Electrospun Silk Material Systems for Wound Healing

Wharram, Scott E.; Zhang, Xiaohui; Kaplan, David L.; McCarthy, Stephen P.

doi:10.1002/mabi.200900274

Cited by 114 publications

(52 citation statements)

References 32 publications

(109 reference statements)

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“…Silk secreted from Bombyx mori silkworms has emerged as a useful protein polymer due to its biodegradability and utility in biomaterials for regenerative medicine and drug delivery [1–4]. B. mori silk is comprised of a number of proteins: fibroin (heavy chain, light chain and P25), the key structural components, and sericin, the glue-like outer layer that coats fibroin during fiber spinning and formation of cocoons [5].…”

Section: Introductionmentioning

confidence: 99%

Impact of silk biomaterial structure on proteolysis

et al. 2015

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The goal of this study was to determine the impact of silk biomaterial structure (e.g., solution, hydrogel, film) on proteolytic susceptibility. In vitro enzymatic degradation of silk fibroin hydrogels and films was studied using a variety of proteases, including proteinase K, protease XIV, α-chymotrypsin, collagenase, matrix metalloproteinase-1 (MMP-1) and MMP-2. Hydrogels were used to assess bulk degradation while films were used to assess surface degradation. Weight loss, secondary structure determined by Fourier Transform Infrared (FT-IR) spectroscopy and degradation products analyzed via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were used to evaluate degradation through five days. Silk films were significantly degraded by proteinase K, while silk hydrogels were degraded more extensively by protease XIV and proteinase K. Collagenase preferentially degraded the β-sheet content in hydrogels while protease XIV and α-chymotrypsin degraded the amorphous structures. MMP-1 and MMP-2 degraded silk fibroin in solution resulting in a decrease in peptide fragment sizes over time. The link between primary sequence mapping with protease susceptibility provides insight into the role of secondary structure in impacting proteolytic access by comparing solution vs. solid state proteolytic susceptibility.

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Section: Introductionmentioning

confidence: 99%

Impact of silk biomaterial structure on proteolysis

et al. 2015

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“…Additionally, nanofibers have demonstrated effectiveness as wound dressings aiding in wound healing. [2][3][4] Wound dressings play a significant role in varied wounds or injuries, such as traumatic wounds, chronic wounds, and burns. Dressing materials have been shown to afford advantages in the would healing process, such as enhancing epithelialization as well as preventing wound infection.…”

Section: Introductionmentioning

confidence: 99%

Virgin olive oil blended polyurethane micro/nanofibers ornamented with copper oxide nanocrystals for biomedical applications

Amna¹,

Hassan²,

Yang³

et al. 2014

IJN

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Recently, substantial interest has been generated in using electrospun biomimetic nanofibers of hybrids, particularly organic/inorganic, to engineer different tissues. The present work, for the first time, introduced a unique natural and synthetic hybrid micronanofiber wound dressing, composed of virgin olive oil/copper oxide nanocrystals and polyurethane (PU), developed via facile electrospinning. The as-spun organic/inorganic hybrid micronanofibers were characterized by scanning electron microscopy (SEM), energy dispersive X-ray analysis, X-ray diffraction, electron probe microanalysis, and transmission electron microscopy. The interaction of cells with scaffold was studied by culturing NIH 3T3 fibroblasts on an as-spun hybrid micronanofibrous mat, and viability, proliferation, and growth were assessed. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results and SEM observation showed that the hybrid micronanofibrous scaffold was noncytotoxic to fibroblast cell culture and was found to benefit cell attachment and proliferation. Hence our results suggest the potential utilization of as-spun micronanoscaffolds for tissue engineering. Copper oxide–olive oil/PU wound dressing may exert its positive beneficial effects at every stage during wound-healing progression, and these micronanofibers may serve diverse biomedical applications, such as tissue regeneration, damaged skin treatment, wound healing applications, etc. Conclusively, the fabricated olive oil–copper oxide/PU micronanofibers combine the benefits of virgin olive oil and copper oxide, and therefore hold great promise for biomedical applications in the near future.

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“…SF has been widely used in tissue engineering because of specific functional properties, including excellent biocompatibility, good oxygen and water vapor permeability, and biodegradability [10-12]. There are large numbers of publications on SF and SF/polymer blend nanofibers [13,14], but there are few publications on the SS/SF blend nanofibers.…”

Section: Introductionmentioning

confidence: 99%

Production of silk sericin/silk fibroin blend nanofibers

et al. 2011

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Silk sericin (SS)/silk fibroin (SF) blend nanofibers have been produced by electrospinning in a binary SS/SF trifluoroacetic acid (TFA) solution system, which was prepared by mixing 20 wt.% SS TFA solution and 10 wt.% SF TFA solution to give different compositions. The diameters of the SS/SF nanofibers ranged from 33 to 837 nm, and they showed a round cross section. The surface of the SS/SF nanofibers was smooth, and the fibers possessed a bead-free structure. The average diameters of the SS/SF (75/25, 50/50, and 25/75) blend nanofibers were much thicker than that of SS and SF nanofibers. The SS/SF (100/0, 75/25, and 50/50) blend nanofibers were easily dissolved in water, while the SS/SF (25/75 and 0/100) blend nanofibers could not be completely dissolved in water. The SS/SF blend nanofibers could not be completely dissolved in methanol. The SS/SF blend nanofibers were characterized by Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry, and differential thermal analysis. FTIR showed that the SS/SF blend nanofibers possessed a random coil conformation and ß-sheet structure.

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Electrospun Silk Material Systems for Wound Healing

Cited by 114 publications

References 32 publications

Impact of silk biomaterial structure on proteolysis

Impact of silk biomaterial structure on proteolysis

Virgin olive oil blended polyurethane micro/nanofibers ornamented with copper oxide nanocrystals for biomedical applications

Production of silk sericin/silk fibroin blend nanofibers

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