Electron detachment in H^--molecule collisions

Chromosomal Nucleoproteins:cd Studies on Reconstituted Nucleohistones From Avian Erythrocytes

“…As mentioned above, the studies on the physicochemical properties of reassociated histone-DNA complexes have been mainly concerned with the lysine-rich (f-1) fraction (2,6,8,9,26).…”

“…The lysine-rich fraction, f-1, of calf thymus histone has been suggested to be involved in the maintenance of chromosome structure (10). Probably for this reason, this fraction has been widely studied after recombination with D N A (2,6,8,9), the most informative results being given by C D studies, as the ellipticity bands of the D N A have no or very little contribution from the histone in The present study follows this line of investigation by examining the circular dichroic properties of soluble reconstituted nucleohistones from DNA and two purified histone fractions (f-2 and f-5) from avian erythrocytes.…”

“…This can be obtained either by studying native nucleoproteins after selectively removing single fractions (2, 6, 7) or by examining reasso-Abbreviations used: CD, circular dichroism; ORD, optical rotatory dispersion; DSC, 0.015 M NaCl and 0.0015 sodium citrate. ciated complexes of D N A and single histone fractions (2,6,8,9).Even if the reconstituted nucleohistones do not represent the actual state of the chromatin, they could give useful and simple model for studying the interactions in a two component system. These studies, mainly carried out by O R D and CD, have shown that the rotatory properties of D N A are altered in nucleohistone, probably indicating a change in conformation of DNA.…”

“…This can be obtained either by studying native nucleoproteins after selectively removing single fractions (2, 6, 7) or by examining reasso-Abbreviations used: CD, circular dichroism; ORD, optical rotatory dispersion; DSC, 0.015 M NaCl and 0.0015 sodium citrate. ciated complexes of D N A and single histone fractions (2,6,8,9).…”

“…The approach to the problem can be to examine spectroscopically the soluble native nucleohistone (1)(2)(3)(4)(5) in terms of structural changes of D N A and histones. Because of the heterogeneity of histone and the presence of non-histone proteins in the native complex, it would be more enlightening to separate the contributions of the single components.…”