Elevated extracellular calcium increases fibroblast growth factor-2 gene and protein expression levels via a cAMP/PKA dependent pathway in cementoblasts

Kanaya, Sousuke; Nemoto, Eiji; Ebe, Yukari; Somerman, Martha J.; Shimauchi, Hidetoshi

doi:10.1016/j.bone.2010.05.042

Cited by 29 publications

(37 citation statements)

References 46 publications

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“…Thus, these cells may also react with changes in the concentrations of extracellular inorganic ions. Whether cementoblasts could sense extracellular Ca 2+ and PO 4 2- ionic concentrations and altered cell functions such as cementogenesis and cytokine production still remains unclear [37]. Increases in extracellular Ca 2+ produced an augmentation in fibroblast growth factor-2 (FGF-2) levels.…”

Section: Discussionmentioning

confidence: 99%

Bioactive Polymeric Nanoparticles for Periodontal Therapy

et al. 2016

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Aimsto design calcium and zinc-loaded bioactive and cytocompatible nanoparticles for the treatment of periodontal disease.MethodsPolymP-nActive nanoparticles were zinc or calcium loaded. Biomimetic calcium phosphate precipitation on polymeric particles was assessed after 7 days immersion in simulated body fluid, by scanning electron microscopy attached to an energy dispersive analysis system. Amorphous mineral deposition was probed by X-ray diffraction. Cell viability analysis was performed using oral mucosa fibroblasts by: 1) quantifying the liberated deoxyribonucleic acid from dead cells, 2) detecting the amount of lactate dehydrogenase enzyme released by cells with damaged membranes, and 3) by examining the cytoplasmic esterase function and cell membranes integrity with a fluorescence-based method using the Live/Dead commercial kit. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests.ResultsPrecipitation of calcium and phosphate on the nanoparticles surfaces was observed in calcium-loaded nanoparticles. Non-loaded nanoparticles were found to be non-toxic in all the assays, calcium and zinc-loaded particles presented a dose dependent but very low cytotoxic effect.ConclusionsThe ability of calcium-loaded nanoparticles to promote precipitation of calcium phosphate deposits, together with their observed non-toxicity may offer new strategies for periodontal disease treatment.

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Section: Discussionmentioning

confidence: 99%

Bioactive Polymeric Nanoparticles for Periodontal Therapy

et al. 2016

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“…It has been established that high level of [Ca 2+ ] o promoted the proliferation in a number of osteoblastic cell lines. A few papers reported that multiple intracellular signal pathways such as calcium/calmodulin, MEK/ERK could be activated by high [Ca 2+ ] o stimulation and played roles in mediating high [Ca 2+ ] o -promoted proliferation in different types of osteoblasts [9], [10], [48]–[51]. But how activation of these signal pathways leads to osteoblastic proliferation is still unclear now.…”

Section: Discussionmentioning

confidence: 99%

Elevation of Extracellular Ca2+ Induces Store-Operated Calcium Entry via Calcium-Sensing Receptors: A Pathway Contributes to the Proliferation of Osteoblasts

Pan

Zhang

et al. 2014

PLoS ONE

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AimsThe local concentration of extracellular Ca2+ ([Ca2+]o) in bone microenvironment is accumulated during bone remodeling. In the present study we investigated whether elevating [Ca2+]o induced store-operated calcium entry (SOCE) in primary rat calvarial osteoblasts and further examined the contribution of elevating [Ca2+]o to osteoblastic proliferation.MethodsCytosolic Ca2+ concentration ([Ca2+]c) of primary cultured rat osteoblasts was detected by fluorescence imaging using calcium-sensitive probe fura-2/AM. Osteoblastic proliferation was estimated by cell counting, MTS assay and ATP assay. Agonists and antagonists of calcium-sensing receptors (CaSR) as well as inhibitors of phospholipase C (PLC), SOCE and voltage-gated calcium (Cav) channels were applied to study the mechanism in detail.ResultsOur data showed that elevating [Ca2+]o evoked a sustained increase of [Ca2+]c in a dose-dependent manner. This [Ca2+]c increase was blocked by TMB-8 (Ca2+ release inhibitor), 2-APB and BTP-2 (both SOCE blockers), respectively, whereas not affected by Cav channels blockers nifedipine and verapamil. Furthermore, NPS2143 (a CaSR antagonist) or U73122 (a PLC inhibitor) strongly reduced the [Ca2+]o-induced [Ca2+]c increase. The similar responses were observed when cells were stimulated with CaSR agonist spermine. These data indicated that elevating [Ca2+]o resulted in SOCE depending on the activation of CaSR and PLC in osteoblasts. In addition, high [Ca2+]o significantly promoted osteoblastic proliferation, which was notably reversed by BAPTA-AM (an intracellular calcium chelator), 2-APB, BTP-2, TMB-8, NPS2143 and U73122, respectively, but not affected by Cav channels antagonists.ConclusionsElevating [Ca2+]o induced SOCE by triggering the activation of CaSR and PLC. This process was involved in osteoblastic proliferation induced by high level of extracellular Ca2+ concentration.

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“…Mizuno et al also showed that Ca 2+ released from CH stimulated fibronectin gene expression in dental pulp cells, a mechanism that may induce differentiation of these cells to become mineralized tissue forming cells 104 . Elevated Ca 2+ is also known to stimulate differentiation and mineralization of other dental mesenchymal cells such as cementoblasts by increasing fgf-2 expression 105 .…”

Section: Molecular Perspectives: Common Properties Of Pulp-cappimentioning

confidence: 99%

Clinical and Molecular Perspectives of Reparative Dentin Formation

Song

Kim

et al. 2017

Dental Clinics of North America

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Synopsis The long-term use of calcium hydroxide and the recent increase in the use of hydraulic calcium-silicate cements as direct pulp-capping materials provide important clues in terms of how reparative dentin may be induced to form a “biological seal” to protect the underlying pulp tissues. In this review article, we will discuss clinical and molecular perspectives of reparative dentin formation based on evidence learned from the use of these pulp-capping materials. We will also discuss the emerging role of calcium as an odontoinductive component in these pulp-capping materials.

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Elevated extracellular calcium increases fibroblast growth factor-2 gene and protein expression levels via a cAMP/PKA dependent pathway in cementoblasts

Cited by 29 publications

References 46 publications

Bioactive Polymeric Nanoparticles for Periodontal Therapy

Bioactive Polymeric Nanoparticles for Periodontal Therapy

Elevation of Extracellular Ca2+ Induces Store-Operated Calcium Entry via Calcium-Sensing Receptors: A Pathway Contributes to the Proliferation of Osteoblasts

Clinical and Molecular Perspectives of Reparative Dentin Formation

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