Elecsys Insulin Assay: Free Insulin Determination and the Absence of Cross-Reactivity with Insulin Lispro

Insulin Instability in Parenteral Nutrition Admixtures

“…24,25 This method proved to be reliable and extremely effective in terms of specificity, linearity, sensitivity, accuracy, and precision. In fact, as previous comparative laboratory analyses have shown, [10][11][12][13] electrochemical immunoassays technology is much more reliable than previous chemiluminescent immunoassays, based on phosphate substrate, because it avoids every possible reaction interference. Moreover, sample manipulations, which are recognized contributors to insulin losses, can be avoided using this method.…”

“…Finally, linearity was evaluated by the correlation coefficient (ρ) of the immunoanalyzer calibration curve. [10][11][12][13] Statistics. Student t test using STATA 14 (Santa Monica, CA, USA) was used to compare 2-in-1 with 3-in-1 admixtures containing the same insulin amount, as well as each individual admixture at T0 and T24, where possible.…”

Interpreting insulin immunoassays during investigation of apparent spontaneous hypoglycaemia and insulin overdose

“…Insulin aspart has a single substitution of aspartic acid for proline at position B28, while the amino acids at positions 28 (Pro to Lys) and 29 (Lys to Pro) of the B chain are reversed in insulin lispro. 2,3 Insulin glulisine also has an amino acid substitution at the carboxy-terminal end of the B chain (Lys to Glu at B29) and also one at the amino-terminal end (Asp to Lys at B3). 4 These amino acid substitutions reduce hexamer formation, resulting in rapid absorption after subcutaneous injection, a faster onset and shorter duration of action.…”

“…Acute kidney injury (AKI) in hospitalised patients is strongly associated with adverse outcomes, including increased mortality. [1][2][3] It has been hypothesised that AKI in hospitalised patients is both a reflection of a critically unwell patient as well as an independent determinant of poor outcomes. 4 Early intervention for AKI may improve outcomes; however, referral to inpatient nephrology services prior to the initiation of dialysis is under-utilised.…”

The elevated gene expression level of the A2B adenosine receptor is associated with hyperglycemia in women with gestational diabetes mellitus

“…The biochemical assays were carried out with a COBAS INTEGRA analyzer (Roche, SA). Plasma insulin was quantified using Elecsys insulin assay (Roche Diagnostics GmbH, Mannheim, Germany) [32]. The homeostasis model assessment (HOMA) was used to calculate indices of insulin resistance (HOMA-IR) and β-cell function (HOMA-B) for each patient [33]:…”