Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

Whitehead, Tonya J.; Sundararaghavan, Harini G.

doi:10.3791/51517

Cited by 9 publications

(11 citation statements)

References 38 publications

(55 reference statements)

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“…However, a prolonged drug release is also recognized during the next 2 days, ascribable to the drug retention offered by the contribution of the hydrophobic PCL fiber network. Alternatively, Whitehead et al describe a method to fabricate a new device by integrated electrospraying/electrospinning technique to direct nerve cell growth. In this study, poly(lactic‐co‐glycolic acid) (PLGA) microspheres containing nerve growth factor (NGF) are directly impregnated into methacrylated hyaluronic acid (HA) to produce a soft fibrous scaffold able to provide a sustained protein release over 60 days.…”

Section: Towards An Integrated Approachmentioning

confidence: 99%

Additive electrospraying: a route to process electrospun scaffolds for controlled molecular release

Guarino

Altobelli

Cirillo

et al. 2015

Polymers for Advanced Techs

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Electrohydrodynamics-i.e. electrospinning, electrospraying and atomization-are gaining a growing interest in drug delivery applications, because of a large number of advantages including improved therapeutic index, localized delivery/targeting and controlled drugs toxicity level. In the past, microstructured platforms fabricated by the assembly of electrospun fibers have demonstrated to exhibit interesting features as bioactive carriers including extended surface area and high molecular permeability because of fully interconnected pore architecture, thus making the opportunity to incorporate a wide range of actives/drugs for different use. In these systems, molecular release occurs via various molecular transport pathways namely diffusion, desorption and scaffold degradation which may be tuned through a careful control of fiber morphology and composition. However, several shortcomings still concern the possibility to incorporate bioactive species, not exposing molecules to fast and/or uncontrolled denaturation, thus preserving biochemical and biological fiber functionalities. In this context, additive electro spraying (AES)-i.e. integration of electrosprayed nanoparticles into electrospun fiber network-is emerging as interesting route to control "separately" release and functional properties of the scaffolds in order to support cell activities by independent cues, during the tissue formation. Here, we describe the current advances on the use of electrospraying and/or electrospinning until more innovative integrated AES approaches to design molecularly loaded platforms able to spatially and timely release active molecules for different use in tissue engineering and molecular targeting.

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Section: Towards An Integrated Approachmentioning

confidence: 99%

Additive electrospraying: a route to process electrospun scaffolds for controlled molecular release

Guarino

Altobelli

Cirillo

et al. 2015

Polymers for Advanced Techs

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“…For example, microfluidics have been used to specifically control the regions of myotubes exposed to extracellular agrin and, hence, induce AChR cluster formation in areas with greater local concentrations of agrin (Tourovskaia, Kosar, & Folch, ; Tourovskaia, Li, & Folch, ). Thus, scaffolds may require specific regions primed with stimulatory molecules (Whitehead & Sundararaghavan, ) to enhance expression of AChR clusters in specific regions.…”

Section: Discussionmentioning

confidence: 99%

Collagen nanofibre anisotropy induces myotube differentiation and acetylcholine receptor clustering

Kung

Sillitti

Shrirao

et al. 2018

J Tissue Eng Regen Med

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To create musculoskeletal tissue scaffolds for functional integration into host tissue, myotubes must be properly aligned with native tissue and spur the formation of neuromuscular junctions. However, our understanding of myoblast differentiation in response to structural alignment is incomplete. To examine how substrate anisotropy mediates myotube differentiation, we studied C2C12 myoblasts grown on aligned collagen substrates in the presence or absence of agrin. Myoblasts grown on microfluidically patterned collagen substrates demonstrated increased multinucleated myotubes and nicotinic acetylcholine receptor (AChR) clusters. However, agrin treatment did not synergistically increase differentiation of myoblasts seeded on these patterned collagen substrates. Myoblasts grown on aligned electrospun collagen nanofibres also demonstrated increased formation of multinucleated myotubes and AChR clusters, and agrin treatment did not increase differentiation of these cells. Using fluorescently labelled collagen nanofibres, we found that AChR clustered in cells grown on nanofibres with significantly higher anisotropy and that this clustering was eliminated with agrin treatment. Interestingly, anisotropy of substrate had no effect on the localization of AChRs along the myotube, suggesting that additional signalling pathways determine the specific location of AChRs along individual myotubes. Taken together, our results suggest a novel role for fibre anisotropy in myotube differentiation, specifically AChR clustering, and that anisotropy may guide differentiation by activating similar pathways to agrin. Our data suggest that agrin treatment is not necessary for differentiation and maturation of myoblasts into myotubes when myoblasts are grown on aligned collagen substrates.

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“…In fact, a multifactorial approach has been claimed to be necessary to bridge long gaps providing a real alternative to the nerve autograft . The flexibility of the electrospinning technology used to fabricate PA guides makes it possible to not only realize topographical cues but also to improve guide design by incorporating adhesive and encapsulated chemical signals as well as living cells, opening an opportunity to take peripheral nerve regeneration a step further.…”

Section: Discussionmentioning

confidence: 99%

PEOT/PBT Guides Enhance Nerve Regeneration in Long Gap Defects

Santos

Wieringa

Moroni

et al. 2016

Adv Healthcare Materials

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Development of new nerve guides is required for replacing autologous nerve grafts for the repair of long gap defects after nerve injury. A nerve guide comprised only of electrospun fibers able to bridge a critical (15 mm) nerve gap in a rat animal model is reported for the first time. The nerve conduits are made of poly(ethylene oxide terephthalate) and poly(butylene terephthalate) (PEOT/PBT), a biocompatible copolymer composed of alternating amorphous, hydrophilic poly(ethylene oxide terephthalate), and crystalline, hydrophobic poly(butylene terephthalate) segments. These guides show suitable mechanical properties, high porosity, and fibers aligned in the longitudinal axis of the guide. In vitro studies show that both neurites and Schwann cells exhibit growth alignment with PA fibers. In vivo studies reveal that, after rat sciatic nerve transection and repair with PEOT/PBT guides, axons grow occupying a larger area compared to silicone tubes. Moreover, after repair of limiting (10 mm) and critical (15 mm) nerve gaps, PEOT/PBT guides significantly increase the percentage of regenerated nerves, the number of regenerated myelinated axons, and improve motor, sensory, and autonomic reinnervation in both gaps. This nerve conduit design combines the properties of PEOT/PBT with electrospun structure, demonstrating that nerve regeneration through long gaps can be achieved through the design of instructive biomaterial constructs.

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Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

Cited by 9 publications

References 38 publications

Additive electrospraying: a route to process electrospun scaffolds for controlled molecular release

Additive electrospraying: a route to process electrospun scaffolds for controlled molecular release

Collagen nanofibre anisotropy induces myotube differentiation and acetylcholine receptor clustering

PEOT/PBT Guides Enhance Nerve Regeneration in Long Gap Defects

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