Egibacter rhizosphaerae gen. nov., sp. nov., an obligately halophilic, facultatively alkaliphilic actinobacterium and proposal of Egibaceraceae fam. nov. and Egibacterales ord. nov.

Abstract

Egibacter rhizosphaerae gen. nov., sp. nov., an obligately halophilic, facultatively alkaliphilic actinobacterium and proposal of Egibaceraceae fam. nov. and Egibacterales ord. nov. T were Gram-stain-positive, non-motile and non-endospore-forming rods. Strain EGI 80759 T showed obligately halophilic growth with a tolerance to 8-25 % (w/v) NaCl (optimum growth at 10-12 %, w/v) and facultatively alkaliphilic growth within the pH range 7.0-11.0 (optimum growth at pH 9.0-10.0). Cell-wall hydrolysates of the isolate contained mesodiaminopimelic acid (peptidoglycan type A1c), with glucose, glucosamine, ribose and mannose as the major sugars. The major fatty acids identified were 10-methyl-C 17 : 0 , C 17 : 1 v8c and C 17 : 0 . The predominant menaquinone was MK-9(H 4 ). The G+C content of the genomic DNA was 72.1 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain EGI 80759 T clustered with members of the class Nitriliruptoria and showed highest 16S rRNA gene sequence similarities with Euzebya tangerina F10 T (90.3 %) and Nitriliruptor alkaliphilus ANL-iso2

Salsipaludibacter albus gen. nov., sp. nov., a novel actinobacterial strain isolate from a Portuguese solar saltern and proposal of Salsipaludibacteraceae fam. nov. and Salsipaludibacterales ord. nov.

“…S3). Altogether, these results suggest that AS10 T is halotolerant and alkalitolerant like its closely related strains [9, 10] (). Concerning vitamin requirements, AS10 T stopped growing after two transferences in agar medium without vitamins, showing the need for these supplements for growth.…”

“…S7). Accordingly, the predominant menaquinone in the closest related strains, Egicoccus halophilus [9], Egibacter rhizosphaerae [10], Euzebya rosea [60], Euzebya tangerina [8] and Euzebya pacifica [61] was also MK-9(H 4 ). Although no previous information about the respiratory quinones of N.…”

Euzebya pacifica sp. nov., a novel member of the class Nitriliruptoria

“…The class Nitriliruptoria belonging to the phylum Actinobacteria was established by Ludwig et al on the basis of the results of 16S rRNA gene sequence analysis and a distinct 16S rRNA gene phyletic lineage [1,2]. At the time of writing, the class Nitriliruptoria comprises four orders, including five species Egibacter rhizosphaerae, Egicoccus halophilus, Euzebya rosea, Euzebya tangerina and Nitriliruptor alkaliphilus [3][4][5][6][7]. Members of the class Nitriliruptoria inhabit diverse environments, including soda lake sediments, plant rhizosphere, saline-alkaline soil, seawater and marine fauna [3][4][5][6][7].…”

“…At the time of writing, the class Nitriliruptoria comprises four orders, including five species Egibacter rhizosphaerae, Egicoccus halophilus, Euzebya rosea, Euzebya tangerina and Nitriliruptor alkaliphilus [3][4][5][6][7]. Members of the class Nitriliruptoria inhabit diverse environments, including soda lake sediments, plant rhizosphere, saline-alkaline soil, seawater and marine fauna [3][4][5][6][7].…”

The Translation Initiation Factor 1A (TheIF1A) from Tamarix hispida Is Regulated by a Dof Transcription Factor and Increased Abiotic Stress Tolerance

“…Recently, a novel obligately halophilic, facultatively alkaliphilic actinobacterium, designated EGI 80759T, was isolated from the rhizosphere of T. hispida Willd, in Karamay, Xinjiang Province, northwestern China. Strain EGI 80759T exhibits obligately halophilic growth with tolerance to 8–25% (w/v) NaCl (optimum growth at 10–12%, w/v) and facultatively alkaliphilic growth within the pH range 7.0–11.0 (optimum growth at pH 9.0–10.0) (Zhang et al, 2016). Based on monitoring data of the groundwater level at typical sections in the lower reaches of Tarim River, the PSII photosynthetic activity of T. hispida under drought stress appears to decrease with increasing groundwater depth, and the increase in excess energy could result in a greater risk of photoinhibition.…”