Electrochemical deoxyribonucleic acid biosensor based on carboxyl functionalized graphene oxide and poly-l-lysine modified electrode for the detection of tlh gene sequence related to vibrio parahaemolyticus

Sun, Wei; Zhang, Yuanyuan; Ju, Xiaomei; Li, Guangjiu; Gao, Hongwei; Sun, Zhenfan

doi:10.1016/j.aca.2012.09.009

Cited by 70 publications

(31 citation statements)

References 43 publications

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“…The morphological analysis revealed that the electrochemical process is more efficient compared to the chemical one, and it results in a visibly more homogeneous PLL layer. The improvement associated with the use of electrochemical method is attributable to the highly positivelycharged PLL that is deposited preferentially by electrostatic interactions [47]. Furthermore, the adsorption process and C-N bonding between PLL and BDD [48,49,59] could also play a role in the deposition of polymerized lysine.…”

Section: Resultsmentioning

confidence: 99%

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Poly-l-lysine-modified boron-doped diamond electrodes for the amperometric detection of nucleic acid bases

Niedziałkowski

Ossowski

Zięba

et al. 2015

Journal of Electroanalytical Chemistry

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Section: Resultsmentioning

confidence: 99%

“…redox behavior) of polyaniline is strongly dependent on the pH of electrolyte, and is greatly weakened at pH N 4 [46]. Su et al have fabricated the functionalized carboxyl graphene oxide (GO-COOH) at a glassy carbon electrode (GCE) and L-lysine was electropolymerized on the GO-COOH modified GCE by cyclic voltammetry (CV) [47].…”

Section: Introductionmentioning

confidence: 99%

Poly-l-lysine-modified boron-doped diamond electrodes for the amperometric detection of nucleic acid bases

Niedziałkowski

Ossowski

Zięba

et al. 2015

Journal of Electroanalytical Chemistry

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“…The positively charged -NH 2 groups of PLL could form electrostatic interactions with negatively charged carboxylic groups of GO-COOH and provide a stable lm on GCE. 27 Further, these surface morphological results were clearly stating that PLL/GO-COOH formed a thin lm on the surface of the GCE.…”

Section: Surface Characterizations Of Pll/go-cooh Modied Gce and Ft-mentioning

confidence: 90%

“…As we know that GO was decorated with many hydroxyl groups at the basal planes and carbonyl groups at the edges of the GO sheets, the enhancement of the peaks was the result of the chloroacetic acid treatment. 27 Hence these results were revealing that the large carboxyl groups appeared on the prepared GO-COOH.…”

Section: Characterization Of Synthesised Go and Go-coohmentioning

confidence: 94%

Novel poly-l-lysine/carboxyl-group enriched graphene oxide/modified electrode preparation, characterization and applications for the electrochemical determination of meloxicam in pharmaceutical tablets and blood serum

2014

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Electrochemical determination of meloxicam (MLC) was successfully determined at a poly-L-lysine (PLL)/ carboxylated graphene oxide (GO-COOH)/modified glassy carbon electrode (GCE) by cyclic voltammetry (CV) and differential pulse voltammetric (DPV) techniques. The fabricated film characterizations were investigated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and electrochemical impedance spectroscopy (EIS). The PLL/GO-COOH/GCE was employed for the electrocatalytic oxidation of MLC and the oxidation current of MLC significantly increased when compared to the bare GCE, which clearly shows that the modified electrode shows better electrocatalytic activity than the unmodified GCE. The linear range of the determination of MLC has to be 4 to 104 mM. The low limit of detection (LOD) and sensitivity was found as 0.87 mM and 0.228 mA mM À1 cm À2 . The analytical performance of this proposed sensor has been evaluated in commercially available pharmaceutical tablets and the practicality of the proposed sensor further demonstrated in a real blood serum sample. The obtained results for commercially available tablets were almost equal to the labelled claim of the tablets; from these results it was clearly evident that the proposed electrode shows good catalytic activity towards MLC.

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“…Fernandes et al reported the construction of electrochemical genosensor based on multiwalled carbon nanotube-chitosan-bismuth and lead sulfide nanoparticle for the detection of pathogenic Aeromonas [31]. Our group applied an electrochemical DNA sensor based on carboxyl-functionalized graphene oxide and poly-L-lysine-modified electrode for the detection of tlh gene sequences that related to Vibrio parahaemolyticus [32]. Also, a dendritic nanogold and electrochemically reduced graphene-modified electrode were prepared for the detection of specific DNA sequence of Listeria monocytogenes [33].…”

Section: Introductionmentioning

confidence: 98%

Electrochemical DNA sensor for Staphylococcus aureus nuc gene sequence with zirconia and graphene modified electrode

Sun

Wang

et al. 2015

J Solid State Electrochem

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In this paper, zirconia (ZrO 2 ) and graphene (GR) nanocomposite was electrodeposited on the surface of carbon ionic liquid electrode (CILE), which was used to construct an electrochemical DNA biosensor. GR was electroreduced from graphene oxide by potentiostatic method, and ZrO 2 nanoparticle was further electrodeposited on GR/CILE by cycling voltammetric scan in a ZrOCl 2 solution. The presence of GR on the electrode surface can provide a highly conductive interface with large surface area for the loading of ZrO 2 nanoparticles. Single-stranded DNA (ssDNA) probe sequences with phosphate group at the 5′ end could be easily immobilized on the surface of ZrO 2 /GR/CILE due to the strong affinity between ZrO 2 and phosphate groups. The ssDNA/ZrO 2 /GR/CILE was applied to hybridize with the target ssDNA sequence, and methylene blue (MB) was used as the electrochemical indicator. Due to the different binding models of MB with double-stranded DNA and ssDNA on the electrode surface, electrochemical response of MB was decreased after the hybridization reaction. Under the optimal conditions, the reduction peak current of MB was proportional to the concentration of Staphylococcus aureus nuc gene sequence in the range from 1.0×10 −13 to 1.0×10 −6 mol L −1 with the detection limit of 3.23 × 10 −14 mol L −1 (3σ). The electrochemical DNA sensor exhibited good selectivity to various mismatched ssDNA sequences, and the polymerase chain reaction amplification products of S. aureus nuc gene sequence were further detected with satisfactory results. Therefore, this electrochemical DNA sensor with ZrO 2 nanoparticles and GR nanosheet modified electrode could be used for the detection of specific ssDNA sequence in real biological samples.

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Electrochemical deoxyribonucleic acid biosensor based on carboxyl functionalized graphene oxide and poly-l-lysine modified electrode for the detection of tlh gene sequence related to vibrio parahaemolyticus

Cited by 70 publications

References 43 publications

Poly-l-lysine-modified boron-doped diamond electrodes for the amperometric detection of nucleic acid bases

Poly-l-lysine-modified boron-doped diamond electrodes for the amperometric detection of nucleic acid bases

Novel poly-l-lysine/carboxyl-group enriched graphene oxide/modified electrode preparation, characterization and applications for the electrochemical determination of meloxicam in pharmaceutical tablets and blood serum

Electrochemical DNA sensor for Staphylococcus aureus nuc gene sequence with zirconia and graphene modified electrode

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