Egg-Derived Peptide IRW Inhibits TNF-α-Induced Inflammatory Response and Oxidative Stress in Endothelial Cells

Abstract

Oxidative stress and vascular inflammatory response are key mediators of endothelial dysfunction that leads to cardiovascular diseases. A novel peptide, IRW, was previously characterized from egg protein with angiotensin converting enzyme inhibitory activity. The purpose of the study was to investigate the effects and molecular mechanisms of IRW on regulating inflammatory response in endothelial cells. The results showed that tumor necrosis factor-α (TNF-α) significantly increased the protein levels of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and monocyte chemotactic protein-1 (MCP-1), whereas pretreatment with IRW inhibited TNF-α-induced increases of ICAM-1, VCAM-1, and MCP-1 production in a concentration-dependent manner. IRW also reduced the levels of superoxide ions in the presence and absence of TNF-α. These results indicate the potential role of IRW in preventing cardiovascular disease as a functional food ingredient or nutraceutical.

Estradiol Modulates Tumor Necrosis Factor-Induced Endothelial Inflammation: Role of Tumor Necrosis Factor Receptor 2

“…We found that IĸBα was almost completely (90–91% decrease) degraded within 15 min of TNF treatment in both E 2 -treated and -untreated cells. Even after 60 min, IĸBα levels were only partially (38–54%) replenished, in agreement with previous work from our group [53]. In contrast, IĸBβ remained unaffected by TNF stimulation in the untreated cells, while it was partially degraded (42–48% decrease) on prolonged TNF treatment (45 and 60 min) only in the E 2 -treated cells.…”

Anti-diabetic activity peptides from albumin against α-glucosidase and α-amylase

“…Some prior work has shown that peptides derived from egg white protein exhibited biological activity, including angiotensin converting enzyme (ACE) inhibitory activity (Fujita, Sasaki, et al, 1995), antioxidant activity (Davalos, Miguel, et al, 2004), mineral-binding activity (Samaraweera, Zhang, et al, 2011), anti-inflammatory activity (Huang, Chakrabarti, et al, 2010), antimicrobial activity (Gong, Kalina, et al, 2009), anticoagulation properties (Yu et al, 2011a(Yu et al, , 2011b and anticancer properties (Yi, Kim, et al, 2003). Our previous work also suggested that the egg protein hydrolysates displayed anti-diabetic activity against glucosidase activity (Yu et al, 2011a(Yu et al, , 2011b.…”

Purification and characterization of antioxidant peptides from cooked eggs using a dynamic in vitro gastrointestinal model in vascular smooth muscle A7r5 cells

“…1, the whole egg digests significantly reduced the superoxide level in VSMCs, confirming egg as a potential antioxidant commodity. 20,27,28 Among the three fractions, F1 showed a significant higher antioxidant activity (p < 0.05) at 2.5 mg/mL compared to the untreated (Fig. 1).…”