Electrospun gelatin nanofibers: A facile cross-linking approach using oxidized sucrose

Jalaja, K.; James, Nirmala Rachel

doi:10.1016/j.ijbiomac.2014.11.018

Cited by 83 publications

(50 citation statements)

References 35 publications

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“…17 Electrospun nanofibers show porosity and a high surface area that facilitates the adhesion, ingrowth, and proliferation of cells. 18 Electrospun PCL/collagen nanofibers are a promising tissue-engineering substitute with great biocompatibility, low cytotoxicity, and excellent tensile strength. PCL/collagen nanofibers, on the other hand, have small pores that could inhibit cell growth into materials.…”

Section: Introductionmentioning

confidence: 99%

Electrospun polycaprolactone/collagen nanofibers cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide and genipin facilitate endothelial cell regeneration and may be a promising candidate for vascular scaffolds

Chen¹,

Zhu²,

Fu³

et al. 2019

IJN

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Purpose: A promising vascular scaffold must possess satisfying mechanical properties, great hemocompatibility, and favorable tissue regeneration. Combining natural with synthetic materials is a popular method of creating/enhancing such scaffolds. However, the effect of additional modification on the materials requires further exploration. Materials and methods: We selected polycaprolactone (PCL), which has excellent mechanical properties and biocompatibility and can be combined with collagen. Electrospun fibers created using a PCL/collagen solution were used to fashion mixed nanofibers, while separate syringes of PCL and collagen were used to create separated nanofibers, resulting in different pore sizes. Mixed and separated nanofibers were cross-linked with glutaraldehyde (GA), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and genipin; hence, we named them as mixed GA, mixed EDC (ME), mixed genipin (MG), separated GA, separated EDC (SE), and separated genipin (SG). Results: Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction showed that cross-linking did not affect the main functional groups of fibers in all groups. ME, MG, SE, and SG met the requisite mechanical properties, and they also resisted collagenase degradation. In hemocompatibility assays, only ME and MG demonstrated ideal safety. Furthermore, ME and MG presented the greatest cytocompatibility. For vascular scaffolds, rapid endothelialization helps to prevent thrombosis. According to human umbilical vein endothelial cell migration on different nanofibers, ME and MG are also successful in promoting cell migration. Conclusion: ME and MG may be promising candidates for vascular tissue engineering. The study suggests that collagen cross-linked by EDC/N-hydroxysuccinimide or genipin facilitates endothelial cell regeneration, which could be of great benefit in tissue engineering of vascular scaffolds.

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Section: Introductionmentioning

confidence: 99%

Electrospun polycaprolactone/collagen nanofibers cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide and genipin facilitate endothelial cell regeneration and may be a promising candidate for vascular scaffolds

Chen¹,

Zhu²,

Fu³

et al. 2019

IJN

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“…Oxidized sucrose has been used to cross‐link gelatin nanofibers, chitosan, and soy protein films . However, Xu et al (2015) selected 165 °C as the optimal reaction temperature to modify starch with oxidized sucrose.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Corn Starch Crosslinked with Oxidized Sucrose

et al. 2018

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In this study, oxidized sucrose is employed to crosslink corn starch at 100, 110, and 120 °C. The cross‐linked starch is analyzed by Fourier transform infrared (FTIR) and 1H nuclear magnetic resonance (1H NMR) spectroscopy. The X‐ray diffraction (XRD) analysis reveals that cross‐linked starch exhibits a “V” crystal structure after modification. Thermogravimetric (TG) analysis demonstrates that the destruction of the crystal structure increases the thermoplasticity of the starch. A decrease in the viscosity of the cross‐linked starch using a Rapid Visco Analyzer (RVA) is noted. However, compared with native starch, the swelling power of cross‐linked starch shows a negative correlation with treatment temperature. Furthermore, the cross‐linked starch that is treated at 100 °C experiences a fivefold increase in both swelling ratio and water binding capacity, compared with native starch. Additionally, low‐field nuclear magnetic resonance (low‐field NMR) analysis verifies the excellent hydrophilic properties of the cross‐linked starch. The oxidized sucrose‐modified starch (OSMS) is an alternative for improving starch‐based products, potentially in turn guiding further advancements in the pharmaceutical and food industries.

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“…The spectra of cross‐linked fibre displayed an additional shoulder peak at 1030 cm −1 band corresponding to C&bond;O&bond;C stretching of oxidised sucrose (Fig. S1) . The hypsochromic shift in amide I peak from 1642 to 1630 cm −1 and increase in its intensity could be due to Schiff's base formation between the aldehyde group of oxidised sucrose and the amino group of gelatine (Fig.…”

Section: Resultsmentioning

confidence: 98%

“…In order to increase the hydrophobicity and durability of the gelatine nanofibre mat, cross‐linking using oxidised sucrose was performed on the pure gelatine nanofibre produced (GF40). Oxidised sucrose preparation and cross‐linking procedure was performed according to the methods described by Jalaja and James . Cross‐linking was performed by immersing 0.5 g nanofibre mat in 10 mL ethanol solution containing oxidised sucrose (0.5%) for 24 h. The nanofibre mat was dried under ambient conditions in a dry chamber (relative humidity of 25%).…”

Section: Methodsmentioning

confidence: 99%

Characterisation of electrospun gelatine nanofibres encapsulated with Moringa oleifera bioactive extract

et al. 2017

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Electrospun gelatin nanofibers: A facile cross-linking approach using oxidized sucrose

Cited by 83 publications

References 35 publications

<p>Electrospun polycaprolactone/collagen nanofibers cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/<em>N-</em>hydroxysuccinimide and genipin facilitate endothelial cell regeneration and may be a promising candidate for vascular scaffolds</p>

<p>Electrospun polycaprolactone/collagen nanofibers cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/<em>N-</em>hydroxysuccinimide and genipin facilitate endothelial cell regeneration and may be a promising candidate for vascular scaffolds</p>

Synthesis and Characterization of Corn Starch Crosslinked with Oxidized Sucrose

Characterisation of electrospun gelatine nanofibres encapsulated with Moringa oleifera bioactive extract

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