Elevated Phosphate Activates N-ras and Promotes Cell Transformation and Skin Tumorigenesis

Camalier, Corinne E.; Young, Matthew R.; Bobe, Gerd; Perella, Christine M.; Colburn, Nancy H.; Beck, George R.

doi:10.1158/1940-6207.capr-09-0068

Cited by 84 publications

(83 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RAS genes are related to cellular responses such as proliferation, cell cycle and apoptosis (Malumbres and Pellicer, 1998). Moreover, in a mouse model of skin carcinogenesis, high doses of dietary phosphate (1.2%) significantly enhanced the skin papilloma number through an increase in the circulating OPN levels (Camalier et al, 2010).…”

Section: Influence Of Pi On Cellular Signaling and Tumorigenesismentioning

confidence: 96%

See 1 more Smart Citation

Biological effects of inorganic phosphate: potential signal of toxicity

et al. 2015

View full text Add to dashboard Cite

-Inorganic phosphate (Pi) plays crucial roles in several biological processes and signaling pathways. Pi uptake is regulated by sodium-dependent phosphate (Na/Pi) transporters (NPTs). Moreover, Pi is used as a food additive in food items such as sausages, crackers, dairy products, and beverages. However, the high serum concentration of phosphate (> 5.5 mg/dL) can cause adverse renal effects, cardiovascular effects including vascular or valvular calcification, and stimulate bone resorption. In addition, Pi can also alter vital cellular signaling, related to cell growth and cap-dependent protein translation. Moreover, intake of dietary Pi, whether high (1.0%) or low (0.1%), affects organs in developing mice, and is related to tumorigenesis in mice. The recommended dietary allowance (RDA) of Pi is the daily dietary intake required to maintain levels above the lower limit of the range of normal serum Pi concentration (2.7 mg/dL) for most individuals (97-98%). Thus, adequate intake of Pi (RDA; 700 mg/day) and maintenance of normal Pi concentration (2.7-4.5 mg/dL) are important for health and prevention of diseases caused by inadequate Pi intake.

show abstract

Section: Influence Of Pi On Cellular Signaling and Tumorigenesismentioning

confidence: 96%

“…Increased levels of Pi also activate signaling pathways involving the neuroblastoma RAS viral oncogene homolog (N-ras) (Camalier et al, 2010). Ras genes are oncogenes that are activated in cancer.…”

Section: Influence Of Pi On Cellular Signaling and Tumorigenesismentioning

confidence: 99%

Biological effects of inorganic phosphate: potential signal of toxicity

et al. 2015

View full text Add to dashboard Cite

show abstract

“…These results agreed with Brada el al [46] and disagreed with Kristensen et al [47] as survival was found to be inversely associated with serum ionized calcium levels. It was found that elevated levels of phosphorus were necessary for adequate growth of some types of cancers [6], [7]. This rise in phosphorus is due to heightened requirements of protein synthesis needed during accelerated cell growth [8].…”

Section: Discussionmentioning

confidence: 99%

“…Elevated levels of serum Pi were found to modify gene expression as well as protein translation and affect the rate of cell proliferation in vitro [4], [5]. Moreover, a high Pi in a diet has been reported to result in a significantly increased development of lung and skin cancers, as well as perturbed normal brain growth in animal studies [6], [7], which denoted the potential link between Pi and carcinogenesis in humans. In the human body, Pi is known to be mainly regulated by a set of hormonal and metabolic factors, which tightly control calcium homeostasis, i.e.…”

Section: Introductionmentioning

confidence: 97%

The role of serum 25-hydroxyvitamin D, ionized calcium and phosphorus in breast cancer females: A case-control study

