phases for high temperature operation are summarized and a brief overview of recent applications reported in the literature is given.

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Elevated-temperature ultrahigh-pressure liquid chromatography using very small polybutadiene-coated nonporous zirconia particles

Cited by 63 publications

References 24 publications

Fast analysis in liquid chromatography using small particle size and high pressure

Fast analysis in liquid chromatography using small particle size and high pressure

High-speed gradient elution reversed-phase liquid chromatography of bases in buffered eluents

Elevated temperature and temperature programming in conventional liquid chromatography – fundamentals and applications

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