Electrochemical and biosensing properties of an FAD-dependent glucose dehydrogenase from Trichoderma virens

Wijayanti, Sudarma Dita; Schachinger, Franziska; Ludwig, Roland; Haltrich, Dietmar

doi:10.1016/j.bioelechem.2023.108480

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…100,101 Many redox polymers, used as electron transfer mediators and enzyme immobilizing matrices, offered improved bioelectrode performance. 100,102 Conducting polymers have often been used for improving signal transduction and amplifications and to serve as an immobilization matrix. 101,103,104 Polyelectrolytes, which have ionic properties, help to incorporate various species with sensing/mediating/transducing properties and serve as a matrix for immobilizing the biorecognition elements.…”

Section: Materials For Bioelectrode Fabricationmentioning

confidence: 99%

“…The main purposes of using these materials are to improve the electron/charge transfer efficiency, increase the active surface area of the electrode, and serve as an immobilization matrix to provide better stability and reusability of the biorecognition element. Various bio/polymers such as, polysaccharides, proteins, and their composites are widely used as the immobilization matrix. − Synthetic polymers such as redox polymers and conducting polymers have also been explored for this purpose. , Many redox polymers, used as electron transfer mediators and enzyme immobilizing matrices, offered improved bioelectrode performance. , Conducting polymers have often been used for improving signal transduction and amplifications and to serve as an immobilization matrix. ,, Polyelectrolytes, which have ionic properties, help to incorporate various species with sensing/mediating/transducing properties and serve as a matrix for immobilizing the biorecognition elements. − The role of polyelectrolytes in the development of the sensing systems and the architectures of the sensing layers have been reviewed . Organic frameworks, such as metal–organic frameworks (MOFs) and covalent organic frameworks (COFs), have also been explored for biosensor applications.…”

Section: Materials For Bioelectrode Fabricationmentioning

confidence: 99%

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Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Kalita,

Gogoi,

Minteer

et al. 2023

ACS Meas. Sci. Au

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The critical performance factors such as selectivity, sensitivity, operational and storage stability, and response time of electrochemical biosensors are governed mainly by the function of their key component, the bioelectrode. Suitable design and fabrication strategies of the bioelectrode interface are essential for realizing the requisite performance of the biosensors for their practical utility. A multifaceted attempt to achieve this goal is visible from the vast literature exploring effective strategies for preparing, immobilizing, and stabilizing biorecognition elements on the electrode surface and efficient transduction of biochemical signals into electrical ones (i.e., current, voltage, and impedance) through the bioelectrode interface with the aid of advanced materials and techniques. The commercial success of biosensors in modern society is also increasingly influenced by their size (and hence portability), multiplexing capability, and coupling in the interface of the wireless communication technology, which facilitates quick data transfer and linked decision-making processes in real-time in different areas such as healthcare, agriculture, food, and environmental applications. Therefore, fabrication of the bioelectrode involves careful selection and control of several parameters, including biorecognition elements, electrode materials, shape and size of the electrode, detection principles, and various fabrication strategies, including microscale and printing technologies. This review discusses recent trends in bioelectrode designs and fabrications for developing electrochemical biosensors. The discussions have been delineated into the types of biorecognition elements and their immobilization strategies, signal transduction approaches, commonly used advanced materials for electrode fabrication and techniques for fabricating the bioelectrodes, and device integration with modern electronic communication technology for developing electrochemical biosensors of commercial interest.

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Section: Materials For Bioelectrode Fabricationmentioning

confidence: 99%

Section: Materials For Bioelectrode Fabricationmentioning

confidence: 99%

Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Kalita,

Gogoi,

Minteer

et al. 2023

ACS Meas. Sci. Au

View full text Add to dashboard Cite

show abstract

“…Typically, two enzymes, α-1,4-glucosidase and GOx, are used to this end [ 46 , 47 , 48 , 49 ]. A recent report on a maltose biosensor developed by our group shows the possibility of a single enzyme capable of maltose detection [ 50 ]. GDH from Trichoderma virens from the until then unexplored GDH class II was used as the biorecognition element and wired by an osmium redox polymer to a graphite electrode.…”

Section: Enzyme-based Biosensors For Food Analysismentioning

confidence: 99%

Recent Advances in Electrochemical Enzyme-Based Biosensors for Food and Beverage Analysis

Wijayanti,

Tsvik,

Haltrich

2023

Foods

Self Cite

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Food analysis and control are crucial aspects in food research and production in order to ensure quality and safety of food products. Electrochemical biosensors based on enzymes as the bioreceptors are emerging as promising tools for food analysis because of their high selectivity and sensitivity, short analysis time, and high-cost effectiveness in comparison to conventional methods. This review provides the readers with an overview of various electrochemical enzyme-based biosensors in food analysis, focusing on enzymes used for different applications in the analysis of sugars, alcohols, amino acids and amines, and organic acids, as well as mycotoxins and chemical contaminants. In addition, strategies to improve the performance of enzyme-based biosensors that have been reported over the last five years will be discussed. The challenges and future outlooks for the food sector are also presented.

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“…Additionally, according to our previous studies, polyethylene glycol diglyceryl ether (PEGDGE) was a good polymer to immobilize the enzyme on the bioelectrode and without affecting the enzyme activity [23]. Since polyvinyl acetate (PVA) gel has good mechanical strength and stability, which enables PVA gel electrolytes to maintain stable performance in various applications, PVA gel electrolytes were used in this experiment [24,25] .…”

Section: Introductionmentioning

confidence: 99%

Wearable cellulose textile matrix self-powered biosensor sensing lactate in human sweat

夏,

Zuo,

et al. 2023

Preprint

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A lactate self-powered biosensor was assembled for the detection of lactate in sweat based on flexible textile matrix enzymatic electrodes. A porous three-dimensional electrode with high flexibility and electrical conductivity was obtained by decorating composites of reduced graphene oxide and carboxylate multi-walled carbon nanotubes onto a cellulose fabric substrate. The excellent enzyme embedding method using gel electrolyte showed a Michaelis-Menten constant of 1.46 mM, which implies that lactate dehydrogenase had a high enzymatic activity. The assembled self-powered lactate biosensor showed a sensitivity of 3.16 µW mM− 1 cm− 2 in the 0–10 mM linear range and a detection limit of 9.49 µM (S/N = 3). Meanwhile, the biosensor has good tensile flexural stability, selectivity, and long-term stability. The lactate self-powered biosensor recovered 99.66–108.43% of lactate from real sweat with a relative standard deviation of less than 4.65% when integrated into clothing. The biosensor is promising for sensing lactate in real sweat.

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Electrochemical and biosensing properties of an FAD-dependent glucose dehydrogenase from Trichoderma virens

Cited by 5 publications

References 42 publications

Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Recent Advances in Electrochemical Enzyme-Based Biosensors for Food and Beverage Analysis

Wearable cellulose textile matrix self-powered biosensor sensing lactate in human sweat

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