Electrospray ionization mass spectrometry, circular dichroism and SAXS studies of the (S)-hydroxynitrile lyase from Hevea brasiliensis

Uneven twins: Comparison of two enantiocomplementary hydroxynitrile lyases with α/β-hydrolase fold

“…The increase of the benzaldehyde concentration was measured continuously at 280 nm in quartz glass cuvettes following a published protocol (Hanefeld et al, 2001). In brief: citrate phosphate buffer (700 l) (100 ml contain: 24.3 ml of 0.1 M citric acid, 0.2 M K2HPO4, ad.…”

A Mild Chemo‐Enzymatic Oxidation–Hydrocyanation Protocol

“…As this acid would cause an immediate deactivation of the HbHNL it had to be removed prior to the hydrocyanation reaction. [36] By washing the reaction mixture at the end of the TEMPO/PhI(OAc) 2 oxidation of 3a with a saturated solution of NaHCO 3 , all the acetic acid was removed completely. To our satisfaction no isomerisation was detected.…”

S-selective hydroxynitrile lyase from a plant Baliospermum montanum: Molecular characterization of recombinant enzyme

“…It is a beta-rich protein and exhibited a peak at 1634/cm in amide-I region of FT-IR spectrum (data not shown). This enzyme is considered to be a homo-dimer, similar to S-HbHNL (Hanefeld et al, 2001), but different from S-MeHNL (equilibrium of homo dimers and tetramers) (Guterl et al, 2009), and S-SbHNL (hetero-tetramer, which has not an ␣/␤ hydrolase fold structure) (Lauble et al, 2002b;Schomburg and Schomburg, 2002).…”