Electrochemical detection of redox molecules secreted by Pseudomonas aeruginosa – Part 1: Electrochemical signatures of different strains

Oziat, Julie; Cohu, Thibaut; Elsen, Sylvie; Gougis, Maxime; Malliaras, George G.; Mailley, Pascal

doi:10.1016/j.bioelechem.2021.107747

Cited by 10 publications

(7 citation statements)

References 48 publications

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“…At concentrations higher than 20 μM, a larger variation in the obtained values is observed, with higher standard deviations (data not shown). This was also observed previously by Lepine et al [ 21 ] and Oziat et al [ 12 ] and is probably due to the low solubility of the PQS molecule in aqueous solution, which favors the rapid adsorption of PQS on the electrode surface.…”

Section: Resultssupporting

confidence: 85%

“…Electrochemical methods can overcome these limitations, as they are simple and sensitive approaches for detecting PQS even in complex matrices [ 1 ]. Several electrochemical sensors have been described for the detection and quantification of these molecules in biological fluids and culture media [ 11 , 12 , 13 , 14 , 15 , 16 ]. All these sensors used BDDE or GCE as the working electrode for the PQS detection, but these electrodes are not very easy to use in decentralized analyses.…”

Section: Introductionmentioning

confidence: 99%

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Highly Sensitive Detection of PQS Quorum Sensing in Pseudomonas Aeruginosa Using Screen-Printed Electrodes Modified with Nanomaterials

et al. 2022

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The rapid diagnosis of Pseudomonas aeruginosa infection is very important because this bacterium is one of the main sources of healthcare-associated infections. Pseudomonas quinolone signal (PQS) is a specific molecule for quorum sensing (QS) in P. aeruginosa, a form of cell-to-cell bacterial communication and its levels can allow the determination of the bacterial population. In this study, the development of the first electrochemical detection of PQS using screen-printed electrodes modified with carbon nanotubes (CNT-SPE) is reported. The electrochemical fingerprint of PQS was determined using different electrode materials and screen-printed electrodes modified with different nanomaterials. The optimization of the method in terms of electrolyte, pH, and electrochemical technique was achieved. The quantification of PQS was performed using one of the anodic peaks in the electrochemical fingerprint of the PQS on the CNT-SPE. The sensor exhibited a linear range from 0.1 to 15 µM, with a limit of detection of 50 nM. The sensor allowed the selective detection of PQS, with low interference from other QS molecules. The sensor was successfully applied to analysis of real samples (spiked urine and human serum samples, spiked microbiological growth media, and microbiological cultures).

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Section: Resultssupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Detection of PQS Quorum Sensing in Pseudomonas Aeruginosa Using Screen-Printed Electrodes Modified with Nanomaterials

et al. 2022

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“…Based on previously reported studies, the large separation in peak potentials and the negative potential regions of these redox peaks can be attributed to electroactive bacteria capable of using a large range of electron acceptors and substrates and able to mediate indirect EET [13,23,24]. For example, Oziat et al reported a complex electrochemical response of Pseudomonas composed of at least 7 redox peaks that are the electrochemical image of the redox secretome complexity [25]. Moreover, Xiao et al reported the CVs of Acinetobacter composed of four redox peaks spread over a potential window ranging from −0.4 to 0.9 V vs. AgCl/Ag [26].…”

Section: Influence On Electrochemical Activity Of Anodic Biofilmsmentioning

confidence: 98%

Bacterial Competition for the Anode Colonization under Different External Resistances in Microbial Fuel Cells

et al. 2022

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This study investigated the effect of external resistance (Rext) on the dynamic evolution of microbial communities in anodic biofilms of single-chamber microbial fuel cells fueled with acetate and inoculated with municipal wastewater. Anodic biofilms developed under different Rext (0, 330 and 1000 ohms, and open circuit condition) were characterized as a function of time during two weeks of growth using 16S rRNA gene sequencing, cyclic voltammetry (CV) and fluorescence microscopy. The results showed a drastic difference in power output of MFCs operated with an open circuit and those operated with Rext from 0 to 1000 ohms. Two steps during the bacterial community development of the anodic biofilms were identified. During the first four days, nonspecific electroactive bacteria (non-specific EAB), dominated by Pseudomonas, Acinetobacter, and Comamonas, grew fast whatever the value of Rext. During the second step, specific EAB, dominated by Geobacter and Desulfuromonas, took over and increased over time, except in open circuit MFCs. The relative abundance of specific EAB decreased with increasing Rext. In addition, the richness and diversity of the microbial community in the anodic biofilms decreased with decreasing Rext. These results help one to understand the bacterial competition during biofilm formation and suggest that an inhibition of the attachment of non-specific electroactive bacteria to the anode surface during the first step of biofilm formation should improve electricity production.

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“…54 With the combination of the three techniques, biofilm profiles could be added to a library to quickly identify the most common pathogens and assess treatment options. Having an "electrochemical fingerprint" 68 of various strains with a hyperspectral profile would allow for quick identification and guidance for more timeconsuming and accurate sequencing downstream methods. 54,68,69 Adapting sample volume could help bring biofilms closer to the electrode and reduce differences in compound quantifications.…”

Section: Sensors and Diagnostics Papermentioning

confidence: 99%

“…Having an "electrochemical fingerprint" 68 of various strains with a hyperspectral profile would allow for quick identification and guidance for more timeconsuming and accurate sequencing downstream methods. 54,68,69 Adapting sample volume could help bring biofilms closer to the electrode and reduce differences in compound quantifications. Earlier and later time points, as well as more frequent monitoring would also bring a heightened understanding of biofilm dynamics and formation by providing a full understanding of sample behaviour.…”

Section: Integrated P Aeruginosa Biofilm Detection In a Custom-built ...mentioning

confidence: 99%

Combining hyperspectral imaging and electrochemical sensing for detection of Pseudomonas aeruginosa through pyocyanin production

et al. 2022

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Electrochemical detection of redox molecules secreted by Pseudomonas aeruginosa – Part 1: Electrochemical signatures of different strains

Cited by 10 publications

References 48 publications

Highly Sensitive Detection of PQS Quorum Sensing in Pseudomonas Aeruginosa Using Screen-Printed Electrodes Modified with Nanomaterials

Highly Sensitive Detection of PQS Quorum Sensing in Pseudomonas Aeruginosa Using Screen-Printed Electrodes Modified with Nanomaterials

Bacterial Competition for the Anode Colonization under Different External Resistances in Microbial Fuel Cells

Combining hyperspectral imaging and electrochemical sensing for detection of Pseudomonas aeruginosa through pyocyanin production

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