Elevated Carbon Monoxide to Carbon Dioxide Ratio in the Exhaled Breath of Mice Treated With a Single Dose of Lipopolysaccharide

Langeroudi, Arash Ghalyanchi; Hirsch, Charlotte M.; Estabragh, Azadeh Shojaee; Meinardi, Simone; Blake, Donald R.; Barbour, Alan G.

doi:10.1093/ofid/ofu085

Cited by 18 publications

(20 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Overall, the profile of the analyte changes in the infected immunocompetent mice corresponds to a marked systemic inflammatory response and an accompanying modulation of those pro-inflammatory cytokines and chemokines by anti-inflammatory cytokines, such as IL-10. There were several similarities to and some differences from the early responses of mice to injections of E. coli lipopolysaccharide (LPS) [ 96 ]. More specifically, 4 h after injection the LPS-treated mice showed marked elevations of both IL-6 and IL-10, and while IL-6 had declined toward normal by 24 h after injection, IL-10 remained elevated.…”

Section: Resultsmentioning

confidence: 99%

Pathogen and Host Response Dynamics in a Mouse Model of Borrelia hermsii Relapsing Fever

Crowder

Langeroudi

Estabragh

et al. 2016

Veterinary Sciences

View full text Add to dashboard Cite

Most Borrelia species that cause tick-borne relapsing fever utilize rodents as their natural reservoirs, and for decades laboratory-bred rodents have served as informative experimental models for the disease. However, while there has much progress in understanding the pathogenetic mechanisms, including antigenic variation, of the pathogen, the host side of the equation has been neglected. Using different approaches, we studied, in immunocompetent inbred mice, the dynamics of infection with and host responses to North American relapsing fever agent B. hermsii. The spirochete’s generation time in blood of infected mice was between 4–5 h and, after a delay, was matched in rate by the increase of specific agglutinating antibodies in response to the infection. After initiating serotype cells were cleared by antibodies, the surviving spirochetes were a different serotype and, as a population, grew more slowly. The retardation was attributable to the host response and not an inherently slower growth rate. The innate responses at infection peak and immediate aftermath were characterized by elevations of both pro-inflammatory and anti-inflammatory cytokines and chemokines. Immunodeficient mice had higher spirochete burdens and severe anemia, which was accounted for by aggregation of erythrocytes by spirochetes and their partially reversible sequestration in greatly enlarged spleens and elsewhere.

show abstract

Section: Resultsmentioning

confidence: 99%

Pathogen and Host Response Dynamics in a Mouse Model of Borrelia hermsii Relapsing Fever

Crowder

Langeroudi

Estabragh

et al. 2016

Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…Accurate measurements of exhaled gases requires normalization for minute volumes using the rate of CO 2 production [48]. We found that ATP injection stimulated the release of volatile organic molecules ranging from 1 to 5 carbons in length in the rst 10 minutes ( Figure 3A-H).…”

Section: Overviewmentioning

confidence: 89%

“…Breathomic analysis of natural gases and volatile organic compounds in mice was performed as described with minor modi cations [48]. Six 10-month old C57BL/6J male mice were used in this study.…”

Section: Breathomicsmentioning

confidence: 99%

Metabolic and Behavioral Features of Acute Hyperpurinergia and the Connection to Autism Spectrum Disorder

