Electroacupuncture Improves Pregnancy Outcomes in Rats with Thin Endometrium by Promoting the Expression of Pinopode-Related Molecules

Xi, Jin; Cheng, Jie; Jin, Chun-chun; Liu, Jingyu; Shen, Zhen-ru; Xia, Liangjun; Li, Qian; Shen, Jie; Xia, Yun; Xu, Bin

doi:10.1155/2021/6658321

Cited by 19 publications

(13 citation statements)

References 59 publications

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“…Acupuncture, moxibustion, cupping, acupoint sticking, meridian acupoint massage, and other methods commonly used in traditional Chinese medicine have gradually achieved good auxiliary effects in the treatment of different diseases. In recent years, it has been reported that acupuncture can improve the receptivity of endometrium and promote the formation of pinopodes, leading to increased levels of integrin αvβ3, homeobox A10 (HOXA10), heparin-binding EGF-like growth factor (HBEGF), estrogen receptor alpha (ERα), and progesterone receptor (PR) [ 173 ]. Moreover, the effectiveness of electroacupuncture (EA) in assisted reproduction has also been confirmed [ [174] , [175] , [176] ].…”

Section: Discussionmentioning

confidence: 99%

Research progress of stem cell therapy for endometrial injury

Cen¹,

Zhang²,

Bai³

et al. 2022

Materials Today Bio

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Section: Discussionmentioning

confidence: 99%

Research progress of stem cell therapy for endometrial injury

Cen¹,

Zhang²,

Bai³

et al. 2022

Materials Today Bio

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“…In addition, higher pinopode expression was found in subjects with higher serum P. Compared with P, E did not seem to play a significant role in mediating the therapeutic effects of TEAS. Some animal experiments proved that acupuncture promotes the expression of endometrial LIF during the implantation period [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Transcutaneous electrical acupoint stimulation improves endometrial receptivity resulting in improved IVF-ET pregnancy outcomes in older women: a multicenter, randomized, controlled clinical trial

Zhu

Yang

et al. 2022

Reprod Biol Endocrinol

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Objective To examine the effects and mechanisms of transcutaneous electrical acupoint stimulation (TEAS) on pregnancy outcomes in women undergoing in vitro fertilization (IVF)-embryo transfer (ET). Design, setting, and participants This efficacy study was a multicenter, randomized, controlled clinical trial (RCT) in women receiving IVF-ET. The mechanistic study was a single-center RCT. Interventions The participants received TEAS vs. no TEAS treatment. Main outcome measures In the efficacy study, the primary outcomes were the rates of clinical pregnancy, embryo implantation, and live birth. In the mechanistic study, sex hormones and endometrial protein expression were examined. Results Ultimately, 739 participants were enrolled (367 and 372 in the TEAS and control groups, respectively). The clinical pregnancy rate was higher in the TEAS group than in the controls (55.1% vs. 46.7%, P = 0.03). There were no significant differences in embryo implantation, biochemical pregnancy, and live birth rates between the two groups (all P > 0.05) in the study population. In women > 35 years, the clinical pregnancy rates, embryo implantation rates and live birth rates in the TEAS and control groups were 48.9% vs. 23.7% (P = 0.004),30.8 vs. 13.9% (P = 0.001) and 34.0% vs. 19.7% (P = 0.06) respectively. In the mechanistic study with 120 participants, on the theoretical embryo implantation day, better developed endometrial pinopodes, elevated endometrial integrin α1β1/αVβ3, leukemia inhibitory factor, and elevated serum progesterone levels were found in the TEAS group compared with controls. Conclusion TEAS significantly improved the clinical pregnancy rate in women undergoing IVF-ET, especially in women of older age. It might be due to improved endometrial receptivity. Trial registration ChiCTR-TRC-13003950.

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“…Frequency is one of the key parameters of electroacupuncture stimulation. It has been reported that low frequency electroacupuncture improves endometrial receptivity in hyperovulation cycle [ 34 ], further, Wei Chen et al reported that low-frequency electroacupuncture facilitates implantation by activating the VEGFR2/PI3K/AKT and VEGFR2/ERK signaling pathways to enhance endometrial angiogenesis in a rat model of ovarian hyperstimulation [ 35 ], but that of high-frequency electroacupuncture to endometrial receptivity in hyperovulation cycle remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture improves endometrial receptivity through miRNA-223-3p-mediated regulation of leukemia inhibitory factor / signal transducer and activator of transcription 3 signaling pathway

You

Zhang

et al. 2022

Bioengineered

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Ovulation-inducing drugs such as endogenous steroids could reduce endometrial receptivity during the implantation window, resulting in lower clinical pregnancy rates and higher miscarriage rates. The present study employed electroacupuncture therapy along with different frequencies on elevating impaired endometrial receptivity to elucidate the mechanism therein. The rats were split up into seven groups of normal, model, low-frequency electroacupuncture (LF-EA), high-frequency electroacupuncture (HF-EA), LF-EA+anti-miRNA, HF-EA+anti-miRNA, and anti-miRNA. PCR assays were used to detect miR-223-3p expressions. The effects of electroacupuncture and miR-223-3p on rats’ endometrial membrane cell-drinking process in a manner of scanning electron microscopy were recorded. After that we observed on the electroacupuncture effects on the conditions of adhesion molecules and LIF/STAT3 signaling pathway with assays of immunofluorescence and Western Blot. This study was end up with dual luciferase assay, where combination of miR-233-3p onto the 3’-UTR sequence of LIF was determined. PCR assay demonstrated that HF-EA procured an inhibition in miR-223-3p expression, whereas scanning electron microscopy turned out that both electroacupuncture and miR-223-3p were capable of raising the amount of intrauterine pinocytosis and the number of blastocyst implantation in rats. Additionally, assays of Western Blot and immunofluorescence showed that therapy of electroacupuncture brought about decreasing expressions in adhesion molecules of E-cadherin, β-catenin and claudin-1 (CLDN1). We found that both electroacupuncture and miR-223-3p were able to fortify LIF/STAT3 signaling pathway, then the fact of miR-223-3p combination to LIF 3’-UTR sequence was validated via our dual-luciferase assay. Electroacupuncture therapy inhibited the miR-223-3p expression upon LIF/STAT3 signaling pathway to elevate endometrial receptivity.

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Electroacupuncture Improves Pregnancy Outcomes in Rats with Thin Endometrium by Promoting the Expression of Pinopode-Related Molecules

Cited by 19 publications

References 59 publications

Research progress of stem cell therapy for endometrial injury

Research progress of stem cell therapy for endometrial injury

Transcutaneous electrical acupoint stimulation improves endometrial receptivity resulting in improved IVF-ET pregnancy outcomes in older women: a multicenter, randomized, controlled clinical trial

Electroacupuncture improves endometrial receptivity through miRNA-223-3p-mediated regulation of leukemia inhibitory factor / signal transducer and activator of transcription 3 signaling pathway

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