Electrophysiological effect and the gating mechanism of astragaloside IV on l-type Ca2+ channels of guinea-pig ventricular myocytes

Zhao, Ming; Shao, Dongxue; Yu, Lifeng; Sun, Xiaohong; Wang, Yan; Hu, Huiyuan; Feng, Rui; Gao, Qinghua; Guo, Feng; Liang, Hao

doi:10.1016/j.ejphar.2015.03.082

Cited by 5 publications

(1 citation statement)

References 24 publications

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“…Studies to determine the effects of astragaloside on cardiomyocytes have primarily focused on calcium channels rather than on I f ( Zhao et al, 2015 ). The results of the present study show that astragaloside increased the I f current density in the normal and damaged SAN cells of neonatal rabbits in a voltage-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Astragaloside on Pacemaker Current and the Cytoskeleton in Rabbit Sinoatrial Node Cells Under the Ischemia and Reperfusion Condition

Liu

Peng

et al. 2018

Front. Pharmacol.

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Objective: We investigated the role of astragaloside in the treatment of sick sinus syndrome (SSS).Methods: Neonatal New Zealand rabbits were selected for the study. Rabbit sinoatrial node (SAN) cells were isolated by the method of dual enzymatic digestion and differential adherence. The injury model was prepared through simulated ischemia and reperfusion (I/R), and changes in the pacemaker current (If) were recorded using the whole-cell patch-clamp technique. The proteins F-actin and vinculin were examined between various groups of SAN cells using a microplate reader and laser scanning confocal microscopy. The mRNA level and protein expression of hyperpolarization-activated cyclic nucleotide gated potassium channel 4 (HCN4) were assessed by q-PCR and western blot method.Results: The peak current density of If was decreased to -19.64 ± 2.14 pA/pF in SAN cells after simulated I/R, and the difference was highly significant (P < 0.01). Following simulated I/R, 100, 200, or 300 μmol L-1 astragaloside was added to the extracellular solution of SAN cells; the peak current density of the If increased to -30.43 ± 1.98, -34.83 ± 1.6, and -52.72 ± 1.7 pA/pF, respectively (P < 0.01). Adding 100 μmol L-1 astragaloside to normal SAN cells also led to an enhanced peak current density of the If (P < 0.05). In a concentration-dependent manner, especially at 300 μmol/L, astragaloside was capable of increasing the expression of HCN4 and protecting the structural stability of F-actin and vinculin in the damaged SAN cells.Conclusion: We estimated that astragaloside could shorten the action potential duration 20 (APD20) and APD50 in damaged SAN cells of neonatal rabbits, thereby increasing the expression of HCN4 and the If current density in damaged SAN cells of neonatal rabbits in a voltage-dependent manner, accelerating the steady-state activation of the If channels, and protecting damaged cytoskeleton.

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Section: Discussionmentioning

confidence: 99%

The Effect of Astragaloside on Pacemaker Current and the Cytoskeleton in Rabbit Sinoatrial Node Cells Under the Ischemia and Reperfusion Condition

Liu

Peng

et al. 2018

Front. Pharmacol.

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Roles and mechanisms of natural drugs on sinus node dysfunction

Chen

2023

Biomedicine & Pharmacotherapy

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Effects of plant extracts and derivatives on cardiac K ⁺ , Nav, and Ca _v channels: a review

Seck,

Ndoye,

Kapchoup

et al. 2024

Natural Product Research

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Natural products (NPs) are endless sources of compounds for fighting against several pathologies. Many dysfunctions, including cardiovascular disorders, such as cardiac arrhythmias have their modes of action regulation of the concentration of electrolytes inside and outside the cell targeting ion channels. Here, we highlight plant extracts and secondary metabolites' effects on the treatment of related cardiac pathologies on hERG, Nav, and Cav of cardiomyocytes. The natural product's pharmacology of expressed receptors like alpha-adrenergic receptors causes Ca2+ ions influx via receptor operated Ca2+ ion channels. We also examine the NPs associated with heart contractions such as myocardial contractility by reducing the L-type calcium current and decreasing the intracellular calcium transient, inhibiting the K+ induced contractions, decreasing amplitude of myocyte shortening and showed negative ionotropic and chronotropic effects due to decreasing cytosolic Ca2+. We examine whether the NPs block potassium channels, particular the hERG channel and regulatory effects on Nav1.7.

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Electrophysiological effect and the gating mechanism of astragaloside IV on l-type Ca2+ channels of guinea-pig ventricular myocytes

Cited by 5 publications

References 24 publications

The Effect of Astragaloside on Pacemaker Current and the Cytoskeleton in Rabbit Sinoatrial Node Cells Under the Ischemia and Reperfusion Condition

The Effect of Astragaloside on Pacemaker Current and the Cytoskeleton in Rabbit Sinoatrial Node Cells Under the Ischemia and Reperfusion Condition

Roles and mechanisms of natural drugs on sinus node dysfunction

Effects of plant extracts and derivatives on cardiac K ⁺ , Nav, and Ca _v channels: a review

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Electrophysiological effect and the gating mechanism of astragaloside IV on l-type Ca2+ channels of guinea-pig ventricular myocytes

Cited by 5 publications

References 24 publications

The Effect of Astragaloside on Pacemaker Current and the Cytoskeleton in Rabbit Sinoatrial Node Cells Under the Ischemia and Reperfusion Condition

The Effect of Astragaloside on Pacemaker Current and the Cytoskeleton in Rabbit Sinoatrial Node Cells Under the Ischemia and Reperfusion Condition

Roles and mechanisms of natural drugs on sinus node dysfunction

Effects of plant extracts and derivatives on cardiac K + , Nav, and Ca v channels: a review

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Product

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Effects of plant extracts and derivatives on cardiac K ⁺ , Nav, and Ca _v channels: a review