Zolkipli‐Cunningham

Naviaux

Nakayama

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background. Since 2012, studies in mice, rats, and humans have suggested that abnormalities in purinergic signaling may be a final common pathway for many genetic and environmental causes of autism spectrum disorder (ASD). The current study in mice was conducted to characterize the bioenergetic, metabolomic, breathomic, and behavioral features of acute hyperpurinergia triggered by systemic injection of the purinergic agonist and danger signal, extracellular ATP (eATP). Methods. Responses were studied in C57BL/6J mice with ASD-like behaviors in the maternal immune activation (MIA) model and controls. Basal metabolic rates, respiratory exchange ratios (RER = VCO2/VO2), and locomotor activity were measured in CLAMS cages. Plasma metabolomics measured 401 metabolites. Breathomics measured 98 volatile organic compounds. Results. A 0.5 µmol/g dose of ATP dropped whole body oxygen consumption by 74% ± 6% (mean ± SEM, 5,303 to 1,382 ml/kg/hr, p<0.0001) and rectal temperature by 6.2˚ ± 0.3˚C (p<0.0001) in 30 minutes. Over 200 metabolites from 37 different biochemical pathways where changed. MIA animals were hypersensitive to the metabolic and behavioral responses triggered by eATP and poly(IC). Breathomic and metabolomic analysis revealed changes in folate-methylation-1-carbon, purines, pyrimidines, acyl-carnitines, glycolysis, aromatic and branch-chain amino acids, Krebs cycle, glutathione, urea cycle, phospholipids, sphingolipids, eicosanoids, cholesterol, bile acids, vitamins, and microbiome metabolism similar to children with ASD. Limitations. The responses to ATP were studied in only a single genetic strain of mice (C57BL/6J). Although similar to metabolic results reported in FVB mice and a small clinical trial in children with ASD, the generalizability of these results to larger studies in humans is unknown. The chronic effects of repeated postnatal ATP exposures were not tested. Conclusions. Acute hyperpurinergia produced metabolic and behavioral changes in mice that were similar to those found in children with ASD. These behaviors and metabolic changes were associated with mitochondrial functional changes that were profound but reversible.

show abstract

“…Lipopolysaccharide (LPS)—the major component of the outer membrane of gram-negative—is released from leaky gut and represents one of the primary mechanisms for induction inflammatory response and metabolic endotoxemia. Mice and rats injected with LPS purified from E. coli are ideal models for the study of inflammation and systemic sepsis ( Bos et al., 2013 ; Langeroudi et al., 2014 ). In one study with septic mice induced by LPS, it was observed that there were increased levels of carbon monoxide (CO) and ratio of CO to CO 2 in a dose-responsive manner within hours after injection ( Langeroudi et al., 2014 ).…”

Section: Voc Signatures Emitted In Bacterial Sepsismentioning

confidence: 99%

Breath Biopsy and Discovery of Exclusive Volatile Organic Compounds for Diagnosis of Infectious Diseases

Belizário

Faintuch

Garay-Malpartida

2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Exhaled breath contains thousand metabolites and volatile organic compounds (VOCs) that originated from both respiratory tract and internal organ systems and their microbiomes. Commensal and pathogenic bacteria and virus of microbiomes are capable of producing VOCs of different chemical classes, and some of them may serve as biomarkers for installation and progression of various common human diseases. Here we describe qualitative and quantitative methods for measuring VOC fingerprints generated by cellular and microbial metabolic and pathologic pathways. We describe different chemical classes of VOCs and their role in the host cell-microbial interactions and their impact on infection disease pathology. We also update on recent progress on VOC signatures emitted by isolated bacterial species and microbiomes, and VOCs identified in exhaled breath of patients with respiratory tract and gastrointestinal diseases, and inflammatory syndromes, including the acute respiratory distress syndrome and sepsis. The VOC curated databases and instrumentations have been developed through statistically robust breathomic research in large patient populations. Scientists have now the opportunity to find potential biomarkers for both triage and diagnosis of particular human disease.

show abstract

Elevated Carbon Monoxide to Carbon Dioxide Ratio in the Exhaled Breath of Mice Treated With a Single Dose of Lipopolysaccharide

Abstract: By gas chromatographic analysis, the ratio of carbon monoxide to carbon dioxide increased in exhaled breath of 2 mouse strains injected with different doses of Escherichia coli lipopolysaccharide. The ratios correlated with inflammation biomarkers and heme oxygenase-1 expression in blood.

Cited by 18 publications

References 20 publications

Pathogen and Host Response Dynamics in a Mouse Model of Borrelia hermsii Relapsing Fever

Pathogen and Host Response Dynamics in a Mouse Model of Borrelia hermsii Relapsing Fever

Metabolic and Behavioral Features of Acute Hyperpurinergia and the Connection to Autism Spectrum Disorder

Breath Biopsy and Discovery of Exclusive Volatile Organic Compounds for Diagnosis of Infectious Diseases

Contact Info

Product

Resources

About