Elevated Serum Total IgE Alter the Diagnostic Performance of Radio-Immune Assays for β-Lactam Specific Antibody Dosing

The Performance of Flowcytometry-Assisted Basophil Activation Tests in the Diagnosis of Drug-Induced Immediate- Type Hypersensitivity Reactions

“…They are the most widely used in vitro tests to confirm drug hypersensitivity retrospectively. For β-lactam antibiotics, the results of the tests are altered by the value of the total serum IgE [5], while for NMBAs, specific IgE antibodies were identified in the serum of normal healthy controls, with no clinical reaction upon exposure [6,40,65].…”

“…However, the performance of such tests varied in previous reports and the accuracy is not 100%. Moreover, serum total IgE influences the results of the assays for βlactam-specific antibody dosing [5]. For the neuromuscular blocking agents (NMBAs), previous studies reported that healthy controls might have uneventful exposures despite positive-specific IgE for muscle relaxants [6,7].…”

Solid phase extraction technology combined with UPLC-MS/MS: a method for detecting 20 β-lactamase antibiotics traces in goat's milk

“…With the extensive application of β-lactam antibiotics and the in-depth research on metabolic mechanism, toxicology and residues, countries have become increasingly strict on residue limits, resulting in research on detection methods. 15,21–24 The detection methods of β-lactam drugs include radio-immunoassay, 25 microbial inhibition, 26 colloidal gold method, 27 and liquid chromatography. 28 These methods have shortcomings such as low sensitivity, poor specificity, and inability to simultaneously confirm qualitative and quantitative detection.…”