Electrospun multifunctional nanofibrous mats loaded with bioactive anemoside B4 for accelerated wound healing in diabetic mice

Abstract

With the worldwide prevalence of diabetes and considering the complicated microenvironment of diabetic wounds, the design and development of innovative multifunctional wound dressing materials are much wanted for the treatment of hard-to-heal wounds in diabetic patients. In the present study, anti-inflammatory ingredients loaded with nanofibrous wound dressing materials were manufactured by a promising blend-electrospinning strategy, and their capability for treating the diabetic wound was also systematically explored. A polymer blend consisting of Chitosan (CS) and polyvinyl alcohol (PVA) was electrospun into CS-PVA nanofibrous mats as control groups. In the meanwhile, a bioactive ingredient of Chinese medicine Pulsatilla , anemoside B4(ANE), with different contents were loaded into the electrospinning solution to construct CS-PVA-ANE nanofibrous mats. The developed CS-PVA-ANE wound dressing materials exhibited multifunctional properties including prominent water absorption, biomimetic elastic mechanical properties, and sustained ANE releasing behavior, as well as outstanding hemostatic properties. The in vitro studies showed that the CS-PVA-ANE nanofiber mats could significantly suppress lipopolysaccharide (LPS)-stimulated differentiation of pro-inflammatory (M1) macrophage subsets, and notably reduce the reactive oxygen species (ROS) generation, as well as obviously decrease inflammatory cytokine release. The in vivo animal studies showed that the CS-PVA-ANE nanofiber mats promoted the healing of diabetic wounds by significantly enhancing wound closure rates, accelerating excellent angiogenesis, promoting re-epithelization and collagen matrix deposition throughout all stages of wound healing. The present study demonstrated that CS-PVA-ANE nanofiber mats could effectively shorten the wound-healing time by inhibiting inflammatory activity, which makes them promising candidates for the treatment of hard-to-heal wounds caused by diabetes.

Electrospun Chitosan–Polyvinyl Alcohol Nanofiber Dressings Loaded with Bioactive Ursolic Acid Promoting Diabetic Wound Healing

“…An ideal wound dressing should have effective inflammatory regulation function, especially for the treatment of chronic wounds caused by diabetic ulcers, given that long-term chronic inflammation is one of the key reasons to hold back the healing process of diabetic wounds [ 29 , 41 ]. LPS-activated RAW 264.7 cells were utilized to determine the anti-inflammatory property of the CS-PVA-0.2UA nanofiber mat.…”

Polymeric Nanofibers for Drug Delivery Applications: A Recent Review

“…Zhang et al [ 53 ] used chitosan and polyvinyl alcohol electrospun nanofibers mats. Also, different levels of bioactive components from the Chinese herbal medicine dandelion were loaded into the electrospinning solution to create composite nanofiber mats to investigate the wound dressing properties of composite nanofiber materials and their ability to treat diabetic wounds.…”

Polysaccharide Electrospun Nanofibers for Wound Healing Applications

“…The most common chronic wounds are diabetic foot ulcers, bedsores, venous leg ulcers, and burns. 93 The main causes of delayed wound healing are chronic inflammation, growth factor secretion disorder, microbial infection and destruction of angiogenesis. 94 , 95 In addition, reduced proliferation, migration, and relocation of fibroblasts severely impede the wound healing process.…”

“…Chronic wounds exhibit chronic inflammation as they heal. 93 , 135 Chronic inflammation is mainly caused by high levels of reactive oxygen species (ROS), proinflammatory chemokines and bacterial infections. 136 The use of dressing materials to modulate the wound inflammatory microenvironment, including ROS removal, adsorption of inflammatory factors, and regulation of the phenotype and number of immune cells, can reduce the inflammatory response of the wound and accelerate the healing of chronic wounds.…”