Electrophoretic separation and immunological identification of type 2X myosin heavy chain in rat skeletal muscle

LaFramboise, William A.; Daood, Monica J.; Guthrie, Robert D.; Moretti, Paolo; Schiaffino, Stefano; Ontell, Martin P.

doi:10.1016/0304-4165(90)90181-u

Cited by 98 publications

(48 citation statements)

References 12 publications

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“…The identity of 2X and 2D MyHC was demonstrated by Western blotting (455); however, it was not established whether this MyHC is a different isoform or results from posttranslational modification of other MyHCs until definitive evidence for the presence of a distinct MyHC-2X transcript in type 2X fibers was obtained (184). Motor units composed of type 2X fibers have twitch properties (contraction and half-relaxation time) similar to those of 2A and 2B units, and their resistance to fatigue is intermediate between that of 2A and 2B units (463).…”

Section: Fiber Types In Mammalian Skeletal Musclesmentioning

confidence: 99%

Fiber Types in Mammalian Skeletal Muscles

Schiaffino

Reggiani

2011

Physiological Reviews

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Mammalian skeletal muscle comprises different fiber types, whose identity is first established during embryonic development by intrinsic myogenic control mechanisms and is later modulated by neural and hormonal factors. The relative proportion of the different fiber types varies strikingly between species, and in humans shows significant variability between individuals. Myosin heavy chain isoforms, whose complete inventory and expression pattern are now available, provide a useful marker for fiber types, both for the four major forms present in trunk and limb muscles and the minor forms present in head and neck muscles. However, muscle fiber diversity involves all functional muscle cell compartments, including membrane excitation, excitation-contraction coupling, contractile machinery, cytoskeleton scaffold, and energy supply systems. Variations within each compartment are limited by the need of matching fiber type properties between different compartments. Nerve activity is a major control mechanism of the fiber type profile, and multiple signaling pathways are implicated in activity-dependent changes of muscle fibers. The characterization of these pathways is raising increasing interest in clinical medicine, given the potentially beneficial effects of muscle fiber type switching in the prevention and treatment of metabolic diseases.

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Section: Fiber Types In Mammalian Skeletal Musclesmentioning

confidence: 99%

Fiber Types in Mammalian Skeletal Muscles

Schiaffino

Reggiani

2011

Physiological Reviews

Self Cite

2,115

2,279

View full text Add to dashboard Cite

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“…Filaments were pelleted and resuspended in 0·5 M NaCl and 10 mM NaH 2 PO 4 and subsequently diluted in SDS sample buffer and boiled for 2 min. Gel electrophoresis was performed using modifications (Talmadge & Roy 1993) of a previously described method (LaFramboise et al 1990) using 1 µg of the protein extract. SDS-PAGE comprising of a 5% separating gel and a 3% stacking gel each containing 30% glycerol was carried out for 24 h at 72 V at 4 C. The gels were either silver stained or subjected to Western blot analysis.…”

Section: Skeletal Muscle Myosin Heavy Chain Analysismentioning

confidence: 99%

Skeletal muscle glucose transporter protein responses to antenatal glucocorticoids in the ovine fetus

Gray¹,

Stonestreet²,

Thamotharan³

et al. 2006

Journal of Endocrinology

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We investigated the effects of maternal antenatal dexamethasone (Dex) treatment given as a single course (4 doses) or multiple courses (20 doses) on fetal skeletal muscle glucose transporter (GLUT) protein concentrations at 70% of gestation (106 to 107 days with term being 145 to 150 days) in the ovine fetus. Antenatal corticosteroid administration was associated with a decrease in endogenous fetal plasma cortisol concentrations (P<0·05), fetal hyperglycemia (P<0·02) and hyperinsulinemia (P<0·05). These metabolic/hormonal changes were associated with a decrease in fetal body weight (P<0·05) in the multiple course Dex group compared with the multiple course placebo group. These perturbations were associated with an increase in fetal skeletal muscle GLUT 1 concentrations that mediate basal glucose transport in the extensor digitorum lateralis and extensor digitorum longus muscles (P<0·05) 18 h after the last dose of Dex was given in the single course group.However, in the multiple course Dex group, a small increase in GLUT 1 was observed only in the biceps femoris. In contrast, both single and multiple courses of antenatal Dex were associated with an increase in the extensor digitorum lateralis and biceps femoris muscle GLUT 4 (insulin-responsive) concentrations (P<0·05). We conclude that antenatal corticosteroids perturb fetal glucose/insulin homeostasis, which is associated with increases in fetal skeletal muscle glucose transporters to compensate for and attenuate the associated catabolic fetal state. These changes consist of an increase in proteins that mediate basal glucose transport (GLUT 1) to meet immediate energy requirements of the fetal skeletal muscle with an increase in basal insulin sensitivity (GLUT 4) to compensate for the Dex-induced catabolic state after exposure to multiple courses of Dex.

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“…This process was repeated for a total of 16 different loads ranging from 90 to 1% of isometric force. The force-velocity data were fit using a modification of the equation trophoresis to evaluate ,3-MHC composition (25 Maximum developed isometric force and force/cross-sectional area. The total isometric force generated at maximum activation was analyzed from four normal individuals and seven patients with HCM (data not shown).…”

Section: Skeletal Myofiber Mechanics In Hypertrophic Cardiomyopathymentioning

confidence: 99%

Abnormal contractile properties of muscle fibers expressing beta-myosin heavy chain gene mutations in patients with hypertrophic cardiomyopathy.

et al. 1995

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Missense mutations in the 13-myosin heavy chain (, gene cause hypertrophic cardiomyopathy (HCM). As normal and mutant J-MHCs are expressed in slow-twitch skeletal muscle of HCM patients, we compared the contractile properties of single slow-twitch muscle fibers from patients with three distinct 8-MHC gene mutations and normal controls. Fibers with the 741Gly'Arg mutation (near the binding site of essential light chain) demonstrated decreased maximum velocity of shortening (39% of normal) and decreased isometric force generation (42% of normal). Fibers with the 403ArI'n mutation (at the actin interface of myosin)showed lowered force/stiffness ratio (56% of normal) and depressed velocity of shortening (50% of normal). Both the 741Gfr'Ar and 403Ar"9' mutation-containing fibers displayed abnormal force-velocity relationships and reduced power output. Fibers with the 256G1Y"lu mutation (end of ATP-binding pocket) had contractile properties that were indistinguishable from nornal. Thus there is variability in the nature and extent of functional impairments in skeletal fibers containing different fi-MHC gene mutations, which may correlate with the severity and penetrance of the disease that results from each mutation. These functional alterations likely constitute the primary stimulus for the cardiac hypertrophy that is characteristic of this disease. (J. Clin.

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Electrophoretic separation and immunological identification of type 2X myosin heavy chain in rat skeletal muscle

Cited by 98 publications

References 12 publications

Fiber Types in Mammalian Skeletal Muscles

Fiber Types in Mammalian Skeletal Muscles

Skeletal muscle glucose transporter protein responses to antenatal glucocorticoids in the ovine fetus

Abnormal contractile properties of muscle fibers expressing beta-myosin heavy chain gene mutations in patients with hypertrophic cardiomyopathy.

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