Electrografted Diazonium Salt Layers for Antifouling on the Surface of Surface Plasmon Resonance Biosensors

Zou, Qiongjing; Kegel, Laurel L.; Booksh, Karl S.

doi:10.1021/ac504513a

Cited by 27 publications

(16 citation statements)

References 42 publications

(145 reference statements)

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“…3B). 57 Surface coverage due to fouling by crude bovine serum (undiluted, 75 mg protein per mL), measured by SPR upon 20 min exposure, was relatively good at Γ = 42 ± 4 ng cm −2 . 54,55 For the nine different peptide designs investigated, NSA was consistently lower than Γ = 80 ng cm −2 , highest resistance to serum adsorption being the feat of 3-MPA-Ser 3 -Asp 2 -OH SAMs with Γ = 23 ± 10 ng cm −2 (Fig.…”

Section: Iib 'Bioinspired' Constructs: Single Amino Acids Peptidesmentioning

confidence: 94%

“…The following year, the investigators were able to substantially decrease NSA by expanding the scope of their study to short 3-MPA-homopeptide-based SAMs. 57 In this 2015 account, anchorage to gold substrates did not rely on thiol chemistry however, 48,52 but on an electrografting reaction involving a diazonium salt of phenylalanine (Fig. 3B).…”

Section: Iib 'Bioinspired' Constructs: Single Amino Acids Peptidesmentioning

confidence: 98%

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A survey of state-of-the-art surface chemistries to minimize fouling from human and animal biofluids

2015

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Upon contact with bodily fluids, synthetic materials spontaneously acquire a layer of various species (most notably proteins) on their surface. The concern with respect to biomedical equipment, implants or devices resides in the possibility for biological processes with potentially harmful effects to ensue. In biosensor technology, the issue with this natural fouling phenomenon is that of non-specific adsorption to sensing platforms, which generates an often overwhelming interference signal that prevents the detection, not to mention the quantification, of target analytes present at considerably lower concentration. To alleviate this ubiquitous, recurrent problem - this genuine biotechnological plague - considerable research efforts have been devoted over the last few decades to engineer antifouling coatings. Extensive literature now exists that describes stealth organic adlayers capable of reducing fouling surface coverage Γ down to a few ng cm(-2)- however from biotechnologically irrelevant buffered solutions free or nearly depleted of any potentially interfering species. Regrettably indeed, few coatings are known to display/retain such level of performance when exposed to otherwise more complex, real-life biosamples (even diluted). Herein, we comprehensively review the state-of-the-art surface chemistries developed to date (January 2015) to minimize fouling from 8 such uncomparatively more challenging biological media (blood plasma, blood serum, cell lysate, cerebrospinal fluid, egg, milk, saliva, and urine) - whether of human or animal origin. Literature search for another 25 biological milieux generated no (exploitable) hit. Also discussed in this Review are the identification of the species responsible for fouling, and the dependence of antifouling properties on biosample source variability.

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Section: Iib 'Bioinspired' Constructs: Single Amino Acids Peptidesmentioning

confidence: 94%

Section: Iib 'Bioinspired' Constructs: Single Amino Acids Peptidesmentioning

confidence: 98%

A survey of state-of-the-art surface chemistries to minimize fouling from human and animal biofluids

2015

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“…Other significant examples are the construction of SPR platforms based on aryl dizonium electrographting to achieve a significant improvement in antifouling coatings [71]. Pairwise antibody footprinting is also a relevant approach for the direct immobilization of antibodies to block the unoccupied immobilized anti-Fc to prevent non-specific antibody binding [72].…”

Section: Other Strategiesmentioning

confidence: 99%

Towards the design of universal immunosurfaces for SPR-based assays: A review

Mauriz

García-Fernández

Lechuga

2016

TrAC Trends in Analytical Chemistry

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Surface biofunctionalization, including chemical activation and attachment of the bioreceptor, is an essential step to provide reliable detection of biomolecular binding events monitored by Surface Plasmon Resonance (SPR), the most employed optical biosensor, and other biosensor techniques. Recent progress in the area of immobilization procedures are aimed at producing reproducible interfacial surfaces that enable the sensitive and specific recognition of the analyte. Antibodies are still the most employed bioreceptors for SPR assays. A wide range of strategies have been proposed to maximize the SPR immunosensor performance by controlling the stability and orientation of the immobilized antibody. This article reviews the most recent advancements in random and oriented antibody immobilization approaches for SPR biosensing applications, with a special focus on the research that have been done to find universal linkers, which can allow the use of the same functionalized surface for different applications.

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“…Label-free detection platforms, which include surface plasmon resonance (SPR) (Zou et al, 2015), quartz crystal microbalance (QCM) (Tang et al, 2013), atomic force microscopy (AFM) (Zhang et al, 2014), and electrochemical impedance spectroscopy (EIS) (Date et al, 2014), show tremendous promise for quantitative protein detection. Label-free processes are usually simple, cost-effective, less time-consuming, and amenable to real-time analysis, thereby improving assay efficiency (Okuno et al, 2007; Zhao et al, 2009a; Qi and Li, 2011; Pang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Efficient label-free chemiluminescent immunosensor based on dual functional cupric oxide nanorods as peroxidase mimics

Cao

Hinman

et al. 2018

Biosensors and Bioelectronics

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Dual-functional cupric oxide nanorods (CuONRs) as peroxidase mimics are proposed for the development of a flow-through, label-free chemiluminescent (CL) immunosensor. Forming the basis of this cost-efficient, label-free immunoassay, CuONRs, synthesized using a simple hydrothermal method, were deposited onto epoxy-activated standard glass slides, followed by immobilization of biotinylated capture antibodies through a streptavidin bridge. The CuONRs possess excellent catalytic activity, along with high stability as a solid support. Antigens could then be introduced to the sensing system, forming large immunocomplexes that prevent CL substrate access to the surface, thereby reducing the CL signal in a concentration dependent fashion. Using carcinoembryonic antigen (CEA) as a model analyte, the proposed label-free immunosensor was able to rapidly determine CEA with a wide linear range of 0.1-60ngmL and a low detection limit of 0.05ngmL. This nanozyme-based immunosensor is simple, sensitive, cost-efficient, and has the potential to be a very promising platform for fast and efficient biosensing applications.

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Electrografted Diazonium Salt Layers for Antifouling on the Surface of Surface Plasmon Resonance Biosensors

Cited by 27 publications

References 42 publications

A survey of state-of-the-art surface chemistries to minimize fouling from human and animal biofluids

A survey of state-of-the-art surface chemistries to minimize fouling from human and animal biofluids

Towards the design of universal immunosurfaces for SPR-based assays: A review

Efficient label-free chemiluminescent immunosensor based on dual functional cupric oxide nanorods as peroxidase mimics

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