Electrodeposition of Three‐Dimensional Network Nanostructure PEDOT/PANI for Simultaneous Voltammetric Detection of Ascorbic Acid, Dopamine and Uric Acid

Abstract

In this study, poly(3,4-ethylenedioxythiophene) (PEDOT) and polyaniline (PANI) were electrodeposited via cyclic voltammetry (CV) on glass carbon electrode (GCE) layer by layer. The prepared electrode (GCE/PEDOT/PANI) was used for the simultaneous sensitive determination of ascorbic acid (AA), dopamine (DA) and uric acid (UA) at different oxidation potentials. The peak distances of AA and DA, DA and UA, AA and UA were 216 mV, 104 mV, 320 mV, respectively. For individual detection of AA, DA and UA, the linear ranges were 1 × 10 À 4 -1 × 10 À 2 M, 3 × 10 À 5 -1 × 10 À 3 M and 7 × 10 À 7 -1 × 10 À 4 M with detection limits of 2.42 × 10 À 5 M, 4.58 × 10 À 6 M and 2.38 × 10 À 7 M (S/N = 3), respectively. For simultaneous detection of AA, DA, and UA, the linear response ranges were 1 × 10 À 4 -1 × 10 À 3 M, 3 × 10 À 4 -1 × 10 À 2 M, and 3 × 10 À 6 -1 × 10 À 4 M with detection limits of 6.91 × 10 À 5 M, 3.66 × 10 À 5 M and 4.13 × 10 À 7 M (S/ N = 3), respectively. The GCE/PEDOT/PANI displayed good selectivity, reproducibility and storage stability, and was further applied to the detection of AA, DA, and UA in 5% normal human serum samples.

Unveiling the Fundamental Mechanisms of Graphene Oxide Selectivity on the Ascorbic Acid, Dopamine, and Uric Acid by Density Functional Theory Calculations and Charge Population Analysis

“…The minimized distortion of GO5-zigzag-B reduced the stress of the graphene plane. This behavior was confirmed by the previous DFT work by Yan et al [ 15 ]. The second most stable configuration was GO5-para, where all the pairs of carbon atoms attached to all epoxy groups were aligned in parallel, and all ten occupied carbon atoms formed a cluster.…”

“…These made graphene an ideal material for developing biosensors. Many studies have reported electrode modification to improve the simultaneous determination of AA, DA, and UA in the CV and differential pulse voltammetry (DPV) experiments [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ], e.g., the use of multilayer graphene nanoflake films (MGNFs) [ 10 ], graphitic sheets or multi-walled carbon nanotube/metal composites [ 11 , 13 , 14 , 17 , 18 , 19 , 20 , 21 ], and conducting polymers [ 12 , 15 , 16 , 22 ]. Moreover, electrical [ 23 ] and chemical [ 24 ] reduced graphene oxides (rGO) were also employed for AA/DA/UA detections due to the better conductivity than GO and their cost-effectiveness.…”

Electrochemical Dopamine Biosensor Based on Poly(3-aminobenzylamine) Layer-by-Layer Self-Assembled Multilayer Thin Film

“…The electrochemical sensor based on PPy/carbon-coated mesoporous silica (PPy/C#SiO 2 ) nanocomposite-modified glassy carbon electrodes (GCEs) demonstrated highly specific DA detection in the presence of the interference species [ 24 ]. In addition, PEDOT/PANi-modified GCEs was successfully prepared for simultaneous determination of DA, uric acid (UA) and ascorbic acid (AA) [ 25 ]. Among the common conducting polymers, PANi and its derivatives are the most popular conducting polymers in electroanalytical studies, which provide high sensitivity, selectivity, conductivity and good stability for detection of biomolecules [ 15 , 26 ].…”

Selective Voltammetric Detection of Ascorbic Acid from Rosa Canina on a Modified Graphene Oxide Paste Electrode by a Manganese(II) Complex

“…Meanwhile, for the electrochemical determination of AA, electrodes with modified surfaces have been widely used, overlapping the fulling effect due to oxidation that may occur on the conventional electrode surfaces [ 6 ]. Carbon paste (CPE) [ 12 , 19 ] and glassy carbon (GCE) [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ] modified electrodes are the most commonly used for AA analysis in the available literature. Moreover, Pt modified electrodes with 3,4-diaminobenzoic acid and neutral red [ 34 ] or screen-printed electrodes (SPE) [ 35 , 36 , 37 ].…”