Electrospun Nanostructured Scaffolds for Tissue Engineering Applications

Martins, Albino; Araújo, Jorge; Reis, Rui L.; Neves, Nuno M.

doi:10.2217/17435889.2.6.929

Cited by 182 publications

(138 citation statements)

References 151 publications

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“…Can only create fi bers. 30 nm collagen protein, [52] glactose ligand-coated poly( ε -caprolactone-co-ethyl ethane phosphate) nanofi ber [64] Cell migration, [69] tissue engineering, [119] neurite growth, [ 120] stem cell differentiation [109] Self-assembly block-copolymer Simple, fast, no special equipment needed. Higher order structures can be fabricated.…”

Section: Progress Reportmentioning

confidence: 99%

Biomimetic Nanopatterns as Enabling Tools for Analysis and Control of Live Cells

et al. 2010

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It is becoming increasingly evident that cell biology research can be considerably advanced through the use of bioengineered tools enabled by nanoscale technologies. Recent advances in nanopatterning techniques pave the way for engineering biomaterial surfaces that control cellular interactions from the nano- to the microscale, allowing more precise quantitative experimentation capturing multi-scale aspects of complex tissue physiology in vitro. The spatially and temporally controlled display of extracellular signaling cues on nanopatterned surfaces (e. g., cues in the form of chemical ligands, controlled stiffness, texture, etc.) that can now be achieved on biologically relevant length scales is particularly attractive enabling experimental platform for investigating fundamental mechanisms of adhesion-mediated cell signaling. Here, we present an overview of bio-nanopatterning methods, with the particular focus on the recent advances on the use of nanofabrication techniques as enabling tools for studying the effects of cell adhesion and signaling on cell function. We also highlight the impact of nanoscale engineering in controlling cell-material interfaces, which can have profound implications for future development of tissue engineering and regenerative medicine.

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Section: Progress Reportmentioning

confidence: 99%

Biomimetic Nanopatterns as Enabling Tools for Analysis and Control of Live Cells

et al. 2010

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“…Fibers fabricated by an electrically driven jet have been employed in various biomedical applications such as tissue engineered constructs and wound healing patches. 16 One of the advantages of ESFs is that a wide variety of macromolecules can be used for fabrication from biologically derived polymers such as gelatin, collagen, silk fibroin, chitosan, and cellulose [17][18][19][20][21] to synthetic polymers such as polyglycolide, poly (L-lactide) (PLLA), poly(e-caprolactone) (PCL), polyurethane, and poly(vinyl alcohol). 22 ESFs made of natural polymers usually possess inferior mechanical properties, while those made of synthetic polymers often lack suitable biological activities.…”

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confidence: 99%

Electrospun PLGA Fibers Incorporated with Functionalized Biomolecules for Cardiac Tissue Engineering

Lee

Lin

et al. 2014

Tissue Engineering Part A

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Structural similarity of electrospun fibers (ESFs) to the native extracellular matrix provides great potential for the application of biofunctional ESFs in tissue engineering. This study aimed to synthesize biofunctionalized poly (Llactide-co-glycolide) (PLGA) ESFs for investigating the potential for cardiac tissue engineering application. We developed a simple but novel strategy to incorporate adhesive peptides in PLGA ESFs. Two adhesive peptides derived from laminin, YIGSR, and RGD, were covalently conjugated to poly-L-lysine, and then mingled with PLGA solution for electrospinning. Peptides were uniformly distributed on the surface and in the interior of ESFs. PLGA ESFs incorporated with YIGSR or RGD or adsorbed with laminin significantly enhanced the adhesion of cardiomyocytes isolated from neonatal rats. Furthermore, the cells were found to adhere better on ESFs compared with flat substrates after 7 days of culture. Immunofluorescent staining of F-actin, vinculin, a-actinin, and Ncadherin indicated that cardiomyocytes adhered and formed striated a-actinin better on the laminin-coated ESFs and the YIGSR-incorporated ESFs compared with the RGD-incorporated ESFs. The expression of a-myosin heavy chain and b-tubulin on the YIGSR-incorporated ESFs was significantly higher compared with the expression level on PLGA and RGD-incorporated samples. Furthermore, the contraction of cardiomyocytes was faster and lasted longer on the laminin-coated ESFs and YIGSR-incorporated ESFs. The results suggest that aligned YIGSRincorporated PLGA ESFs is a better candidate for the formation of cardiac patches. This study demonstrated the potential of using peptide-incorporated ESFs as designable-scaffold platform for tissue engineering.

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“…In this technique, polymers are dissolved into a proper solvent or melted before being subjected to a very high voltage to overcome the surface tension and viscoelastic forces as well as forming different fibers (50 nm -30 µm) diameters, which feature a morphologic similarity to the extracellular matrix of natural tissue and effective mechanical properties. These nanofibrous scaffolds can be utilized to provide a better environment for cell attachment, migration, proliferation and differentiation when compared with traditional scaffolds (Martins et al, 2007). In general, the process of electrospinning is mainly affected by (i) system parameters, such as polymer molecular weight, molecular weight distribution and solution properties (e.g.…”

Section: Electrospinningmentioning

confidence: 99%