Electrochemical DNA Detection Based on the Polyhedral Boron Cluster Label

Abstract

Polyhedral boron clusters are proposed as new, chemically and biologically stable, versatile redox labels for electrochemical DNA hybridization sensors. Selective and sensitive detection of the redox labeled DNA-probe was achieved by means of covalently attached electroactive marker 7,8-dicarba-nido-undekaborate group. A nanomolar concentration of boron cluster-labeled DNA was recognized. High specificity of the analysis with the boron cluster-labeled DNA probe, including detection of single-base mismatch, was demonstrated. The above findings, together with proposed earlier use of metallacarboranes as an electrochemical label for biomolecules opens the door for a "multicolor" electrochemical coding of DNA with boron clusters and simultaneous detection of several DNA targets.

Simultaneous detection of guanine, adenine, thymine and cytosine at choline monolayer supported multiwalled carbon nanotubes film

“…Abnormal changes of DNA bases in organism suggest the deficiency of the immunity system and may indicate the presence of various diseases including cancer, epilepsy and HIV infection (Cekan and Sigurdsson, 2009;Pietrzyk et al, 2010). Therefore, the detection of DNA bases is of great significance for clinical diagnosis as well as insight into fundamental mechanisms of genetic information (Jelen et al, 2009;Abbaspour and Ghaffarinejad, 2010). In recent years, several analytical methods have been developed to detect DNA bases, such as microchip capillary electrophoresis (Wang et al, 2004), flow injection chemiluminescence (Liu and Xue, 2006), ionpairing liquid chromatography (Ganzera et al, 2006), laser-induced fluorescence detection (Wang et al, 2008), and micellar electrokinetic chromatography .…”

DNA probe modified with 3-iron bis(dicarbollide) for electrochemical determination of DNA sequence of Avian Influenza Virus H5N1

“…Working in this very vivid research field, we decided to use an alternative type of redox label, namely the chemically and biologically stable complex of carborane with Fe(III) with well-defined electrochemical properties (Corsini et al, 2006;Jelen et al, 2009;Olejniczak et al, 2009;Olejniczak 2011). Metallacarboranes are vast family of metallocenetype complexes consisting of at least one carborane cage and one or more metal cations.…”

Synthesis and in vitro antiviral activity of lipophilic pyrimidine nucleoside/carborane conjugates

“…The carboranyl cluster is a novel modifying entity for DNA/RNA-oligonucleotides that are potentially useful as therapeutic nucleic acids [20][21][22] and as molecular probes for the molecular diagnostics of infectious and genetic disease based on hybridization technology [23][24][25]. The antiviral activities of nucleoside/boron cluster conjugates has received less attention, though several 5-ortho-arbinocarboranyluracil nucleosides containing natural or modified sugar component have been studied in the past for their cytotoxicity, anticancer and antiviral activity [26].…”