Electroacupuncture at Fengchi (GB20) inhibits calcitonin gene-related peptide expression in the trigeminovascular system of a rat model of migraine

Zhao, Luopeng; Liu, Lu; Pei, Pei; Qu, Zhengyang; Zhu, Yupu; Wang, Linpeng

doi:10.4103/1673-5374.206652

Cited by 21 publications

(20 citation statements)

References 47 publications

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“…DES was administered to the Non-Acu, GB20+GB34, GB20, GB34 and Model groups using a stimulator (YC-2 stimulator; Chengdu Instrument Factory, Chengdu, Sichuan Province, China) in conscious rats. In accordance with methods described previously, DES, which consisted of 0.5 ms monophasic square wave pulses of 1.8–2.0 mA (intensity) and 20 Hz (frequency), was administered to rats for a 15 min period every other day (on Days 1, 3, 5, and 7), for a total of four sessions [31] (Figure 1(c)).…”

Section: Methodsmentioning

confidence: 99%

“…Acupuncture is known to be effective for alleviating pain and cutaneous allodynia and for preventing recurrent migraine [27, 28]. In our previous studies, we demonstrated that acupuncture could effectively alleviate mechanical hyperalgesia and normalizes the levels of abnormal biomarkers (c-Fos, CGRP, and serotonin [5-HT]) [29–31]. However, the peripheral trigeminovascular system has received little attention, especially with respect to vasoactive neurotransmitters and specific dural perivascular nerves.…”

Section: Introductionmentioning

confidence: 99%

“…However, the peripheral trigeminovascular system has received little attention, especially with respect to vasoactive neurotransmitters and specific dural perivascular nerves. In addition, most previously preclinical studies utilized a single acupuncture point (e.g., GB20 [Fengchi]) for the treatment of migraine [29–31]. However, multiple acupuncture points are often used in clinical practice (e.g., GB20 and Yanglingquan [GB34]) [28, 32–35].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Electroacupuncture on Hyperalgesia and Vasoactive Neurotransmitters in a Rat Model of Conscious Recurrent Migraine

Liu

Zhao

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Migraine onset is associated with the abnormal release of vasoactive neurotransmitters from perivascular nerves, and these neurotransmitters are involved in the pathophysiology of migraine. Hyperalgesia is a key feature of migraine, and accumulating evidence indicates that electroacupuncture (EA) at the single acupuncture point (Fengchi [GB20]) is effective in ameliorating hyperalgesia. In clinical practice, multiple acupuncture points are widely used, especially GB20 and Yanglingquan (GB34). However, the role played by vasoactive neurotransmitters in acupuncture antihyperalgesic effect at the single or multiple acupuncture points remains unknown. We aimed to determine whether EA would exert its antihyperalgesic effects by modulating vasoactive neurotransmitter release from the perivascular nerves. Furthermore, we examined whether targeting multiple acupuncture points would be more effective than targeting a single point in reducing hyperalgesia. The mechanical and thermal hyperalgesia were evaluated by measuring the facial and hind-paw mechanical withdrawal thresholds, tail-flick and hot-plate latencies. Plasma concentrations of vasoactive neurotransmitters were determined using rat-specific ELISA kits from jugular vein, including calcitonin gene-related peptide (CGRP), substance P (SP), vasoactive intestinal peptide (VIP), neuropeptide Y (NPY), pituitary adenylate cyclase-activating polypeptide (PACAP), nitric oxide (NO), and endothelin-1 (ET-1). The result suggested that EA significantly ameliorated the mechanical and thermal hyperalgesia, reduced c-Fos levels in the trigeminal ganglion, and attenuated plasma and dural levels of vasoactive neurotransmitters, especially in the multiple acupuncture points group (GB20+GB34). In conclusion, EA exerts antihyperalgesic effect in a rat model of conscious recurrent migraine, possibly via modulation of the vasoactive neurotransmitters. Furthermore, targeting multiple acupuncture points is more effective than targeting a single point in reducing hyperalgesia.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Electroacupuncture on Hyperalgesia and Vasoactive Neurotransmitters in a Rat Model of Conscious Recurrent Migraine

Liu

Zhao

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“… 16–19 Furthermore, Zhao et al confirmed in a migraine rat model that electro-acupuncture treatment can depress CGRP expression in the trigeminal ganglion, which plays a key role in inducing migraine attack. 20 Li et al also found that acupuncture restores the impaired descending pain modulatory system in migraine patients by decreasing the resting state functional connectivity between the periaqueductal grey and rostral anterior cingulate cortex/medial prefrontal cortex, which directly correlated with the intensity of pain during the migraine attack. 21 …”

Section: Introductionmentioning

confidence: 98%

Acupuncture for migraine: a protocol for a meta-analysis and meta-regression of randomised controlled trials

