Electrophoretic Fractionation of Serum Glycoproteins on Cellulose Acetate

Abstract

In a procedure for the routine electrophoretic fractionation of serum glycoproteins on cellulose acetate, electrophoretic fractionation is followed by staining utilizing the periodic acid-Schiff reaction. The patterns are scanned with a recording densitometer and simultaneously quantitated with an attached integrator. For 100 healthy blood donors,the means and standard deviations were: albumin, 10.9±1.4; alpha1,18.6±1.9; alpha2, 29.6±2.3; beta, 21.6±2.0; and gamma, 19.3±1.9%, respectively.