Electrochemiluminescence Aptasensor for Profenofos Detection Based on Silver Nanoparticles Enhanced Luminol Luminescence System

Abstract

In this work, an electrochemiluminescence (ECL) aptasensor based on silver nanoparticles (AgNPs) for detection of profenofos was presented. The aptasensor was based on the affinity of the aptamer (Apt) with the target profenofos. When the Apt contacted with the profenofos, the ECL detection signals were weakened, indicating the successful construction of the aptasensor. In order to improve the aptasensor performance, the platinum electrode (PE) was modified by AgNPs due to their good conductivity and catalytic property. Meanwhile luminol-hydrogen peroxide was selected as a luminescence system. Through the standard curve experiment and specificity experiment, it was proved that the sensor had the advantages of low detection limit and good specificity. Under the optimum conditions, the aptasensor had a good linear response to profenofos from 0.5 μg/mL to 100 μg/mL (1.34 μM to 267.6 μM ), with a low detection limit of 0.13 ng/mL and a correlation coefficient (R 2 ) of 0.9903. The aptasensor was also successfully applied for detection of profenofos in real vegetables. Because the developed aptasensor had the following advantages of simple preparation, good stability, high selectivity and sensitivity, it would show great potential for other small molecule contamination detection.

A novel label-free electrochemiluminescence aptasensor using a tetrahedral DNA nanostructure as a scaffold for ultrasensitive detection of organophosphorus pesticides in a luminol–H2O2 system

“…The processing method of vegetables was obtained from the previous literature. 30,31 Firstly, the edible parts of those vegetables were selected and washed thoroughly. Subsequently, the vegetables were cut into 4 mm 2 or so and weighed 1 g, and were placed into centrifugal tubes of the same specification.…”

Electrochemical Aptasensor Based on Porous Reduced Graphene Oxide and Au@Fe3O4 Shell-Core Structure for Detection of Thiamethoxam in Green Leafy Vegetables

“…Aptamer had been used as a specific bio-recognition element to establish a variety of sensors. [8][9][10][11] Graphene was widely used in the modification of sensor electrodes due to its unique chemical and physical stability, excellent carrier mobility and electrical properties. [12][13][14] However, due to the strong van der Waals forces and π-π interactions from graphene basal planes, graphene tended to aggregate and destroyed its intrinsic properties.…”

Silica Template as a Morphology-Controlling Factor for Attachment of CuO Nanoparticles on 3D-Ni-Foam as a New Enzyme-Free Electrochemiluminescence Probe for Glucose Detection

“…Owing to simplicity, high sensitivity to the very low amount of analytes, high response rates, and low background signal, the ECL compensates the disadvantages of electrochemical and chemiluminescence methods. 8 The ECL sensors for a variety of analytes such as hydrogen peroxide, 9 glucose, 10,11 cysteine, 12 tetrodotoxin, 8 and pesticide [13][14][15] have been investigated in the last decade.…”