Electrochemical detection of DNA immobilized on gold colloid particles modified self-assembled monolayer electrode with silver nanoparticle label

Wang, Meijia; Sun, Chunyan; Wang, Lianying; Ji, Xiaohui; Bai, Yubai; Li, Tiejin; Li, Jinghong

doi:10.1016/s0731-7085(03)00411-4

Cited by 109 publications

(53 citation statements)

References 27 publications

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“…[165][166][167] The most common electrochemical biosensors detect DNA hybridization by labeling the target DNA with a tag such as ferrocene, a redox-active enzyme, [167][168][169][170] or redox-active silver or gold nanoparticles. 171,172 Azek et al demonstrated the sensitivity of this technique with their enzyme label-based biosensor. 173 The group used a peroxidase enzyme label and a screen-printed carbon electrode to detect DNA sequences in human Cytomegalovirus.…”

Section: Future Outlook For Analyte Detection Strategiesmentioning

confidence: 99%

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

et al. 2016

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The field of centrifugal microfluidics has experienced tremendous growth during the past 15 years, especially in applications such as lab-on-a-disc (LoD) diagnostics. The strength of LoD systems lies in its potential for development into fully integrated sample-to-answer analysis systems. This review highlights the technologies necessary to develop the next generation of these systems. In addition to outlining valving and other fluid-handling operations, we discuss the recent advances and future outlook in four categories of LoD processes: reagent storage, sample preparation, nucleic acid amplification, and analyte detection strategies.

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Section: Future Outlook For Analyte Detection Strategiesmentioning

confidence: 99%

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

et al. 2016

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“…Thiol -modified oligonucleotides are commonly used for DNA-sensors and aptasensors which are based on gold electrodes [23,24]. AuNP -DNA and AgNP -DNA conjugates are used very often as labels for quantitative determination of some analytes [25,26]. However, the procedure for removal of modified electrode from the binding buffer into acidic medium for metal dissolution and analysis is very labor-consuming.…”

Section: Aptasensor Constructionmentioning

confidence: 99%

Label‐Free Electrochemical Thrombin Aptasensor Based on Ag Nanoparticles Modified Electrode

et al. 2010

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A novel label-free electrochemical method for protein detection based on redox properties of silver was developed. As recognition elements, thrombin-binding aptamers were used. Screen printed electrodes modified with silver nanoparticles (AgNP) were employed as a sensing platform for aptasensor devices. The oxidation of silver upon polarization served as a basis for analytical response. Three different thrombin binding aptamers with various surface concentrations were studied. Linear range of aptasensor response corresponded to the 10 À9 M to 10 À7 M thrombin concentration range and the detection limit was 10 À9 M.

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“…A DNA hybridization biosensor was prepared by the immobilization of target DNA onto colloid GNPs self-assembled on the cysteamine monolayer-modified gold electrode [151]. The GNPs significantly enlarged both the electrode surface and the amount of immobilized ssDNA.…”

Section: Metal Nanoparticlesmentioning

confidence: 99%