Electrically tunable lens integrated with optical coherence tomography angiography for cerebral blood flow imaging in deep cortical layers in mice

Li, Yuandong; Tang, Ping; Song, Shaozhen; Rakymzhan, Adiya; Wang, Ke

doi:10.1364/ol.44.005037

Cited by 13 publications

(5 citation statements)

References 13 publications

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“…At the maximum imaging distance, image resolution severely decreases due to defocusing. Combining this system with a tunable lens might be a solution to maximize image quality throughout the entire oral cavity [ 49 ]. Moreover, while the proposed system can quickly perform the scans, it results in larger data sizes and longer computational times for reconstructing 3D OCT images.…”

Section: Discussionmentioning

confidence: 99%

High-speed, long-range and wide-field OCT for in vivo 3D imaging of the oral cavity achieved by a 600 kHz swept source laser

Shi,

Liu,

Wang

2024

Biomed. Opt. Express

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We report a high-speed, long-range, and wide-field swept-source optical coherence tomography (SS-OCT) system aimed for imaging microstructures and microcirculations in the oral cavity. This system operates at a scan speed of 600 kHz, delivering a wide imaging field of view at 42 × 42 mm2 and a ranging distance of 36 mm. To simultaneously meet the requirements of high speed and long range, it is necessary for the k-clock trigger signal to be generated at its maximum speed, which may induce non-linear phase response in electronic devices due to the excessive k-clock frequency bandwidth, leading to phase errors. To address this challenge, we introduced a concept of electrical dispersion and a global k-clock compensation approach to improve overall performance of the imaging system. Additionally, image distortion in the wide-field imaging mode is also corrected using a method based on distortion vector maps. With this system, we demonstrate comprehensive structural and blood flow imaging of the anterior oral cavity in healthy individuals. The high-speed, long-range, and wide-field SS-OCT system opens new opportunities for comprehensive oral cavity examinations and holds promise as a reliable tool for assessing oral health conditions.

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Section: Discussionmentioning

confidence: 99%

High-speed, long-range and wide-field OCT for in vivo 3D imaging of the oral cavity achieved by a 600 kHz swept source laser

Shi,

Liu,

Wang

2024

Biomed. Opt. Express

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“…However, the OCT probing beam spot was situated at the subsurface (at a depth of 200 µm from the skin surface) to cover the hypodermis to the top of muscle within the DOF, where the capillary sprouts in the wound's neovascularization were well-focused, compromising with the blurring of the deeper and superficial vessels outside the DOF. To overcome the DOF limitation, it is recommended to use an electrically tunable lens (ETL), providing fast and dynamic control of axial focusing of the probe beam, benefiting the expansion of the DOF by over 1000 µm [63].…”

Section: Discussionmentioning

confidence: 99%

Non-Invasive Monitoring of Cutaneous Wound Healing in Non-Diabetic and Diabetic Model of Adult Zebrafish Using OCT Angiography

Kim

Choi

2023

Bioengineering

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A diabetic wound presents a severe risk of infections and other complications because of its slow healing. Evaluating the pathophysiology during wound healing is imperative for wound care, necessitating a proper diabetic wound model and assay for monitoring. The adult zebrafish is a rapid and robust model for studying human cutaneous wound healing because of its fecundity and high similarities to human wound repair. OCTA as an assay can provide three-dimensional (3D) imaging of the tissue structure and vasculature in the epidermis, enabling monitoring of the pathophysiologic alterations in the zebrafish skin wound. We present a longitudinal study for assessing the cutaneous wound healing of the diabetic adult zebrafish model using OCTA, which is of importance for the diabetes research using the alternative animal models. We used non-diabetic (n = 9) and type 1 diabetes mellitus (DM) adult zebrafish models (n = 9). The full-thickness wound was generated on the fish skin, and the wound healing was monitored with OCTA for 15 days. The OCTA results demonstrated significant differences between diabetic and non-diabetic wound healing, involving delayed tissue remodeling and impaired angiogenesis for the diabetic wound, leading to slow wound recovery. The adult zebrafish model and OCTA technique may benefit long-term metabolic disease studies using zebrafish for drug development.

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“…79–81 Proof-of-principle measurements of blood flow have been taken from larger branches of principal cortical venules in the CC. 76…”

Section: Optical Coherence Tomographymentioning

confidence: 99%

“…[79][80][81] Proof-of-principle measurements of blood flow have been taken from larger branches of principal cortical venules in the CC. 76 OCTA has been used to study cerebrovascular diseases. For example, imaging of blood flow in pial and penetrating arterioles after middle cerebral artery occlusion have revealed how collateral arterioles redistribute blood flow in penetrating arterioles throughout the cortex.…”

Section: Optical Coherence Tomographymentioning

confidence: 99%

“…Prior studies showed that 1300 nm OCTA can be used to resolve microvascular structure in vivo from the pial surface to the CC in the mouse barrel cortex through a cranial window (Figure 2A). 76 A dynamic focusing approach was used by integrating an electrically tunable lens in the scanning optics to overcome the depth of focus limitation and enhance deep vascular imaging. The large branches of principal cortical venules are prominent at the interface between cortical layer 6 and underlying white matter and can be seen alongside smaller capillary-sized vessels (Figure 2A).…”

Section: Optical Coherence Tomographymentioning

confidence: 99%

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Deep Imaging to Dissect Microvascular Contributions to White Matter Degeneration in Rodent Models of Dementia

Stamenković¹,

Li²,

Waters

et al. 2023

Stroke

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The increasing socio-economic burden of Alzheimer disease (AD) and AD-related dementias has created a pressing need to define targets for therapeutic intervention. Deficits in cerebral blood flow and neurovascular function have emerged as early contributors to disease progression. However, the cause, progression, and consequence of small vessel disease in AD/AD-related dementias remains poorly understood, making therapeutic targets difficult to pinpoint. Animal models that recapitulate features of AD/AD-related dementias may provide mechanistic insight because microvascular pathology can be studied as it develops in vivo. Recent advances in in vivo optical and ultrasound-based imaging of the rodent brain facilitate this goal by providing access to deeper brain structures, including white matter and hippocampus, which are more vulnerable to injury during cerebrovascular disease. Here, we highlight these novel imaging approaches and discuss their potential for improving our understanding of vascular contributions to AD/AD-related dementias.

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Electrically tunable lens integrated with optical coherence tomography angiography for cerebral blood flow imaging in deep cortical layers in mice

Cited by 13 publications

References 13 publications

High-speed, long-range and wide-field OCT for in vivo 3D imaging of the oral cavity achieved by a 600 kHz swept source laser

High-speed, long-range and wide-field OCT for in vivo 3D imaging of the oral cavity achieved by a 600 kHz swept source laser

Non-Invasive Monitoring of Cutaneous Wound Healing in Non-Diabetic and Diabetic Model of Adult Zebrafish Using OCT Angiography

Deep Imaging to Dissect Microvascular Contributions to White Matter Degeneration in Rodent Models of Dementia

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