Electrochromatography

Crego, Antonio L.; González, Alejandro Felipe; Marina, Marı́a Luisa

doi:10.1080/10408349608050577

Cited by 110 publications

(72 citation statements)

References 156 publications

(32 reference statements)

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“…These are determined by other physicochemical properties such as surface charge density, solvent composition, electrolyte concentration and 3. Results and discussion temperature [1]. The direction of EOF is determined by the sign of charge on the surface.…”

Section: Separation Conditionsmentioning

confidence: 99%

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Capillary electrochromatography with a silica column with a dynamically modified cationic surfactant

Zou

Liu

et al. 1999

Journal of Chromatography A

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Section: Separation Conditionsmentioning

confidence: 99%

“…to the differences in partitioning between the mobile and stationary phases, while ionic compounds are Capillary electrochromatography (CEC), which separated due to the combination of partitioning and combines the high efficiency of capillary zone electrophoresis [1]. electrophoresis (CZE) and the high selectivity of…”

Section: Introductionmentioning

confidence: 99%

Capillary electrochromatography with a silica column with a dynamically modified cationic surfactant

Zou

Liu

et al. 1999

Journal of Chromatography A

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“…CEC, which is often described as a hybrid of CZE and LC [6,7], has demonstrated higher separation efficiencies and flow permeabilities than capillary HPLC [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22]. The use of silica-based stationary phases with reversed-phase properties in CEC is well established at medium and higher mobile phase pH, where dissociated residual silanol groups provide the necessary surface charge for a suitable EOF.…”

Section: Introductionmentioning

confidence: 99%

Electrochromatographic retention of peptides on strong cation‐exchange stationary phases

Nischang¹,

Höltzel²,

Tallarek³

2010

Electrophoresis

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We analyze the systematic and substantial electrical field-dependence of electrochromatographic retention for four counterionic peptides ([Met5]enkephalin, oxytocin, [Arg8]vasopressin, and luteinizing hormone releasing hormone (LHRH) ) on a strong cation-exchange (SCX) stationary phase. Our experiments show that retention behavior in the studied system depends on the charge-selectivity of the stationary phase particles, the applied voltage, and the peptides' net charge. Retention factors of twice positively charged peptides ([Arg8]vasopressin and LHRH at pH 2.7) decrease with increasing applied voltage, whereas lower charged peptides (oxytocin and [Met5]enkephalin at pH 2.7, [Arg8]vasopressin and LHRH at pH 7.0) show a concomitant increase in their retention factors. The observed behavior is explained on the basis of electrical fieldinduced concentration polarization (CP) that develops around the SCX particles of the packing. The intraparticle concentration of charged species (buffer ions, peptides) increases with increasing applied voltage due to diffusive backflux from the enriched CP zone associated with each SCX particle. For twice charged and on the SCX phase strongly retained peptides the local increase in mobile phase ionic strength reduces the electrostatic interactions with the stationary phase, which explains the decrease of retention factors with increasing applied voltage and CP intensity. Lower charged and weaker retained peptides experience a much stronger relative intraparticle enrichment than the twice-charged peptides, which results in a net increase of retention factors with increasing applied voltage. The CP-related contribution to electrochromatographic retention of peptides on the SCX stationary phase is modulated by the applied voltage, the mobile phase ionic strength, and the peptides' net charge and could be used for selectivity tuning in difficult separations.

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“…Electroosmotic flows (EOFs) in porous media have been studied for nearly two hundred years due to their important applications in soil, petroleum and chemical engineering [1][2][3][4][5][6] since the electrokinetic effects were first observed by Reuss in 1809 in an experimental investigation on porous clay [7]. In the few past decades, there are considerable and reawakening interests in the EOF in porous media because of the conspicuous applications in biological-chemical-medical analysis [8][9][10][11] and new techniques in energy and geophysical engineering [12][13][14][15], especially in micro-and nano-scales [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Electroosmosis in homogeneously charged micro- and nanoscale random porous media

Wang

Chen

2007

Journal of Colloid and Interface Science

120

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Electroosmosis in homogeneously charged micro-and nanoscale random porous media has been numerically investigated using mesoscopic simulation methods which involve a random generation-growth method for reproducing three-dimensional random microstructures of porous media and a high-efficiency lattice Poisson-Boltzmann algorithm for solving the strongly nonlinear governing equations of electroosmosis in three-dimensional porous media. The numerical modeling and predictions of EOF in micro-and nanoscale random porous media indicate: the electroosmotic permeability increases monotonically with the porosity of porous media and the increasing rate rises with the porosity as well; the electroosmotic permeability increases with the average solid particle size for a given porosity and with the bulk ionic concentration as well; the proportionally linear relationship between the electroosmotic permeability and the zeta potential on solid surfaces breaks down for high zeta potentials. The present predictions agree well with the available experimental data while some results deviate from the predictions based on the macroscopic theories.

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Electrochromatography

Cited by 110 publications

References 156 publications

Capillary electrochromatography with a silica column with a dynamically modified cationic surfactant

Capillary electrochromatography with a silica column with a dynamically modified cationic surfactant

Electrochromatographic retention of peptides on strong cation‐exchange stationary phases

Electroosmosis in homogeneously charged micro- and nanoscale random porous media

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