Electrochemical DNA biosensor based on avidin–biotin conjugation for influenza virus (type A) detection

Chung, Da-Jung; Kim, Ki-Chul; Choi, Seong-Ho

doi:10.1016/j.apsusc.2011.06.015

Cited by 61 publications

(32 citation statements)

References 14 publications

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“…The streptavidin (or derivatives) can easily be linked on the electrode surface through physical adsorption or noncovalent bonding (Hianik et al, 2005(Hianik et al, , 2007Nimse et al, 2014;Zhou et al, 2011). Other advantages can be pointed out, namely the need of very low amounts of biotinylated aptamer (Chung et al, 2011); the fact that it is less affected by changes of buffer concentration, pH, denaturants and high temperatures; as well as the decrease of non-specific adsorption and improvement of the signal-to-noise ratio (Paniel et al, 2013;Zhou et al, 2011).…”

Section: Immobilization Of the Aptamersmentioning

confidence: 99%

“…The streptavidin and avidin proteins have four identical binding sites for biotin (Chung et al, 2011;Hayat and Marty, 2014). However, the streptavidin is preferably used over avidin, leading to fewer non-specific interactions associated to the low isoelectric point (pI) (Lim et al, 2010;Sassolas et al, 2008Sassolas et al, , 2009).…”

Section: Immobilization Of the Aptamersmentioning

confidence: 99%

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Voltammetric aptasensors for protein disease biomarkers detection: A review

Meirinho

Dias

Peres

et al. 2016

Biotechnology Advances

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An electrochemical aptasensor is a compact analytical device where the bioreceptor (aptamer) is coupled to a transducer surface to convert a biological interaction into a measurable signal (current) that can be easily processed, recorded and displayed. Since the discovery of the Systematic Evolution of Ligands by Enrichment (SELEX) methodology, the selection of aptamers and their application as bioreceptors has become a promising tool in the design of electrochemical aptasensors. Aptamers present several advantages that highlight their usefulness as bioreceptors such as chemical stability, cost effectiveness and ease of modification towards detection and immobilization at different transducer surfaces. In this review, a special emphasis is given to the potential use of electrochemical aptasensors for the detection of protein disease biomarkers using voltammetry techniques. Methods for the immobilization of aptamers onto electrode surfaces are discussed, as well as different electrochemical strategies that can be used for the design of aptasensors.

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Section: Immobilization Of the Aptamersmentioning

confidence: 99%

Section: Immobilization Of the Aptamersmentioning

confidence: 99%

Voltammetric aptasensors for protein disease biomarkers detection: A review

Meirinho

Dias

Peres

et al. 2016

Biotechnology Advances

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“…102 Another EB was fabricated by conjugation of a biotinylated probe DNA and an avidin-modified glassy carbon electrode to detect the influenza virus. 87 The current value of the biosensor was evaluated after hybridization of the probe and target DNA using CV, and hybridization was reflected by decrease in the current value. 87 Change of signal intensity after solid-surface immobilization…”

Section: Nucleic Acid Electrochemical Biosensorsmentioning

confidence: 99%

“…87 The current value of the biosensor was evaluated after hybridization of the probe and target DNA using CV, and hybridization was reflected by decrease in the current value. 87 Change of signal intensity after solid-surface immobilization…”

Section: Nucleic Acid Electrochemical Biosensorsmentioning

confidence: 99%

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Nanoscale virus biosensors: state of the art

Krejčová¹,

Michálek²,

Rodrigo³

et al. 2015

NDD

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Abstract:Since the beginning of the new millennium, viruses have shown huge epidemiological and pandemic potential: severe acute respiratory syndrome (SARS) in 2002, pandemic swine flu in 2009, and last but not least the West African Ebola outbreak in 2014. The occurrence and spread of the new virus in pandemic dimension poses a threat to the health and lives of 7 billion people worldwide. There is a growing urgency for highly sensitive and selective detection techniques, usable for a wide number of applications, including disease diagnosis, pharmaceutical research, agriculture, as well as preventive measures. Nanobiosensors represent a new promising tool for virus detection. This review gives a brief survey of the issue of viral detection, comprising diagnostics of target structure of viruses such as nucleic acids or proteins. This review covers different detection principles, methods of fabrication, and applications of virus biosensors.

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