Electrochemical Biosensors: Recommended Definitions and Classification

Venot, Daniel R. Thé; Tóth, Klára; Durst, Richard A.; Wilson, George S.

doi:10.1515/iupac.71.0635

Cited by 197 publications

(278 citation statements)

References 2 publications

(3 reference statements)

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“…Detection of numerous signaling molecules has advanced greatly with the development of biosensors, which transform biochemical information into analytically useful signals (Thévenot et al, 2001). Fluorescent biosensors, for example, allow imaging and quantification in living cells with high spatial and temporal resolution, and are used to detect the activity of signaling intermediates such as cAMP, glutamate, tryptophan, and Ca 2+ (VanEngelenburg and Palmer, 2008).…”

Section: Introductionmentioning

confidence: 99%

Single-Cell Resolution and Quantitation of Targeted Glucocorticoid Delivery in the Thymus

et al. 2019

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SUMMARY Glucocorticoids are lipid-soluble hormones that signal via the glucocorticoid receptor (GR), a ligand-dependent transcription factor. Circulating glucocorticoids derive from the adrenals, but it is now apparent that paracrine glucocorticoid signaling occurs in multiple tissues. Effective local glucocorticoid concentrations and whether glucocorticoid delivery can be targeted to specific cell subsets are unknown. We use fluorescence detection of chromatin-associated GRs as biosensors of ligand binding and observe signals corresponding to steroid concentrations over physiological ranges in vitro and in vivo. In the thymus, where thymic epithelial cell (TEC)-synthesized glucocorticoids antagonize negative selection, we find that CD4+CD8+TCRhi cells, a small subset responding to self-antigens and undergoing selection, are specific targets of TEC-derived glucocorticoids and are exposed to 3-fold higher levels than other cells. These results demonstrate and quantitate targeted delivery of paracrine glucocorticoids. This approach may be used to assess in situ nuclear receptor signaling in a variety of physiological and pathological contexts.

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Section: Introductionmentioning

confidence: 99%

Single-Cell Resolution and Quantitation of Targeted Glucocorticoid Delivery in the Thymus

et al. 2019

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“…ferrocene derivatives, K 4 [Fe(CN) 6 ], ruthenium or Os-complexes) are added to the sample and used as freely-diffusing ET shuttles. Some other materials are also used, e.g.…”

Section: Second Generation Of Amperometric Biosensorsmentioning

confidence: 99%

“…Typically, the reaction under investigation would either generate a measurable current (amperometric), a measurable potential or charge accumulation (potentiometric) or measurably alter conductive properties of a medium between electrodes (conductometric) [4]. References are also made to other types of electrochemical detection techniques, such as impedimetric, which measures impedance (both resistance and reactance) [5], and field-effect, which uses transistor technology to measure current as a result of a potentiometric effect at a gate electrode [6].…”

Section: Introductionmentioning

confidence: 99%

“…On the basis of the transducing element, biosensors can be categorized as electrochemical, optical, piezoelectric, and thermal sensors. Depending upon the nature of electrochemical changes in detection during a biorecognition event, electrochemical biosensors fall into one of four categories: amperometric, potentiometric, impedimetric and conductometric; field effect transistors may be also used as transduction devices [6].…”

Section: Introductionmentioning

confidence: 99%

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Chemically Modified Electrodes in Biosensing

Ciucu¹

2014

J Biosens Bioelectron

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The development of chemical sensors has received a great deal of scientific interest in the last decades. Not only the chemical industry may benefit from these sensors but also the food industry, bio-industry, medicine, environmental control because of their capability to give continuously and reversibly a selective and fast response to the presence of a specific compound in a complex mixture of components, without perturbing the system. Biosensors combine the power of analytical detection techniques with the specificity of biological recognition system and therefore they are the most promising devices today about this selectivity. Furthermore, biosensors possess many unique features such as compact size, simplicity of use, one-step reagentless analysis, absence of radioactivity, etc., that make them very attractive alternatives to conventional bioanalytical techniques. The present short review highlights some modern aspects of Chemically Modified Electrodes (CMEs) based on redox enzymes used in amperometric biosensing, a detection method which has already found a large number of applications in health care, food industry and environmental analysis. Some relevant applications of amperometric biosensors based on CMEs to real sample analysis are also presented and some possible future trends highlighted.

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“…According to the most recent definition released by IUPAC and reported by Thévenot et al (2001) [1], a biosensor (electrochemical) is classified as a "selfcontained integrated device, which is capable of providing specific quantitative or semi-quantitative analytical information using a biological recognition element (biochemical receptor) which is retained in direct spatial contact with an electrochemical transduction element." As stated, this definition includes those devices that are both disposable after one measurement and unable to control the analyte concentration continuously or after rapid and reproducible regeneration, i.e.…”

Section: Introductionmentioning

confidence: 99%

Electrospinning for High Performance Sensors

Macagnano¹,

Zampetti²,

Kny³

2015

NanoScience and Technology

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The series NanoScience and Technology is focused on the fascinating nano-world, mesoscopic physics, analysis with atomic resolution, nano and quantum-effect devices, nanomechanics and atomic-scale processes. All the basic aspects and technology-oriented developments in this emerging discipline are covered by comprehensive and timely books. The series constitutes a survey of the relevant special topics, which are presented by leading experts in the field. These books will appeal to researchers, engineers, and advanced students.More information about this series at

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Electrochemical Biosensors: Recommended Definitions and Classification

Cited by 197 publications

References 2 publications

Single-Cell Resolution and Quantitation of Targeted Glucocorticoid Delivery in the Thymus

Single-Cell Resolution and Quantitation of Targeted Glucocorticoid Delivery in the Thymus

Chemically Modified Electrodes in Biosensing

Electrospinning for High Performance Sensors

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