2018
DOI: 10.1186/s13071-018-2738-3
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Abstract: BackgroundEhrlichia canis is a tick-borne bacterium that causes severe, life-threatening disease in dogs, being more prevalent in tropical and subtropical countries. Randomized studies conducted in Brazil indicate that the prevalence of E. canis infection in dogs ranges from 0.7% to over 50.0%. In a study conducted in northern Brazil, the prevalence was higher in dogs from urban areas, as compared to dogs from rural areas. In the present study, we investigated the exposure to Ehrlichia spp. infection in dogs f… Show more

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Cited by 18 publications
(8 citation statements)
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“…Ehrlichiosis, due to infection with E. canis , was firstly recognized in Brazil in the 1970s, and is prevalent in virtually all regions (for a comprehensive review on ehrlichiosis in Brazil, see Vieira et al [364]). The seroprevalence of infection varies between the southern, Central-West and northern-northeastern regions of Brazil, but it can be as high as 62.8% in asymptomatic and 78% in symptomatic dogs [38, 306, 309312, 324, 352, 364375]. Molecular prevalence for E. canis has been found in dogs at a range of 15–88% [316, 364].…”
Section: Country Filesmentioning
confidence: 99%
“…Ehrlichiosis, due to infection with E. canis , was firstly recognized in Brazil in the 1970s, and is prevalent in virtually all regions (for a comprehensive review on ehrlichiosis in Brazil, see Vieira et al [364]). The seroprevalence of infection varies between the southern, Central-West and northern-northeastern regions of Brazil, but it can be as high as 62.8% in asymptomatic and 78% in symptomatic dogs [38, 306, 309312, 324, 352, 364375]. Molecular prevalence for E. canis has been found in dogs at a range of 15–88% [316, 364].…”
Section: Country Filesmentioning
confidence: 99%
“…Leishmania spp. kDNA was detected by real-time PCR as described elsewhere [20,21], using the primers LEISH-1 (5′-AAC TTT TCT GGT CCT CCG GGT AG-3′) and LEISH-2 (5′-ACC CCC AGT TTC CCG CC-3′) and the TaqMan ® probe FAM-5′-AAA AAT GGG TGC AGA AAT-3′-non-fluorescent quencher-MGB [21]. Each reaction contained a final volume of 15 μl, including 7.5 μl of TaqMan ® Fast Advanced Master Mix (Applied Biosystems, Carlsbad, CA, USA), 1.35 μl of each primer (at a concentration of 900 nM), 0.3 μl of probe (at 200 nM), 2 μl of DNA sample, 2.5 μl of DNA-free water.…”
Section: Molecular Testingmentioning
confidence: 99%
“…As a result, there is very limited information about the annual incidence of VBP infections in dogs, in spite of the large number of cross-sectional studies available in the literature (e.g. [16][17][18][19][20]). Prevalence data cannot per se be used to infer incidence, also considering that seroconversion may take months to occur and that antibodies produced against certain pathogens may last for months.…”
Section: Introductionmentioning
confidence: 99%
“…We found a widespread occurrence of CVBP in rural, free-ranging dogs of central Chile, with three quarters of the dogs positive to at least one pathogen. Previous studies in other parts of the world showed that rural dogs are frequently exposed to or infected by different vector-borne pathogens [59,60]. The outdoor activity of these dogs exposes them to a range of vectors, which is, together with the absence of antiparasitic prophylactic management, the most likely reasons for our ndings.…”
Section: Discussionmentioning
confidence: 62%