Gao

Giovanardi

et al. 2018

BMJ Open

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IntroductionAlthough the effectiveness of acupuncture for episodic migraine has been confirmed by multiple clinical trials and Cochrane systematic reviews, the mechanisms underlying the specific effect of acupuncture for migraine remain controversial. We aim to evaluate the effectiveness and safety of acupuncture for both episodic migraine and chronic migraine by meta-analysis and explore the possible factors influencing the specific effect of acupuncture for migraine by meta-regression.Methods and analysisWe will search for randomised control trials of acupuncture for migraine in the following eight databases: Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE, EMBASE, AMED (via OVID) and four Chinese databases (Chinese Biomedical Literature Database, China National Knowledge Infrastructure, Chinese Science and Technology Periodical Database and Wanfang Database) from inception to 31 December 2017. We will also search OpenSIGLE (opensigle.inist.fr) for conference abstracts. No language restriction will be applied. The selection of studies, data extraction and coding and assessment of risk of bias of the included studies will be conducted independently by two reviewers. Standard meta-analysis and, if appropriate, meta-regression will be performed using the R packages Meta and Metafor.Ethics and disseminationThe results of this meta-analysis and meta-regression will be disseminated through publication in a peer-reviewed journal and presented at a relevant conference. The data used in this meta-analysis will not contain individual patient data; therefore, ethical approval is not required.PROSPERO registration numberCRD42018087270.

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“…Considerable research has proven that the increase of neuronal discharges [15][16][17] and neurotransmitters such as glutamate, 18 neuropeptide Y, 19 C-fos, 20 and CGRP (Calcitonin gene-related peptide) 21 from the dorsal spinal cord neurons in TCC make a substantial contribution to attacks and mechanical CCH in migraine-affected rats. Otherwise, a dural inflammatory soup (IS) injection is used to initiate migraine attacks and induce hyperalgesia and allodynia in the receptive field of the periorbital region of trigeminal nerve, using mechanical pain threshold test with a Von-Frey filament.…”

Section: Introductionmentioning

confidence: 99%

<p>Prophylactic Electroacupuncture on the Upper Cervical Segments Decreases Neuronal Discharges of the Trigeminocervical Complex in Migraine-Affected Rats: An in vivo Extracellular Electrophysiological Experiment</p>

Liu

Zhao

et al. 2020

JPR

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Purpose: This rat experiment aims to demonstrate the efficacy of electrical acupuncture in preventing migraine attacks by stimulating the acupoint GB20. Introduction: Migraine, which takes 2ed at level four causes of GBD's disease hierarchy, becomes a public health issue. It is important for physicians to supplement their knowledge of its treatment and consider alternative methods of therapy, such as acupuncture. However, the neurobiological and pathophysiological mechanisms of this prophylactic effect were unclear. The trigeminocervical complex is thought to be an important relay station in migraine pathophysiology as the key nuclei of the trigeminovascular system and the brainstem descending pain modulation system. Methods: There were six groups involved in this study: control, model, electroacupuncture, non-acupoint electroacupuncture, saline+electroacupuncture and saline+non-acupoint electroacupuncture. We injected saline or inflammatory soup into dura mater to induce control or migraine in the rats. The mechanical pain threshold and the single-cell extraneural neurophysiology of the C1 spinal dorsal horn neurons in the trigeminocervical complex were detected. Results: Pre-electroacupuncture could significantly increase the mechanical pain threshold of the periorbital region receptive field of the trigeminal nerve and decrease the discharges of neurons in the trigeminocervical complex. Acupuncture also reversed the abnormal skin pain response of the periorbital region receptive field of the trigeminal nerve caused by lowintensity stimulation. Discussion: We believe that our study makes a significant contribution to the literature because it is the first of its kind to use GB20 to provide relief from migraine attacks and mechanical cephalic cutaneous hypersensitivity by regulating the neuronal discharge from trigeminocervical complex.

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Electroacupuncture at Fengchi (GB20) inhibits calcitonin gene-related peptide expression in the trigeminovascular system of a rat model of migraine

Cited by 21 publications

References 47 publications

Effect of Electroacupuncture on Hyperalgesia and Vasoactive Neurotransmitters in a Rat Model of Conscious Recurrent Migraine

Effect of Electroacupuncture on Hyperalgesia and Vasoactive Neurotransmitters in a Rat Model of Conscious Recurrent Migraine

Acupuncture for migraine: a protocol for a meta-analysis and meta-regression of randomised controlled trials

<p>Prophylactic Electroacupuncture on the Upper Cervical Segments Decreases Neuronal Discharges of the Trigeminocervical Complex in Migraine-Affected Rats: An in vivo Extracellular Electrophysiological Experiment</p>

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