Electric field effects on bacterial motility and chemotaxis

Eisenbach, Michael; Zimmerman, Jerald R.; Ciobotariu, Adina; Fischler, H.; Korenstein, Rafi

doi:10.1016/s0022-0728(83)80517-8

Cited by 6 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is noted that the formation of holes (pits) on the microbial cell membrane also was observed in several works performed in the presence of Ag + ions or Ag‐NPs, as confirmed by TEM studies reported in the literature . The fact that a very similar phenomenon (formation of pits and cell lysis) also was observed in the case where an external electrostatic force was applied to microbial cells, further reinforces the notion that the interaction between the microbial OM and surface of the Ag‐supported solid is of an electrostatic nature.…”

Section: Resultssupporting

confidence: 80%

New insights into the antimicrobial treatment of water on Ag‐supported solids

Theofilou

Constantinou

Chatziiona

et al. 2018

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND Silver (Ag) has been long known to be a strong antimicrobial agent and has been used as such either as AgNO3 or in the form of nanoparticles. The antimicrobial activity of nanosilver is believed to be due to free metal ion toxicity, the consequent generation of excess reactive oxygen species and inhibition of gene expression in several cells. RESULTS The antimicrobial activity of Ag/Al2O3 spheres was studied after suppression of free Ag ions by using a suitable complexing agent (Ag+ scavenger). It was found that Ag/Al2O3 retained its antimicrobial activity even after the addition of the Ag+ complexing agent, which is in contrast to the behaviour of an AgNO3 solution which became completely inactive. Initial/preliminary transmission electron microscopy and Fourier transform infrared studies indicate possible phospholipid residues on the Ag‐supported solid surface. •OH radicals were confirmed to be formed during the antimicrobial process. CONCLUSIONS The present work provides strong evidence that the antimicrobial property of Ag‐supported solids is not exclusively due to the dissolution of surface silver (free Ag+). A possible simplified mechanism is proposed in which the initiation of the antimicrobial reaction is proposed to be a heterogeneous intersurface process, which might include the interaction between the partially positively charged, surface silver atoms and the negatively charged outer membrane (OM) of microbes, and the subsequent activation of a free radical mechanism. Further study and confirmation of the above findings might be decisive for the development of novel Ag‐supported solids with limited metal surface dissolution but strong antimicrobial activity useful for the confrontation of particular environmental challenges. © 2018 Society of Chemical Industry

show abstract

Section: Resultssupporting

confidence: 80%

New insights into the antimicrobial treatment of water on Ag‐supported solids

Theofilou

Constantinou

Chatziiona

et al. 2018

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…(18)(19)(20), with cells showing defined trajectories and uniform movement toward an applied potential gradient, generated with a power supply, of 4 V/cm (18). Furthermore, application of electromagnetically induced electric fields was shown to promote motility while disrupting chemotaxis in Escherichia coli strains (21). In contrast, our study was designed to mimic the conditions that cells may encounter when using insoluble compounds for anaerobic respiration.…”

Section: Behavioral Responses Of S Oneidensis Mr-1 To Manganese and mentioning

confidence: 99%

Electrokinesis is a microbial behavior that requires extracellular electron transport

Harris

El‐Naggar²,

Bretschger³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

129

View full text Add to dashboard Cite

We report a previously undescribed bacterial behavior termed electrokinesis . This behavior was initially observed as a dramatic increase in cell swimming speed during reduction of solid MnO 2 particles by the dissimilatory metal-reducing bacterium Shewanella oneidensis MR-1. The same behavioral response was observed when cells were exposed to small positive applied potentials at the working electrode of a microelectrochemical cell and could be tuned by adjusting the potential on the working electrode. Electrokinesis was found to be different from both chemotaxis and galvanotaxis but was absent in mutants defective in electron transport to solid metal oxides. Using in situ video microscopy and cell tracking algorithms, we have quantified the response for different strains of Shewanella and shown that the response correlates with current-generating capacity in microbial fuel cells. The electrokinetic response was only exhibited by a subpopulation of cells closest to the MnO 2 particles or electrodes. In contrast, the addition of 1 mM 9,10-anthraquinone-2,6-disulfonic acid, a soluble electron shuttle, led to increases in motility in the entire population. Electrokinesis is defined as a behavioral response that requires functional extracellular electron transport and that is observed as an increase in cell swimming speeds and lengthened paths of motion that occur in the proximity of a redox active mineral surface or the working electrode of an electrochemical cell.

show abstract

“…Since chemotaxis and energy taxis in S. oneidensis and other organisms relies on CheA, the absence of a phenotype in the cheA‐3 mutant is surprising. Harris et al also eliminated galvanotaxis (taxis through electrical fields) as a possible cause of taxis to electrodes by showing that electrical fields induced by high voltage (≥1.2V) resulted in “slow uniform migration” dissimilar to swimming motility during taxis to electrodes (Eisenbach et al, 1983; Harris et al, 2010).…”

Section: Taxis In S Oneidensismentioning

confidence: 99%

Electrolocation? The evidence for redox‐mediated taxis in Shewanella oneidensis

Starwalt-Lee

El‐Naggar

Bond

et al. 2020

Molecular Microbiology

View full text Add to dashboard Cite

Shewanella oneidensis is a dissimilatory metal reducing bacterium and model for extracellular electron transfer (EET), a respiratory mechanism in which electrons are transferred out of the cell. In the last 10 years, migration to insoluble electron acceptors for EET has been shown to be nonrandom and tactic, seemingly in the absence of molecular or energy gradients that typically allow for taxis. As the ability to sense, locate, and respire electrodes has applications in bioelectrochemical technology, a better understanding of taxis in S. oneidensis is needed. While the EET conduits of S. oneidensis have been studied extensively, its taxis pathways and their interplay with EET are not yet understood, making investigation into taxis phenomena nontrivial. Since S. oneidensis is a member of an EET‐encoding clade, the genetic circuitry of taxis to insoluble acceptors may be conserved. We performed a bioinformatic analysis of Shewanella genomes to identify S. oneidensis chemotaxis orthologs conserved in the genus. In addition to the previously reported core chemotaxis gene cluster, we identify several other conserved proteins in the taxis signaling pathway. We present the current evidence for the two proposed models of EET taxis, “electrokinesis” and flavin‐mediated taxis, and highlight key areas in need of further investigation.

show abstract

Electric field effects on bacterial motility and chemotaxis

Cited by 6 publications

References 33 publications

New insights into the antimicrobial treatment of water on Ag‐supported solids

New insights into the antimicrobial treatment of water on Ag‐supported solids

Electrokinesis is a microbial behavior that requires extracellular electron transport

Electrolocation? The evidence for redox‐mediated taxis in Shewanella oneidensis

Contact Info

Product

Resources

About