Electrophoretic methods for the analysis of nanoparticles

López‐Lorente, Ángela I.; Simonet, B.M.; Valcárcel, Miguel

doi:10.1016/j.trac.2010.10.006

Cited by 94 publications

(59 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The C-dots samples were loaded in 1.2% agarose gel and run in Tris acetate-EDTA buffer under 85 V. When the gel was visualized using a UV transilluminator, the fluorescent bands of positively charged C-dots-PEI and negatively charged C-dots-PEG migrated towards negative and positive terminals, respectively. In these cases, despite the fact that PAGE has virtue in identifying the relationship between the mobility and color of the fluorescent C-dots, it does not have high separation efficiency since the pore size of the polyacrylamide gel is typically ∼3-5 nm [182], which limits its application in separation of C-dots with a wide range of size.…”

Section: Gel Electrophoresis Ge Is a Fractionation Techniquementioning

confidence: 99%

Characterization and Analytical Separation of Fluorescent Carbon Nanodots

Gong

Liu

et al. 2017

Journal of Nanomaterials

View full text Add to dashboard Cite

Carbon nanodots (C-dots) are recently discovered fluorescent carbon nanoparticles with typical sizes of <10 nm. The C-dots have been reported to have excellent photophysical and chemical characteristics. In recent years, the advances in the development and improvement in C-dots synthesis, characterization, and applications are burgeoning. In this review, we introduce the most commonly used techniques for the characterization of C-dots. The characterization techniques for C-dots are briefly classified, described, and illustrated with applied examples. In addition, the analytical separation methods for C-dots (including electrophoresis, chromatography, density gradient centrifugation, differential centrifugation, solvent extraction, and dialysis) are included and discussed according to their analytical characteristics. The review concludes with an outlook towards the future developments in the characterization and the analytical separation of C-dots. The comprehensive overview of the characterization and the analytical separation techniques will safeguard people to use each technique more wisely.

show abstract

Section: Gel Electrophoresis Ge Is a Fractionation Techniquementioning

confidence: 99%

Characterization and Analytical Separation of Fluorescent Carbon Nanodots

Gong

Liu

et al. 2017

Journal of Nanomaterials

View full text Add to dashboard Cite

show abstract

“…Gel electrophoresis and capillary electrophoresis, which are the two electrophoretic techniques most commonly used for separation and characterization of nanoparticles [168][169][170] will be considered. Whereas most of the work with electrophoretic techniques has been devoted to the separation and characterization of nanoparticles according to size, shape and surface functionalization, using electrophoresis as diagnostic tool, the number of applications to real-world samples is still scarce.…”

Section: Electrophoresismentioning

confidence: 99%

“…In this sense, bioconjugated quantum dots [175,176] and protein-nanoparticle interactions [177] are commonly studied by CE. Although metal and metal oxide nanoparticles have been separated by using different inorganic buffers as electrolytes, the addition of ionic surfactants appears to be the most convenient mode for separation of metallic nanoparticles [168][169][170]. For instance, Liu et al [178] demonstrated that addition of sodium dodecyl sulphate to the background electrolyte improved the size separation of Au NPs, because of the charge of the NPs is then related to the number of molecules of surfactant adsorbed, which acts as a sort of in situ derivatizing agent.…”

Section: Electrophoresismentioning

confidence: 99%

Detection, characterization and quantification of inorganic engineered nanomaterials: A review of techniques and methodological approaches for the analysis of complex samples

Laborda

Bolea

Cepriá

et al. 2016

Analytica Chimica Acta

324

170

View full text Add to dashboard Cite

The increasing demand of analytical information related to inorganic engineered nanomaterials requires the adaptation of existing techniques and methods, or the development of new ones.The challenge for the analytical sciences has been to consider the nanoparticles as a new sort of analytes, involving both chemical (composition, mass and number concentration) and physical information (e.g. size, shape, aggregation). Moreover, information about the species derived from the nanoparticles themselves and their transformations must also be supplied. Whereas techniques commonly used for nanoparticle characterization, such as light scattering techniques, show serious limitations when applied to complex samples, other well-established techniques, like electron microscopy and atomic spectrometry, can provide useful information in most cases. Furthermore, separation techniques, including flow field flow fractionation, capillary electrophoresis and hydrodynamic chromatography, are moving to the nano domain, mostly hyphenated to inductively coupled plasma mass spectrometry as element specific detector.Emerging techniques based on the detection of single nanoparticles by using ICP-MS, but also coulometry, are in their way to gain a position. Chemical sensors selective to nanoparticles are in their early stages, but they are very promising considering their portability and simplicity.Although the field is in continuous evolution, at this moment it is moving from proofs-of-2 concept in simple matrices to methods dealing with matrices of higher complexity and relevant analyte concentrations. To achieve this goal, sample preparation methods are essential to manage such complex situations. Apart from size fractionation methods, matrix digestion, extraction and concentration methods capable of preserving the nature of the nanoparticles are being developed. This review presents and discusses the state-of-the-art analytical techniques and sample preparation methods suitable for dealing with complex samples. Single-and multimethod approaches applied to solve the nanometrological challenges posed by a variety of stakeholders are also presented.

show abstract

“…Electrophoretic techniques, based on the migration of charged species in a liquid under the influence of an applied electric field, have been also applied to characterize and determine NPs [54]. The coupling of capillary electrophoresis (CE) to elemental detection techniques such as ICP-MS has been already described for metal speciation purposes [55].…”

Section: Electrophoretic Techniques Coupled With Icp-msmentioning

confidence: 99%

Mass spectrometry for the characterization and quantification of engineered inorganic nanoparticles

Costa‐Fernández

Menéndez-Miranda

Bouzas-Ramos

et al. 2016

TrAC Trends in Analytical Chemistry

View full text Add to dashboard Cite

A B S T R A C TAdvancements in nanoscience enabled the synthesis of a diverse array of engineered nanomaterials. The precise control of the composition and quality of such nanomaterials is required to ensure their eventual successful application. Also importantly, the potentially adverse environmental and/or human health effects resulting from exposure to nanoparticles (NPs) is still a critical but underexplored research area. To understand and assess the potentially harmful effects of NPs a proper knowledge of their physicochemical properties is required.Among the different available analytical tools for the characterization and quantification of engineered inorganic NPs, mass spectrometry (MS) offers outstanding capabilities. The analytical capabilities of different MS-based techniques, including elemental and molecular detection, and hybrid tools derived from their coupling with different separation approaches, to identify, characterize and quantify NPs is here revised. A forward look into trends on the use of MS for more complete chemical analysis of engineered inorganic NPs is finally attempted.

show abstract

Electrophoretic methods for the analysis of nanoparticles

Cited by 94 publications

References 75 publications

Characterization and Analytical Separation of Fluorescent Carbon Nanodots

Characterization and Analytical Separation of Fluorescent Carbon Nanodots

Detection, characterization and quantification of inorganic engineered nanomaterials: A review of techniques and methodological approaches for the analysis of complex samples

Mass spectrometry for the characterization and quantification of engineered inorganic nanoparticles

Contact Info

Product

Resources

About