Electrospun Silk Fibroin/Polycaprolactone Biomimetic Scaffold for Peripheral Nerve Regeneration

Gao, Ming; Liu, Xin; Ji, Bing; Hua, Ruheng; Li, Guicai; Zhao, Ying‐Zheng; Zhang, Luzhong; Yang, Yumin

doi:10.1166/jbt.2016.1517

Cited by 6 publications

(3 citation statements)

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“…The nutrients, metabolites, and body fluid transfer through the scaffolds are correlated with the water absorption ability . The scaffold requires a certain degree of hydrophobicity and liquid absorption capacity, which means that scaffolds could not only allow substance exchange in tissue, but also not excessively expand to oppress tissue when materials implanted in the body.…”

Section: Resultsmentioning

confidence: 99%

Tailoring degradation rates of silk fibroin scaffolds for tissue engineering

Zhang

Liu

et al. 2018

J Biomedical Materials Res

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In tissue regenerative medicine, developing tunable degradation rate of biomaterials for predictive functional outcomes remains critical. The implanted scaffolds should degrade gradually along with the tissue regeneration, and the optimal degradation rate of scaffold depends on the tissue type to be regenerated. Herein, the tunable degradation rates of silk fibroin (SF) scaffolds were fabricated through controlling dissolution, hydrolyzing conditions, and freeze-drying. The pore size, water adsorption capacity, and mechanical properties of scaffolds were associated with their average molecular weights. Moreover, in vitro cytotoxicity tests demonstrated that rapid degradation of SF scaffolds would facilitate the Schwann cells proliferation. Furthermore, in vitro enzymatic degradation and in vivo subcutaneous implantation experiments illustrated that SF scaffolds degradation behaviors could be well regulated. Immunohistochemistry staining experiments suggested that SF scaffold-degradation products could promote the endothelial cells proliferation. These results indicate that SF tunable degradation rates are promising candidates in regenerative medicine.

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Section: Resultsmentioning

confidence: 99%

Tailoring degradation rates of silk fibroin scaffolds for tissue engineering

Zhang

Liu

et al. 2018

J Biomedical Materials Res

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“…Most extracellular proteins such as collagen (which is an abundant ECM protein) have a fibrous structure with typical dimensions in the nanometer or submicrometer scales (50 to 500 nm) . Therefore, the use of electrospun nanofibrous scaffolds for tissue engineering applications has attracted considerable attention due to the straightforward processing ability of a wide range of materials such as poly(lactic acid) (PLA), poly(lactic- co -glycolic acid) (PLGA), polycaprolactone (PCL), PLGA/PCL, and synthetic polymers combined with natural collagen, gelatin, alginate, silk, chitin, and chitosan . Additionally, electrospun NFs offer a tunable porosity and a dynamically changing structure over time as the polymeric NFs degrade, allowing the seeded cells to proliferate and produce their own ECM. − …”

Section: Introductionmentioning

confidence: 99%

Plasma Functionalization of Polycaprolactone Nanofibers Changes Protein Interactions with Cells, Resulting in Increased Cell Viability

Asadian

Dhaenens

Onyshchenko

et al. 2018

ACS Appl. Mater. Interfaces

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The surface properties of electrospun scaffolds can greatly influence protein adsorption and thus strongly dictate cell-material interactions. In this study, we aim to investigate possible correlations between the surface properties of argon, nitrogen and ammonia/helium plasma-functionalized polycaprolactone (PCL) nanofibers (NFs) and their cellular interactions by examining the protein corona patterns of the plasma-treated NFs as well as the cell membrane proteins involved in cell proliferation. As a result of the performed plasma treatments, PCL NFs morphology was preserved while wettability was improved profoundly after all treatments because of the incorporation of polar surface groups. Depending on the discharge gas, different types of groups are incorporated which influenced the resultant cell-material interactions. Argon plasma-functionalized PCL NFs, only enriched by oxygen-containing functional groups, were found to show the best cell-material interactions, followed by N2 and He/NH3 plasma-treated samples. SDS-PAGE and LC-MS clearly indicated an increased protein retention compared to non-treated PCL NFs. The nine proteins best retained on plasma-treated NF are important mediators of extracellular matrix interaction, illustrating the importance thereof for cell proliferation and viability of cells. Finally, 92 proteins that can be used to differentiate the different plasma treatments are clustered and subjected to a gene ontology study, illustrating the importance of keratinization and extracellular matrix organization.

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“…This may be related to the change in microstructure of the SF and the content of the β-sheet crystal structure. 42 We thought that the β-sheet structure of SF was non-water-soluble, the structure of α helix/random coil without soaking ethanol was water-soluble. Therefore, the hydrophilicity of rigid β-sheet structure was slightly worse than that of α helix/random coil resulting in the fibers thinning.…”

Section: Resultsmentioning

confidence: 99%

Development of an electrospun polycaprolactone/silk scaffold for potential vascular tissue engineering applications

Liu

Chen

et al. 2020

Journal of Bioactive and Compatible Polymers

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Long-distance (⩾10 mm) arterial vascular defect injury was a massive challenge affecting human health. Compared with autologous transplantation, tissue-engineered scaffolds such as biocompatible silk fibroin (SF) scaffolds have been developed because they exhibit equivalent functional repair effects without adverse reactions. However, its mechanical strength and structural stability needed to be further improved to match the longer repair cycle of blood vessels while maintaining the original biological safety. Hence, we designed and prepared SF and hydrophobic polycaprolactone (PCL) composite microfibers by an improving electrospinning method. It was found that when the weight ratio of PCL to SF was 1: 1, a microfiber scaffold with high strength (6.16 N) and minimum degradability can be obtained. More importantly, compared with natural silk fibroin, the novel composite microfiber scaffolds can slightly inhibit cell infiltration and inflammation through co-culture with HUVECs in vitro and rabbit back transplantation in vivo. Furthermore, the fabricated scaffolds also demonstrated excellent structural stability in vivo because of the well-organized PCL doping in the structure. All these results indicated that the novel PCL/SF composite microfiber scaffolds were promising candidates for vascular tissue engineering applications.

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Electrospun Silk Fibroin/Polycaprolactone Biomimetic Scaffold for Peripheral Nerve Regeneration

Cited by 6 publications

References 0 publications

Tailoring degradation rates of silk fibroin scaffolds for tissue engineering

Tailoring degradation rates of silk fibroin scaffolds for tissue engineering

Plasma Functionalization of Polycaprolactone Nanofibers Changes Protein Interactions with Cells, Resulting in Increased Cell Viability

Development of an electrospun polycaprolactone/silk scaffold for potential vascular tissue engineering applications

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