Electrochemical aptasensor for detecting tetracycline in milk

Abstract

A rapid, simple and sensitive biosensor system for tetracycline detection is very important in food safety. In this paper we developed a label-free aptasensor for electrochemical detection of tetracycline. According to the electrochemical impendence spectroscopy (EIS) analysis, there was a linear relationship between the concentration of tetracycline and the electron transfer resistance from 10 to 3000 ng ml−1 of the tetracycline concentration. The detection limit was 10 ng ml−1 in 15 min detection duration. The prepared aptasensor showed a good reproducibility with an acceptable stability in tetracycline detection. The recoveries of tetracycline in spiked milk samples were in the range of 88.1%–94.2%. The aptasensor has sensitivity 98% and specificity of 100%.

Tetracycline Antibiotics: Elucidating the Electrochemical Fingerprint and Oxidation Pathway

“…TCs are especially effective against a broad variety of Gram-positive and Gram-negative bacteria, such as Staphylococcus, Streptococcus, Pneumococcus, Gonococcus, Cholera, Dysentery bacillis, Pertussis, Rickettsia, Chlamydia, and Mycoplasma [1,2]. Since their first introduction into medicine in the 1940s, antibiotics have been widely used in livestock farming-as veterinary medicines, feed additives, growth promoters [3], or to prevent/treat mastitis and metritis in cows [4]-and in healthcare for the treatment of many different infections, such as respiratory tract infections, urethritis and severe acne, or even malaria [5,6]. TCs can be divided into four main types: tetracycline (TET), doxycycline (DOXY), oxytetracycline (OXY), and chlortetracycline (CHL), which are most often applied to livestock animals (including honeybees) because of their affordability [5].…”

“…Biorecognition is one of the most common strategies and uses aptamer-based sensors [23][24][25][26][27][28][29][30][31][32], which are the most recent and commonly found in the literature, followed by enzyme-linked immunoassay (ELISA) functionalization [14,15,33,34], and sensors based on molecular imprinted polymers (MIPs) [35,36] or antibodies [8,37,38] (see Table S2). Furthermore, the oxidation of TCs has been reported using diverse types of electrodes and materials, ranging from metallic nanoparticles [39][40][41][42][43][44][45], graphene oxide [46][47][48][49][50], composites [43,[51][52][53][54][55], gold [3,11,56], boron-doped diamond electrode (BDDE) [57][58][59][60], ruthenium oxide-hexacyanor-uthenate (RuO-RuCN) [61], or multiwall carbon nanotubes (MWN...…”

Aptamer-Based Biosensors for Antibiotic Detection: A Review

“…Since tetracycline detection is very important in food safety, plenty of research has been devoted to the development of sensitive, selective, and specific aptasensors on the basis of different sensing methods, like colorimetric [ 195 , 211 , 212 , 213 , 214 , 215 ], surface-enhanced Raman spectroscopic [ 216 , 217 ], FAM-labelled and label-free [ 218 , 219 , 220 , 221 ] fluorometric, photoelectrochemical [ 208 , 222 , 223 ], electrochemiluminescent [ 93 ], impedimetric and amperometric electrochemical [ 41 , 224 , 225 , 226 , 227 , 228 , 229 , 230 , 231 , 232 , 233 , 234 , 235 , 236 , 237 , 238 , 239 ] methods. Among them, the proposed electrochemical aptasensors by Jahanbani’s team [ 236 ] showed ultrahigh sensitivity towards tetracycline.…”

Aptamer-based Biosensors for Antibiotic Detection: A Review

“…Le et al [225] presented an impedimetric electrochemical aptasensor for the detection of tetracycline. Tetracycline specific amino-modified aptamers were immobilized onto the surface of the Au electrode and subsequently tetracycline was added.…”