Electrotropism of pollen tubes of camellia and other plants

Nakamura, Norio; Fukushima, Akiyoshi; Iwayama, H.; Suzuki, H.

doi:10.1007/bf00196501

Cited by 16 publications

(21 citation statements)

References 22 publications

(18 reference statements)

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“…For instance, exposure of in vitro growing pollen tubes to macroscopic electric fields has shown that those of the species Impatiens , Camellia japonica , Erythrina , tomato, tobacco and tulip grow towards the anode 20 21 22 , whereas those of Vinca , Narcissus , Lycoris , Hedychium and Eriobotrya grow towards the cathode 21 23 24 . Other species such as Gladiolus do not exhibit any tropism 20 . Depending on the experimenter, lily pollen tubes seemed to align with the field lines of an applied electric field 25 , or not at all 20 .…”

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confidence: 99%

Influence of Electric Fields and Conductivity on Pollen Tube Growth assessed via Electrical Lab-on-Chip

Agudelo

Packirisamy

Geitmann

2016

Sci Rep

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Pollen tubes are polarly growing plant cells that are able to rapidly respond to a combination of chemical, mechanical, and electrical cues. This behavioural feature allows them to invade the flower pistil and deliver the sperm cells in highly targeted manner to receptive ovules in order to accomplish fertilization. How signals are perceived and processed in the pollen tube is still poorly understood. Evidence for electrical guidance in particular is vague and highly contradictory. To generate reproducible experimental conditions for the investigation of the effect of electric fields on pollen tube growth we developed an Electrical Lab-on-Chip (ELoC). Pollen from the species Camellia displayed differential sensitivity to electric fields depending on whether the entire cell or only its growing tip was exposed. The response to DC fields was dramatically higher than that to AC fields of the same strength. However, AC fields were found to restore and even promote pollen growth. Surprisingly, the pollen tube response correlated with the conductivity of the growth medium under different AC frequencies—consistent with the notion that the effect of the field on pollen tube growth may be mediated via its effect on the motion of ions.

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confidence: 99%

Influence of Electric Fields and Conductivity on Pollen Tube Growth assessed via Electrical Lab-on-Chip

Agudelo

Packirisamy

Geitmann

2016

Sci Rep

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“…1 It has also been shown that the electrotropic response in Camellia pollen tubes is mediated by calcium ion concentrations. 2 It has also been suggested that similar voltage readings in charophyte may be caused by the flux of acidic and basic ions, although this is controversial. 3 4 Even pure water can conduct acidic positively-charged protons by the Grotthuss mechanism.…”

Section: Discussion With Reviewersmentioning

confidence: 99%

“…Looking at actual twigs, I estimate the twig diameter to be 3/16" or .48 cm. This calculates to be a cross sectional area of 0.178 cm 2 . So the two orders of magnitude factor is reduced to 20.…”

Section: Answermentioning

confidence: 99%

Untitled

2016

WATER

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HypothesisElectric forces on and within the mature sprout system of woody plants have the strength to define the plant's growth, direction, and shape. Each branch and plant also electrically influences the shape and direction of neighboring branches and plants. AbstractThis paper has transient voltage recordings of 1) Aspen (populus grandidentata), 2) Blue Spruce, (picea pungens), 3) Sugar Maple (acer saccharum), and 4) Corn (zea maize). The recordings show that large numbers electrons are flowing upwards on and in woody plants (trees). These electrons cover the plant and are proposed to create an electron cloud within the plant's physical space. EZ water ions are proposed to collect inside the branch tips. The EZ water ions, electrons, and the earth's electric field ions are used to calculate local bud system forces. These forces as calculated are proposed to be strong enough to direct the tree branches to be three dimensional (3d) solid copies of electric field "lines of force" as it grows.A corn plant experiment shows the electron source is coming from outside the plant. An additional experiment shows a corn plant is moved by a horizontal electric field.

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“…Depending on plant species, exogenous electric fields affect the tube growth by directing the apical tube toward the cathode [Marsh and Beams, 1945;Zeijlemaker, 1956;Nakamura et al, 1991] or toward the anode [Wang et al, 1989].…”

Section: Introductionmentioning

confidence: 99%

“…Other investigations showed that the pollen tube growth direction is affected by an endogenous electric field (DC) [Weisenseel et al, 1975;Weisenseel and Jaffe, 1976;Cooke and Racusen, 1986;Obermeyer et al, 1992]. Depending on plant species, exogenous electric fields affect the tube growth by directing the apical tube toward the cathode [Marsh and Beams, 1945;Zeijlemaker, 1956;Nakamura et al, 1991] or toward the anode [Wang et al, 1989].…”

Section: Introductionmentioning

confidence: 99%

Morphological anomalies in pollen tubes of Actinidia deliciosa (kiwi) exposed to 50 Hz magnetic field

Dattilo

Bracchini

Loiselle

et al. 2005

Bioelectromagnetics

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The role of the pollen grain, with respect to the reproductive process of higher plants, is to deliver the spermatic cells to the embryo sac for egg fertilisation. Delivery occurs through the pollen tube, a self produced organ that is generated when the pollen grain reaches the stigma surface. The effect of magnetic fields on pollen tube growth was reported in a recent publication by Germanà et al. Pollen tube growth is an interesting candidate for the detailed study of the effects of electromagnetic fields on cytoplasmic structures and organelles. In this research Actinidia deliciosa (kiwifruit) pollen grains were germinated in the presence of an alternating magnetic field (50 Hz). Our results, although of preliminary nature, show that pollen tube growth is affected by magnetic fields. The analysis of the observed anomalies in the pollen tube appear to be the result of changes in the ionic charges within the pollen tube cytoplasm.

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Electrotropism of pollen tubes of camellia and other plants

Cited by 16 publications

References 22 publications

Influence of Electric Fields and Conductivity on Pollen Tube Growth assessed via Electrical Lab-on-Chip

Influence of Electric Fields and Conductivity on Pollen Tube Growth assessed via Electrical Lab-on-Chip

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Morphological anomalies in pollen tubes of Actinidia deliciosa (kiwi) exposed to 50 Hz magnetic field

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