Electrospun Biocomposite Nanofibrous Scaffolds for Neural Tissue Engineering

Prabhakaran, Molamma P.; Venugopal, Jayarama Reddy; Chyan, Tan Ter; Hai, Lim Beng; Chan, Casey K.; Lim, Aymeric; Ramakrishna, Seeram

doi:10.1089/ten.tea.2007.0393

Cited by 269 publications

(144 citation statements)

References 35 publications

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“…Bridging of nerve gaps after injury is a major problem in peripheral nerve regeneration. Electrospun PCL/chitosan nanofibrous scaffolds were evaluated in vitro using rat Schwann cells (RT4-D6P2T) for nerve tissue engineering [109]. The Young's modulus and strain at break of the electrospun PCL/chitosan nanofibers were better than those of the chitosan nanofibers.…”

Section: Tissue Engineeringmentioning

confidence: 99%

Preparations, properties and applications of chitosan based nanofibers fabricated by electrospinning

Sun¹,

Li²

2011

Express Polym. Lett.

215

101

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Chitosan is soluble in most acids. The protonation of the amino groups on the chitosan backbone inhibits the electrospinnability of pure chitosan. Recently, electrospinning of nanofibers based on chitosan has been widely researched and numerous nanofibers containing chitosan have been prepared by decreasing the number of the free amino groups of chitosan as the nanofibiers have enormous possibilities for better utilization in various areas. This article reviews the preparations and properties of the nanofibers which were electrospun from pure chitosan, blends of chitosan and synthetic polymers, blends of chitosan and protein, chitosan derivatives, as well as blends of chitosan and inorganic nanoparticles, respectively. The applications of the nanofibers containing chitosan such as enzyme immobilization, filtration, wound dressing, tissue engineering, drug delivery and catalysis are also summarized in detail

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Section: Tissue Engineeringmentioning

confidence: 99%

Preparations, properties and applications of chitosan based nanofibers fabricated by electrospinning

Sun¹,

Li²

2011

Express Polym. Lett.

215

101

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“…Outros estudos realizaram a incorporação de laminina e quitosana (PRABHAKARAN et al, 2008) aos polímeros PLLA e PCL respectivamente.…”

Section: Nanotecnologia E Saúdeunclassified

Nanobiotecnologia: plataforma tecnológica para biomateriais e aplicação biológica de nanoestruturas

Faria-Tischer

Tischer

2012

Biochem. Biotechnol. Rep.

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Nanobiotecnologia: plataforma tecnológica para biomateriais e aplicação biológica de nanoestruturasAbreviaturas: Nanobiotechnology, biomaterials and biological application of nanostructures Nanobiotecnologia, biomateriais e aplicação biológica de nanoestruturas ResumoA nanotecnologia é um campo da ciência que tem se desenvolvido de maneira impressionante, juntamente com a biotecnologia, misturando o conhecimento da ciência dos materiais e da química com os da biologia. Este artigo de revisão busca unir o conhecimento em biomateriais, como carboidratos e proteínas, com aqueles sobre nanopartículas, sistemas auto-organizados, nanofilmes, nanoemulsões e nanoestruturas, para encapsulação de compostos ativos. O impacto econômico dos produtos de nanotecnologia está na ordem dos trilhões de dólares. A nanobiotecnologia divide uma grande parte desse mercado. Os setores que alcançam benefícios com o desenvolvimento de produtos, técnicas, metodologias e processos em escala nanométrica são: saúde, alimentos e agroindústria. Este representa um campo fértil para aplicações em grande volume. A nanobiotecnologia é uma realidade e o conhecimento de biomateriais, das ciências biológicas e da engenharia, através da união de diferentes grupos de pesquisa em seus campos específicos, permite a concepção de produtos nunca imaginados há alguns anos. Palavras AbstRActNanotechnology is a science field that has been developping amazingly together with the biotechnology, mixing the knowledge of material science and chemistry with the biology. This review article links the know-how of biomaterials in the recent years, like carbohydrates and proteins with those about nananoparticles, self-assembled systems, nanofilms, nanoemulsions and nanoestructures for active compounds for encapsulation. The economical impact of nanotech products is in order of trillions of US dollars, and nanobiotechnology has a great share of this market. The sectors that have benefits with the development of products, technics, methodology and processes on nanometric scale are the health, food and agroindustry. The last one represents a fertile field for applications on a large scale. The nanobiotechnology is a reality and the knowledge of biomateriais, biological sciences and engineering, through the union of different research groups on their specific fields, allows the conception of products never considered many years ago.

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“…For microscale applications, microfluidics, photopatterning, etching, salt leaches, or gas foams can also be used [14][15][16][17] . Electrospinning has emerged as the most popular way to engineer fibrous substrates for tissue culture due to its flexibility and ease of production 13,[18][19][20][21][22][23] . Electrospun nanofibers are fabricated by applying a high voltage to a polymer solution causing it to repel itself and stretch across a short gap to discharge 24 .…”

Section: Introductionmentioning

confidence: 99%

Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

Whitehead

Sundararaghavan

2014

JoVE

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This procedure describes a method to fabricate a multifaceted substrate to direct nerve cell growth. This system incorporates mechanical, topographical, adhesive and chemical signals. Mechanical properties are controlled by the type of material used to fabricate the electrospun fibers. In this protocol we use 30% methacrylated Hyaluronic Acid (HA), which has a tensile modulus of ~500 Pa, to produce a soft fibrous scaffold. Electrospinning on to a rotating mandrel produces aligned fibers to create a topographical cue. Adhesion is achieved by coating the scaffold with fibronectin. The primary challenge addressed herein is providing a chemical signal throughout the depth of the scaffold for extended periods. This procedure describes fabricating poly(lactic-co-glycolic acid) (PLGA) microspheres that contain Nerve Growth Factor (NGF) and directly impregnating the scaffold with these microspheres during the electrospinning process. Due to the harsh production environment, including high sheer forces and electrical charges, protein viability is measured after production. The system provides protein release for over 60 days and has been shown to promote primary nerve cell growth. Video LinkThe video component of this article can be found at

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Electrospun Biocomposite Nanofibrous Scaffolds for Neural Tissue Engineering

Cited by 269 publications

References 35 publications

Preparations, properties and applications of chitosan based nanofibers fabricated by electrospinning

Preparations, properties and applications of chitosan based nanofibers fabricated by electrospinning

Nanobiotecnologia: plataforma tecnológica para biomateriais e aplicação biológica de nanoestruturas

Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

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