Electrical imaging of neuronal activity by multi-transistor-array (MTA) recording at 7.8 μm resolution

Lambacher, Armin; Jenkner, M.; Merz, M.; Eversmann, B.; Kaul, R. A.; Hofmann, Franz; Thewes, R.; Fromherz, Peter

doi:10.1007/s00339-004-2991-5

Cited by 94 publications

(79 citation statements)

References 22 publications

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“…For the default configuration, we measured every second column (128 ϫ 64 sensors) with a sampling frequency of 12 kHz for each sensor. In each experiment, sensor transistors were calibrated by applying an AC voltage (frequency, 70 Hz; amplitude, 3 mV peak-to-peak) to the bath electrode (Eversmann et al, 2003;Lambacher et al, 2004). The calibration voltage changes the electrical potential at the surface of the chip.…”

Section: Methodsmentioning

confidence: 99%

“…Here, we investigate these questions by recording the electrical activity in RGC populations of adult rd1 retinas (C3H and FVB/N) using a high-density multi-transistor array (MTA) (Eversmann et al, 2003;Lambacher et al, 2004). The array comprises 16,384 electrodes packed in 1 mm 2 , thus allowing for very high spatial electrical imaging of the interfaced in vitro retina.…”

Section: Introductionmentioning

confidence: 99%

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Network Oscillations in Rod-Degenerated Mouse Retinas

Menzler¹,

Zeck²

2011

J. Neurosci.

113

169

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In the mammalian retina, excitatory and inhibitory circuitries enable retinal ganglion cells (RGCs) to signal the occurrence of visual features to higher brain areas. This functionality disappears in certain diseases of retinal degeneration because of the progressive loss of photoreceptors. Recent work in a mouse model of retinal degeneration (rd1) found that, although some intraretinal circuitry is preserved and RGCs maintain characteristic physiological properties, they exhibit increased and aberrant rhythmic activity. Here, extracellular recordings were made to assess the degree of aberrant activity in adult rd1 retinas and to investigate the mechanism underlying such behavior. A multi-transistor array with thousands of densely packed sensors allowed for simultaneous recordings of spiking activity in populations of RGCs and of local field potentials (LFPs). The majority of identified RGCs displayed rhythmic (7-10 Hz) but asynchronous activity. The spiking activity correlated with the LFPs, whichreflectanaveragesynchronizedexcitatoryinputtotheRGCs.LFPsinitiatedfromrandompositionsandpropagatedacrosstheretina.They disappeared when ionotrophic glutamate receptors or electrical synapses were blocked. They persisted in the presence of other pharmacological blockers, including TTX and inhibitory receptor antagonists. Our results suggest that excitation-transmitted laterally through a network of electrically coupled interneurons-leads to large-scale retinal network oscillations, reflected in the rhythmic spiking of most rd1 RGCs. This result may explain forms of photopsias reported by blind patients, while the mechanism involved should be considered in future treatment strategies targeting the disease of retinitis pigmentosa.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Network Oscillations in Rod-Degenerated Mouse Retinas

Menzler¹,

Zeck²

2011

J. Neurosci.

113

169

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“…Significant noise reduction compared to the first generation of the MTA chips [15][16][17] is achieved by redesigning the terminating and isolation circuitry for digital control signals, the reference generation and potentiostat, the power supplies, and the external amplifiers. Furthermore, a novel timing protocol for the readout allows an enhanced sampling rate for the full frame of 16,384 sensor transistors.…”

Section: Chipmentioning

confidence: 99%

“…The problem of high resolution in two dimensions was solved with a multi-transistor array (MTA) that was fabricated by an extended complementary metal-oxidesemiconductor (CMOS) technology [15][16][17]. The array of 128 × 128 sensors had a pitch of 7.4 µm with the interconnect lines buried in the chip.…”

mentioning

confidence: 99%

“…Undefined free-floating MOSFET gate nodes and fixed pattern noise due to process mismatch were overcome by an autozeroing circuitry in each pixel. This MTA device successfully yielded dynamic electrical maps on 1 mm 2 of cultured snail neurons and of cultured brain slices [16,17]. A recording of cultured mammalian neurons was not possible with the first generation of MTA chips because the gate-referred RMS noise was too high due to the high bandwidth required for a complete sequential readout of the 16,384 sensors at a sampling frequency of 2 kHz for the whole array.…”

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confidence: 99%

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Identifying firing mammalian neurons in networks with high-resolution multi-transistor array (MTA)

et al. 2010

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The electrical activity of a network of mammalian neurons is mapped with a Multi-Transistor Array (MTA) fabricated with extended CMOS technology. The spatial resolution is 7.4 µm on an area of 1 mm 2 at a sampling frequency of 6 kHz for a complete readout of 16,384 sensor transistors. Action potentials give rise to extracellular voltages with amplitudes in a range of 500 µV. On the basis of the high resolution in space and time, correlation algorithms are used to identify single action potentials with amplitudes as low as about 200µV, and to assign the signals to the activity of individual neurons even in a dense network.

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The Neuron–Semiconductor Interface

Fromherz

2005

Bioelectronics

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Electrical imaging of neuronal activity by multi-transistor-array (MTA) recording at 7.8 μm resolution

Cited by 94 publications

References 22 publications

Network Oscillations in Rod-Degenerated Mouse Retinas

Network Oscillations in Rod-Degenerated Mouse Retinas

Identifying firing mammalian neurons in networks with high-resolution multi-transistor array (MTA)

The Neuron–Semiconductor Interface

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