volume 32, issue 4, P1118-1126 1993
DOI: 10.1021/bi00055a018
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Daniela D. Schlereth, Werner Maentele

Abstract: IR difference spectra between the oxidized and the reduced state of horse heart cytochrome c were obtained for different temperature and pH conditions at various surface-modified electrodes using an optically transparent thin-layer electrochemical cell. These difference spectra reflect changes in protein conformation, side-chain geometries, and protonation upon the redox transition. The IR difference spectra recorded in the 10-40 degrees C temperature range showed thermally induced changes mainly in the amide-…

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