Electron microscopy in the diagnosis of amyloidosis

Intimate structural association of amyloid and elastic fibers in systemic and cutaneous amyloidoses

“…Electron microscopically, amyloid deposition around the elastic fibere was reported in primary systemic amyloidosis (6,7), heredofamilial amyloid polyneuropathy (8), amyloid tumor of the skin (9), and secondary cutaneous amyloidosis (10). In the present study, we examined the cutaneous lesion of the 4 types of amyloidosis histopathologically using light and electron microscope and found that elastic fibers existed in the amyloid islands in all the examined forms of amyloidoses.…”

Macular amyloidosis with patchy filamentous degeneration of collagen islands

“…Macular amyloidosis occurring in the knee region has reported by Black & Wilson Jones (1970), and they described histopathologie features similar to those of the usual types of macular amyloidosis. The spatial relations between normal eollagen fibrils and amyloid filaments in eutaneous amyloidosis have been reported by many authors (Goodman et al 1972, Goerttler et al 1976, Kurban et al 1971, Shapiro et al 1970, Hashimoto & Onn 1971, Rodermund & Klingmiiller 1970, Hashimoto et al 1965, most of whom observed that col-• lagen fibrils either surrounded the amyloid islands or the amyloid islands were embedded in the collagen islands and also that amyloid and eollagen fibrils were intermingled (Goodman et al 1972, Goerttler et al 1976, Kurban et al 1971, Shapiro et al 1970, Hashimoto & Onn 1971, Rodermund & Klingmiiller 1970. However, the intimate interrelationship between the changed collagen fibrils and the amyloid filaments have been reported only by Hashimoto et al (1965), who observed the following ehanges in collagen fibrils in a case of lichen amyloidosus: 1) in some areas eollagen fibrils appeared split and their frayed ends blended into the surrounding filaments of amyloid, or from another point of view, several amyloid filaments eonverged to form a eollagen fibril; 2) in crosssections of such fraying eollagen fibrils several filamentous subunits with diameters corresponding to amyloid filaments were diseerned, but the authors did not ascertain whether the split represented the degeneration of eollagen fibrils or the formation of collagen fibrils from amyloid filaments.…”

“…The spatial relations between normal collagen fibrils and amyloid filaments in cutaneous amyloidosis have been described by many authors (Goodman et al 1972, Goerttler et al 1976, Kurban et al 1971, Shapiro et aL 1970, Hashimoto & Onn 1971, Rodermund & Klingmiiller 1970, Hashimoto et al 1965), but the intimate interrelations between the ehanged eollagen fibrils and the amyloid filaments assoeiated with eutaneous amyloidosis have been reported, to our knowledge, only by Hashimoto et al (1965).…”

Oral Triphenylmethane Food Dye Analog, Brilliant Blue G, Prevents Neuronal Loss in APPSwDI/NOS2-/- Mouse Model

“…, Fig. 6A , C-F ) [71-73]. The most notable qualitative difference in neuropathological formations observed between APP Control and APP BBG brains was the lack of degenerating cell bodies in CA1: In the pyramidal cell layer of APP Control animal hippocampus, the normal cellular arrangement was disrupted by degenerating somata and autophagosomic formations (Fig.…”