Moneim¹,

Hewala

Rashad³

et al. 2015

IJBAS

View full text Add to dashboard Cite

Aim: To evaluate the diagnostic and prognostic value of serum 25-hydroxyvitamin D (25(OH) D), ionized calcium and phosphorus in comparison with serum CA15.3 as the most commonly used breast cancer marker. Participants and methods: This study was conducted on 45 breast cancer female patients with recently detected breast cancer before surgery and 45 apparently healthy female controls of matched age, menstrual and socioeconomic status as breast cancer patients group. Serum 25(OH) D, ionized calcium, phosphorus and CA15.3 were measured using readyfor-use commercially available kits. Results: Serum levels of 25(OH) D and ionized calcium in the breast cancer patients group were significantly lower than those of the control group, while serum levels of phosphorus and CA15.3 in breast cancer patients group were significantly higher than those of the control group. The area under the ROC curve for serum ionized calcium (81.7%) was significantly greater than that of 25(OH) D (75.3%), CA 15.3 (70.1%) and phosphorus (62.8%). The odd's ratio of vitamin D was 0.0937 (95% CI=0.0311-0.2823), of ionized calcium was 0.0464 (95% CI=0.015-0.141) and of phosphorus was 2.6801(95% CI=1.1269-6.3742) in breast cancer patients group. Serum phosphorus was significantly correlated with age and menopausal status of breast cancer patients. Conclusion: Our results suggest that serum ionized calcium and 25(OH) D were superior to serum CA15.3 and phosphorus for prediction of breast cancer. In addition, our results indicate that 25 (OH) D and calcium may decrease the risk for breast cancer incidence, while phosphorus may increase this risk. None of the assayed biomarkers has a prognostic role in breast cancer.

show abstract

“…A study by Camalier et al (2010) investigated the effects of evaluated levels of inorganic phosphate on cell proliferation and anchorage-independent growth of transformed epidermal cell line JB6 and mice skin tumorigenesis. Their results indicated that an increase in the level of P i in the cell culture medium promoted cell proliferation and skin tumorigenesis by, in part, increasing the availability of phosphate for activation of N-ras and its downstream targets, ERK1/2, activator protein-1, as well as increased gene expression of Fra-1, COX-2, and osteopontin in a dosedependent manner.…”

Section: Sponge Cell Exposure To Inorganic Phosphatementioning

confidence: 99%

Efforts to develop a cultured sponge cell line: revisiting an intractable problem

Grasela

Pomponi

Rinkevich

et al. 2011

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

Residents of the marine environment, sponges (Porifera) have the ability to produce organic compounds known as secondary metabolites, which are not directly involved in the normal growth, development, or reproduction of an organism. Because of their sessile nature, the production of these bioactive compounds has been interpreted as a functional adaptation to serve in an important survival role as a means to counter various environmental stress factors such as predation, overgrowth by fouling organisms, or competition for limited space. Regardless of the reasons for this adaptation, a variety of isolated compounds have already proven to demonstrate remarkable anticancer, fungicidal, and antibiotic properties. A major obstacle to the isolation and production of novel compounds from sponges is the lack of a large, reliable source of sponge material. Sponge collection from the sea would be environmentally detrimental to the already stressed and sparse sponge populations. Sponge production in an aquaculture setting might appear to be an ideal alternative but would also be cost-ineffective and sponge growth is extremely slow. A third approach involves the development of a sponge cell culture system capable of producing the necessary cell numbers to harvest for research purposes as well as for the eventual commercial-scale production of promising bioactive compounds. Unfortunately, little progress has been made in this direction other than the establishment of temporary cultures containing aggregates and fragments of cells. One impediment toward successful sponge cell culture might be ascribed to a lack of published knowledge of failed methodologies, and thus, time and effort is wasted on continued reinvention of the same methods and procedures. Consequently, we have undertaken here to chart some of our unsuccessful research efforts, our methodology, and results to provide the sponge research community with knowledge to assist them to better avoid taking the same failed pathways.

show abstract

Elevated Phosphate Activates N-ras and Promotes Cell Transformation and Skin Tumorigenesis

Cited by 84 publications

References 60 publications

Biological effects of inorganic phosphate: potential signal of toxicity

Biological effects of inorganic phosphate: potential signal of toxicity

The role of serum 25-hydroxyvitamin D, ionized calcium and phosphorus in breast cancer females: A case-control study

Efforts to develop a cultured sponge cell line: revisiting an intractable problem

Contact Info

Product

Resources

About